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Literature summary extracted from

  • Bovetto, L.J.; Backer, D.P.; Villette, J.R.; Sicard, P.J.; Bouquelet, S.J.L.
    Cyclomaltodextrin glucanotransferase from Bacillus circulans E 192. I. Purification and characterization of the enzyme (1992), Biotechnol. Appl. Biochem., 15, 48-58.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.19 synthesis
-
 Geobacillus stearothermophilus
2.4.1.19 synthesis
-
 Paenibacillus macerans
2.4.1.19 synthesis
-
 Alkalihalobacillus alcalophilus
2.4.1.19 synthesis industrial strain E 192, industrial production of cyclodextrins Niallia circulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.19 0.043
-
 soluble starch
-
 Niallia circulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.19 extracellular
-
 Paenibacillus macerans
-
-
2.4.1.19 extracellular strain E 192 Niallia circulans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.19 Ca2+ protects enzyme from activity loss Geobacillus stearothermophilus
2.4.1.19 Ca2+ protects enzyme from activity loss Niallia circulans
2.4.1.19 Ca2+ protects enzyme from activity loss Paenibacillus macerans
2.4.1.19 Ca2+ protects enzyme from activity loss Alkalihalobacillus alcalophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.19 33500
-
 E 192, gel filtration superose 12 column Niallia circulans
2.4.1.19 48500
-
 E 192, Fractogel TSK HW 55 S Niallia circulans
2.4.1.19 78000
-
 E 192, 2 isozymes, gel filtration, SDS-PAGE Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Alkalihalobacillus alcalophilus
-
-
-
2.4.1.19 Bacillus ohbensis
-
-
-
2.4.1.19 Geobacillus stearothermophilus
-
-
-
2.4.1.19 Klebsiella pneumoniae
-
-
-
2.4.1.19 Micrococcus sp.
-
-
-
2.4.1.19 Niallia circulans
-
 B. sp. var. alkalophilus No. 38-2, ATCC 21783
-
2.4.1.19 Niallia circulans
-
 no. 8
-
2.4.1.19 Niallia circulans
-
 E 192
-
2.4.1.19 Niallia circulans
-
 ATCC 9995
-
2.4.1.19 Niallia circulans 8
-
 no. 8
-
2.4.1.19 Niallia circulans E 192
-
 E 192
-
2.4.1.19 Paenibacillus macerans
-
-
-
2.4.1.19 Priestia megaterium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 E 192 Niallia circulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.19 88
-
 E 192, dextrinizing-like activity Niallia circulans
2.4.1.19 183.8
-
 E 192, cyclodextrin synthesis Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 alpha-cyclodextrin + glycosyl acceptor
-
 Geobacillus stearothermophilus beta-cyclodextrin + maltooligosaccharide
-
 r
2.4.1.19 alpha-cyclodextrin + glycosyl acceptor
-
 Niallia circulans beta-cyclodextrin + maltooligosaccharide
-
 r
2.4.1.19 alpha-cyclodextrin + glycosyl acceptor
-
 Paenibacillus macerans beta-cyclodextrin + maltooligosaccharide
-
 r
2.4.1.19 alpha-cyclodextrin + glycosyl acceptor
-
 Niallia circulans E 192 beta-cyclodextrin + maltooligosaccharide
-
 r
2.4.1.19 alpha-cyclodextrin + glycosyl acceptor
-
 Niallia circulans 8 beta-cyclodextrin + maltooligosaccharide
-
 r
2.4.1.19 Glucidex 12 + glycosyl acceptor E 192 Niallia circulans beta-cyclodextrin
-
 r
2.4.1.19 Glucidex 2B + glycosyl acceptor E 192 Niallia circulans beta-cyclodextrin
-
 r
2.4.1.19 Glucidex 2B + glycosyl acceptor E 192 Niallia circulans E 192 beta-cyclodextrin
-
 r
2.4.1.19 Glucidex 2B + glycosyl acceptor E 192 Niallia circulans 8 beta-cyclodextrin
-
 r
2.4.1.19 glycogen + acceptor
-
 Geobacillus stearothermophilus beta-cyclodextrin
-
 ?
2.4.1.19 glycogen + acceptor
-
 Paenibacillus macerans beta-cyclodextrin
-
 r
2.4.1.19 glycogen + acceptor E 192 Niallia circulans beta-cyclodextrin
-
 r
2.4.1.19 maltoheptaose + glycosyl acceptor E 192 Niallia circulans beta-cyclodextrin
-
 r
2.4.1.19 maltohexaose + glycosyl acceptor E 192 Niallia circulans beta-cyclodextrin
-
 r
2.4.1.19 maltose + glycosyl acceptor
-
 Geobacillus stearothermophilus beta-cyclodextrin
-
 r
2.4.1.19 maltose + glycosyl acceptor E 192, poor substrate Niallia circulans beta-cyclodextrin
-
 r
2.4.1.19 maltotriose + glycosyl acceptor
-
 Geobacillus stearothermophilus beta-cyclodextrin
-
 r
2.4.1.19 maltotriose + glycosyl acceptor E 192 Niallia circulans beta-cyclodextrin
-
 r
2.4.1.19 additional information
-
 Geobacillus stearothermophilus ?
-
 ?
2.4.1.19 additional information
-
 Paenibacillus macerans ?
-
 ?
2.4.1.19 additional information
-
 Alkalihalobacillus alcalophilus ?
-
 ?
2.4.1.19 additional information glucose is no substrate Niallia circulans ?
-
 ?
2.4.1.19 additional information glucose is no substrate Niallia circulans E 192 ?
-
 ?
2.4.1.19 additional information glucose is no substrate Niallia circulans 8 ?
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Geobacillus stearothermophilus cyclodextrins
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Niallia circulans cyclodextrins
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus macerans cyclodextrins
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Micrococcus sp. cyclodextrins
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Niallia circulans E 192 cyclodextrins
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Niallia circulans 8 cyclodextrins
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Priestia megaterium beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus macerans beta-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus ohbensis beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor E 192, waxy maize starch is the best substrate, wheat starch, corn starch, potato starch Niallia circulans beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor E 192, waxy maize starch is the best substrate, wheat starch, corn starch, potato starch Niallia circulans E 192 beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor E 192, waxy maize starch is the best substrate, wheat starch, corn starch, potato starch Niallia circulans 8 beta-cyclodextrin
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.19 monomer
-
 Paenibacillus macerans
2.4.1.19 monomer 1 * 78000, E 192, SDS-PAGE Niallia circulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 60
-
-
 Geobacillus stearothermophilus
2.4.1.19 60
-
-
 Paenibacillus macerans
2.4.1.19 60
-
-
 Bacillus ohbensis
2.4.1.19 60
-
 E 192 Niallia circulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.19 40 60 strain E 192, heat labile, rapid inactivation at temperatures above 45, remaining activity is 75% after 20 min at 40°C, 10% at 50°C and only 5% after 1 min at 60°C, protected by substrate and Ca2+ enzyme is stable for at least 24 h at pH 6.0, very stable at pH 7.0, 48 h without any loss of activity Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 5.5
-
-
 Geobacillus stearothermophilus
2.4.1.19 5.5
-
-
 Niallia circulans
2.4.1.19 5.5
-
-
 Paenibacillus macerans
2.4.1.19 5.5
-
-
 Bacillus ohbensis
2.4.1.19 5.5 5.8 strain E 192 Niallia circulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.19 6 9 strain E 192, only 14% decrease in activity Niallia circulans
2.4.1.19 7 8 strain E 192, maximum stability, only 14% decrease in activity at pH 7.0 Niallia circulans