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Literature summary extracted from

  • Terada, Y.; Fujii, K.; Takaha, T.; Okada, S.
    Thermus aquaticus ATCC 33923 amylomaltase gene cloning and expression and enzyme characterization: production of cycloamylose (1999), Appl. Environ. Microbiol., 65, 910-915.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.25 nutrition cycloamylose will be used in the food, pharmaceutical and chemical industries Thermus aquaticus
2.4.1.25 synthesis cycloamylose will be used in the food, pharmaceutical and chemical industries Thermus aquaticus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25
-
 Escherichia coli
2.4.1.25
-
 Solanum tuberosum
2.4.1.25
-
 Streptococcus pneumoniae
2.4.1.25
-
 Clostridium butyricum
2.4.1.25
-
 Chlamydia psittaci
2.4.1.25
-
 Thermus aquaticus
2.4.1.25 cloned and sequenced and overexpressed in Escherichia coli Thermus aquaticus
2.4.1.25 putative gene identified in the genome Mycobacterium tuberculosis
2.4.1.25 putative gene identified in the genome Borreliella burgdorferi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.25 57220
-
 amino acid sequence Thermus aquaticus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Aquifex aeolicus
-
-
-
2.4.1.25 Borreliella burgdorferi
-
-
-
2.4.1.25 Chlamydia psittaci
-
-
-
2.4.1.25 Clostridium butyricum
-
 NCIMB 7423
-
2.4.1.25 Clostridium butyricum NCIMB 7423
-
 NCIMB 7423
-
2.4.1.25 Escherichia coli
-
-
-
2.4.1.25 Haemophilus influenzae
-
-
-
2.4.1.25 Hordeum vulgare
-
 barley
-
2.4.1.25 Mycobacterium tuberculosis
-
-
-
2.4.1.25 Solanum tuberosum
-
 potato
-
2.4.1.25 Streptococcus pneumoniae
-
-
-
2.4.1.25 Synechocystis sp.
-
-
-
2.4.1.25 Thermococcus litoralis
-
-
-
2.4.1.25 Thermus aquaticus
-
 ATCC 33923
-
2.4.1.25 Thermus aquaticus B7A9X4 ATCC 33923, nucleotide sequence accession number, deposited in DDBJ/EMBL/GenBank
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25
-
 Hordeum vulgare
2.4.1.25
-
 Thermococcus litoralis
2.4.1.25 native and recombinant enzyme Thermus aquaticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.25 tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.25 2.9
-
-
 Thermus aquaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 1,4-alpha-D-glucan + 1,4-alpha-D-glucan
-
 Thermus aquaticus maltooligosaccharides
-
 ?
2.4.1.25 1,4-alpha-D-glucan + 1,4-alpha-D-glucan
-
 Escherichia coli maltooligosaccharides
-
 ?
2.4.1.25 1,4-alpha-D-glucan + 1,4-alpha-D-glucan
-
 Solanum tuberosum maltooligosaccharides
-
 ?
2.4.1.25 1,4-alpha-D-glucan + glucose
-
 Thermus aquaticus maltooligosaccharides
-
 ?
2.4.1.25 1,4-alpha-D-glucan + glucose
-
 Escherichia coli maltooligosaccharides
-
 ?
2.4.1.25 1,4-alpha-D-glucan + glucose
-
 Solanum tuberosum maltooligosaccharides
-
 ?
2.4.1.25 amylose + maltopentaose
-
 Thermus aquaticus cyclic alpha-1,4-glucan cycloamylose ?
2.4.1.25 amylose + maltopentaose
-
 Solanum tuberosum cyclic alpha-1,4-glucan cycloamylose ?
2.4.1.25 amylose + maltopentaose
-
 Thermococcus litoralis cyclic alpha-1,4-glucan cycloamylose ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.25 75
-
-
 Thermus aquaticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.25 85
-
 stable up to, retains 50% activity after incubation at 80°C for 24 h, loses all activity after incubation at 100°C for 10 min Thermus aquaticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 5.5 6
-
 Thermus aquaticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.25 4 10
-
 Thermus aquaticus