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Literature summary extracted from

  • Lin, T.P.; Preiss, J.
    Characterization of D-enzyme (4-alpha-glucanotransferase) in Arabidopsis leaf (1988), Plant Physiol., 86, 260-265.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.25 aplanin Bay e 4609, pseudooligosaccharide resembling amylose with a hydroxymethylconduritol unit and a 4-amino-4-deoxy-D-chinorose residue linked to a varying number of alpha-D-glucose units from 7-30 Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Arabidopsis thaliana
-
Columbia wild-type
-
2.4.1.25 Daucus carota
-
carrot
-
2.4.1.25 Solanum lycopersicum
-
tomato
-
2.4.1.25 Spinacia oleracea
-
spinach
-
2.4.1.25 Vicia faba
-
broad bean, L.major
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25
-
Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.25 leaf
-
Spinacia oleracea
-
2.4.1.25 leaf
-
Arabidopsis thaliana
-
2.4.1.25 root
-
Daucus carota
-
2.4.1.25 tuber
-
Vicia faba
-
2.4.1.25 tuber
-
Arabidopsis thaliana
-
2.4.1.25 tuber
-
Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.25 1.13
-
D2 Arabidopsis thaliana
2.4.1.25 4.87
-
D1 Arabidopsis thaliana

Storage Stability

EC Number Storage Stability Organism
2.4.1.25 4°C, stable in 20 mM bis-Tris-propane buffer, pH 6.5, containing 10% glycerol, no loss of activity over 6 months Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 maltodextrin + D-glucose
-
Arabidopsis thaliana maltooligosaccharides
-
?
2.4.1.25 maltoheptaose + maltopentaose
-
Arabidopsis thaliana maltooligosaccharides
-
?
2.4.1.25 maltotetraose + maltopentaose
-
Arabidopsis thaliana maltooligosaccharides
-
?
2.4.1.25 maltotriose + maltotriose
-
Arabidopsis thaliana maltopentaose + D-glucose
-
?
2.4.1.25 additional information 2 major forms, D1 and D2 Arabidopsis thaliana ?
-
?
2.4.1.25 additional information glucose is the major product of the D-enzyme reaction Vicia faba ?
-
?
2.4.1.25 additional information glucose is the major product of the D-enzyme reaction Spinacia oleracea ?
-
?
2.4.1.25 additional information glucose is the major product of the D-enzyme reaction Arabidopsis thaliana ?
-
?
2.4.1.25 additional information glucose is the major product of the D-enzyme reaction Daucus carota ?
-
?
2.4.1.25 additional information glucose is the major product of the D-enzyme reaction Solanum lycopersicum ?
-
?
2.4.1.25 additional information amylopectin is no substrate, maltose is not a product Arabidopsis thaliana ?
-
?
2.4.1.25 starch + glucose
-
Spinacia oleracea maltotriose + ?
-
?
2.4.1.25 starch + glucose
-
Arabidopsis thaliana maltotriose + ?
-
?
2.4.1.25 starch + glucose
-
Daucus carota maltotriose + ?
-
?
2.4.1.25 starch + glucose
-
Solanum lycopersicum maltotriose + ?
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.25 60
-
loses activity when incubated for 10 min Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 5.5
-
-
Daucus carota
2.4.1.25 5.5
-
-
Solanum lycopersicum
2.4.1.25 6.5
-
-
Arabidopsis thaliana

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.25 4
-
no activity detected Arabidopsis thaliana