Literature summary extracted from
Skov, L.K.; Mirza, O.; Henriksen, A.; de Montalk, G.P.; Remaud-Simeon, M.; Sarcabal, P.; Willemot, R.M.; Monsan, P.; Gajhede, M.
Amylosucrase, a glucan-synthesizing enzyme from the alpha-amylase family (2001), J. Biol. Chem., 276, 25273-25278.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.4.1.4 |
- |
Neisseria polysaccharea |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.4.1.4 |
recombinant enzyme, equal amounts of 4 mg/ml enzyme in 150 mM NaCl, 50 mM Tris-HCl, pH 7.0, 1 mM EDTA and 1 mM dithiothreitol and reservoir solution consisting of 30% polyethylene glycol 6000 and 100 mM HEPES, pH 7.0, crystal structure at 1.4 A resolution |
Neisseria polysaccharea |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.4.1.4 |
Neisseria polysaccharea |
Q9ZEU2 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.4.1.4 |
- |
Neisseria polysaccharea |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.4.1.4 |
sucrose |
enzyme catalyzes both sucrose hydrolysis and oligosaccharide and polymer synthesis in the absence of an activator polymer |
Neisseria polysaccharea |
glucose + maltose + maltotriose + turanose + insoluble polymer |
with 10 mM sucrose as the sole substrate, 30% glucose, 29% maltose, 18% maltotriose, 11% turanose and 12% insoluble polymer respectively |
? |
|