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Literature summary extracted from

  • Greenberg, E.; Preiss, J.
    Biosynthesis of bacterial glycogen. II. Purification and properties of the adenosine diphosphoglucose:glycogen transglucosylase of Arthrobacter specise NRRL B1973 (1965), J. Biol. Chem., 240, 2341-2348.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.21 Bovine plasma albumin activation Arthrobacter sp.
2.4.1.21 glutathione activation Arthrobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.21 ADP
-
 Arthrobacter sp.
2.4.1.21 p-chloromercuribenzoate 0.002 mM, 50% inhibition, 0.01 mM, 90% inhibition Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.21 additional information
-
 additional information
-
 Arthrobacter sp.
2.4.1.21 0.000164
-
 ADPglucose
-
 Arthrobacter sp.
2.4.1.21 0.000272
-
 dADPglucose
-
 Arthrobacter sp.
2.4.1.21 0.000864
-
 glycogen Arthrobacter glycogen Arthrobacter sp.
2.4.1.21 0.015
-
 maltotriose
-
 Arthrobacter sp.
2.4.1.21 0.077
-
 maltose
-
 Arthrobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.21 soluble
-
 Arthrobacter sp.
-
-
2.4.1.21 starch grain
-
 Arthrobacter sp. 43036
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.21 K+ 20-30% activation Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.21 Arthrobacter sp.
-
 NRRL B1973
-
2.4.1.21 Arthrobacter sp. NRRL B1973
-
 NRRL B1973
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.21 ammonium sulfate, calcium phosphate gel, DEAE-cellulose Arthrobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.21 0.93
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan citrate independent enzyme requires a glucan primer e.g. rabbit liver glycogen Arthrobacter sp. ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan glucosyl acceptors: Arthrobacter glycogen, potato starch, maltose and maltotriose Arthrobacter sp. ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan glucosyl acceptor: amylose Arthrobacter sp. ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan glucosyl acceptor: amylopectin Arthrobacter sp. ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan citrate independent enzyme requires a glucan primer e.g. rabbit liver glycogen Arthrobacter sp. NRRL B1973 ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan glucosyl acceptors: Arthrobacter glycogen, potato starch, maltose and maltotriose Arthrobacter sp. NRRL B1973 ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan glucosyl acceptor: amylose Arthrobacter sp. NRRL B1973 ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan glucosyl acceptor: amylopectin Arthrobacter sp. NRRL B1973 ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + glycogen
-
 Arthrobacter sp. ADP + ?
-
 ?
2.4.1.21 ADP-glucose + maltose
-
 Arthrobacter sp. ADP + maltotriose + maltotetraose
-
 ?
2.4.1.21 ADP-glucose + maltotriose
-
 Arthrobacter sp. ADP + maltotetraose
-
 ?
2.4.1.21 dADPglucose + alpha-1,4-polyglucan
-
 Arthrobacter sp. dADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 dADPglucose + alpha-1,4-polyglucan
-
 Arthrobacter sp. NRRL B1973 dADP + alpha-1,4-polyglucan
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.21 6 9
-
 Arthrobacter sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.21 0.08
-
 ADP
-
 Arthrobacter sp.