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Literature summary extracted from

  • Marechal, L.R.; Goldemberg, S.H.
    Uridine diphosphate glucose-beta-1,3-glucan-beta-3-glucosyltransferase from Euglena gracilis (1964), J. Biol. Chem., 239, 3163-3167.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.34 cellobiose stimulate Euglena gracilis
2.4.1.34 cellobiosylglucose stimulate Euglena gracilis
2.4.1.34 D-glucosides stimulate Euglena gracilis
2.4.1.34 laminaribiose stimulate Euglena gracilis
2.4.1.34 additional information enzyme shows activity without addition of a primer Euglena gracilis
2.4.1.34 Salicin stimulate Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.34 Glycylglycine buffer 0.75 M Euglena gracilis
2.4.1.34 KCl
-
 Euglena gracilis
2.4.1.34 Na2SO4
-
 Euglena gracilis
2.4.1.34 NaCl
-
 Euglena gracilis
2.4.1.34 Natural inhibitor in green Euglena cells
-
 Euglena gracilis
2.4.1.34 p-hydroxymercuribenzoate
-
 Euglena gracilis
2.4.1.34 phosphoenolpyruvate
-
 Euglena gracilis
2.4.1.34 UDP
-
 Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.34 0.6
-
 UDPglucose
-
 Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n Euglena gracilis
-
 UDP + (1,3-beta-D-glucosyl)n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.34 Euglena gracilis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
2.4.1.34 -14°C, 2 weeks, 20% loss of activity Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n
-
 Euglena gracilis UDP + (1,3-beta-D-glucosyl)n+1
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.34 23
-
-
 Euglena gracilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.34 17 37 17°C: 80% of activity maximum, 30°C: 90% of activity maximum, 37°C: 80% of activity maximum Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.34 7.5
-
 glycylglycine buffer Euglena gracilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.34 7 7.8 7: about 60% of activity maximum, 7.8: about 70% of activity maximum Euglena gracilis