EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.25 | 2-mercaptoethanol | D-enzyme, slight inhibition | Pseudomonas stutzeri | |
2.4.1.25 | cyclomaltohexaose | inhibition 25% | Pseudomonas stutzeri | |
2.4.1.25 | dithiothreitol | D-enzyme, slight inhibition | Pseudomonas stutzeri | |
2.4.1.25 | EDTA | D-enzyme, slight inhibition | Pseudomonas stutzeri | |
2.4.1.25 | Fe2+ | D-enzyme, 50% inhibition | Pseudomonas stutzeri | |
2.4.1.25 | Hg2+ | D-enzyme | Pseudomonas stutzeri | |
2.4.1.25 | methyl-alpha-D-glucoside | competitive inhibition | Escherichia coli | |
2.4.1.25 | p-chloromercuribenzoic acid | D-enzyme, 30% inhibition | Pseudomonas stutzeri | |
2.4.1.25 | phenyl-beta-D-glucoside | competitive inhibition | Escherichia coli | |
2.4.1.25 | Tris | D-enzyme, 50 mM, 35% inhibition | Pseudomonas stutzeri | |
2.4.1.25 | Zn2+ | D-enzyme | Pseudomonas stutzeri |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.25 | 7.1 | - |
maltotetraose | - |
Pseudomonas stutzeri | |
2.4.1.25 | 8.3 | - |
maltose | D-enzyme | Pseudomonas stutzeri |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.25 | 38000 | - |
1 * 38000 + 1 * 47000, amylomaltase | Pseudomonas stutzeri |
2.4.1.25 | 47000 | - |
1 * 38000 + 1 * 47000, amylomaltase | Pseudomonas stutzeri |
2.4.1.25 | 52000 | - |
1 * 52000 + 1 * 63000, D-enzyme | Pseudomonas stutzeri |
2.4.1.25 | 63000 | - |
1 * 52000 + 1 * 63000, D-enzyme | Pseudomonas stutzeri |
2.4.1.25 | 71000 | - |
- |
Escherichia coli |
2.4.1.25 | 74000 | - |
amylomaltase, gel filtration | Pseudomonas stutzeri |
2.4.1.25 | 115000 | - |
D-enzyme, gel filtration | Pseudomonas stutzeri |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.25 | Bacillus subtilis | - |
- |
- |
2.4.1.25 | Escherichia coli | - |
- |
- |
2.4.1.25 | Escherichia coli ML 30 | - |
- |
- |
2.4.1.25 | Pseudomonas stutzeri | - |
NRRL B3389 | - |
2.4.1.25 | Pseudomonas stutzeri NRRL B3389 | - |
NRRL B3389 | - |
2.4.1.25 | Solanum tuberosum | - |
potato | - |
2.4.1.25 | Streptococcus sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.25 | both amylomaltase and D-enzyme | Pseudomonas stutzeri |
2.4.1.25 | E. coli ML 30, partially | Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.1.25 | 4°C, stored as a suspension in 3.2 M ammonium sulfate, no significant loss of activity for at least 1 month | Pseudomonas stutzeri |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.25 | maltoheptaose + maltotriose | D-enzyme | Pseudomonas stutzeri | maltononaose + D-glucose | - |
? | |
2.4.1.25 | maltohexaose + maltodextrin | only amylomaltase, no polymers larger than the initial maltodextrin substrate, maltohexaose is a good donor substrate, but unable to function as an acceptor | Pseudomonas stutzeri | D-glucose + maltopentaose | - |
? | |
2.4.1.25 | maltononaose + maltotriose | D-enzyme | Pseudomonas stutzeri | maltoundecaose + D-glucose | - |
? | |
2.4.1.25 | maltopentaose + maltodextrin | - |
Pseudomonas stutzeri | maltose + higher dextrins | - |
? | |
2.4.1.25 | maltopentaose + maltotriose | D-enzyme | Pseudomonas stutzeri | maltoheptaose + D-glucose | - |
? | |
2.4.1.25 | maltopentaose + maltotriose | amylomaltose | Pseudomonas stutzeri | homologous 1,4-alpha-D-glucans | - |
? | |
2.4.1.25 | maltose + maltose | - |
Escherichia coli | maltotriose + glucose | - |
? | |
2.4.1.25 | maltose + maltose | D-enzyme | Pseudomonas stutzeri | maltotriose + glucose | - |
? | |
2.4.1.25 | maltose + maltose | D-enzyme | Pseudomonas stutzeri NRRL B3389 | maltotriose + glucose | - |
? | |
2.4.1.25 | maltose + maltose | - |
Escherichia coli ML 30 | maltotriose + glucose | - |
? | |
2.4.1.25 | maltotetraose + maltodextrin | - |
Pseudomonas stutzeri | maltose + higher dextrins | - |
? | |
2.4.1.25 | maltotetraose + maltotriose | D-enzyme | Pseudomonas stutzeri | glucose + maltoheptaose + maltodecaose | - |
? | |
2.4.1.25 | maltotetraose + maltotriose | amylomaltose | Pseudomonas stutzeri | homologous 1,4-alpha-glucans | - |
? | |
2.4.1.25 | maltotetraose + maltotriose | - |
Solanum tuberosum | D-glucose + maltooligosaccharides | - |
? | |
2.4.1.25 | maltotetraose + maltotriose | amylomaltose, most active with maltotetraose | Pseudomonas stutzeri | D-glucose + maltooligosaccharides | - |
? | |
2.4.1.25 | maltotriose + maltodextrin | - |
Pseudomonas stutzeri | maltose + higher dextrins | - |
? | |
2.4.1.25 | maltotriose + maltodextrin | - |
Pseudomonas stutzeri NRRL B3389 | maltose + higher dextrins | - |
? | |
2.4.1.25 | maltotriose + maltodextrin | amylomaltase | Pseudomonas stutzeri | maltopentaose + ? | - |
? | |
2.4.1.25 | maltotriose + maltodextrin | amylomaltase | Pseudomonas stutzeri NRRL B3389 | maltopentaose + ? | - |
? | |
2.4.1.25 | maltotriose + maltotriose | D-enzyme | Pseudomonas stutzeri | maltopentaose + D-glucose | - |
? | |
2.4.1.25 | maltotriose + maltotriose | D-enzyme | Pseudomonas stutzeri | D-glucose + maltopentaose + maltoheptaose + maltononaose + maltoundecaose | - |
? | |
2.4.1.25 | maltotriose + maltotriose | amylomaltose | Pseudomonas stutzeri | homologous alpha-1,4-D-glucans | - |
? | |
2.4.1.25 | additional information | - |
Escherichia coli | ? | - |
? | |
2.4.1.25 | additional information | maltose is not a donor substrate, only weak acceptor activity | Solanum tuberosum | ? | - |
? | |
2.4.1.25 | additional information | D-enzyme, maltohexaose is no initial substrate | Pseudomonas stutzeri | ? | - |
? | |
2.4.1.25 | additional information | 2 glycosyltransferases, amylomaltase and D-enzyme | Pseudomonas stutzeri | ? | - |
? | |
2.4.1.25 | additional information | D-enzyme, maltohexaose is no initial substrate | Pseudomonas stutzeri NRRL B3389 | ? | - |
? | |
2.4.1.25 | additional information | 2 glycosyltransferases, amylomaltase and D-enzyme | Pseudomonas stutzeri NRRL B3389 | ? | - |
? | |
2.4.1.25 | additional information | - |
Escherichia coli ML 30 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.25 | dimer | 1 * 38000 + 1 * 47000, amylomaltase | Pseudomonas stutzeri |
2.4.1.25 | dimer | 1 * 52000 + 1 * 63000, D-enzyme | Pseudomonas stutzeri |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.25 | 30 | - |
D-enzyme | Pseudomonas stutzeri |
2.4.1.25 | 37 | - |
amylomaltase | Pseudomonas stutzeri |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.25 | 37 | 42 | D-enzyme, activity decreases rapidly above 37°C and is almost completely lost at 42°C | Pseudomonas stutzeri |
2.4.1.25 | 50 | - |
amylomaltase, rapidly inactivated at temperatures above | Pseudomonas stutzeri |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.25 | 6.9 | - |
- |
Escherichia coli |
2.4.1.25 | 7.6 | 7.7 | both amylomaltase and D-enzyme | Pseudomonas stutzeri |