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Literature summary extracted from

  • Basu, S.; Schultz, A.M.; Basu, M.; Roseman, S.
    Enzymatic synthesis of galactocerebroside by a galactosyltransferase from embryonic chicken brain (1971), J. Biol. Chem., 246, 4272-4279.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.47 freezing causes 80-85% loss of activity, 2-mercaptoethanol protects against inactivation by freezing Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.47 DL-sphingosine at higher concentrations Gallus gallus
2.4.1.47 DL-sphingosine
-
Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.47 0.04
-
UDPgalactose
-
Gallus gallus
2.4.1.47 0.11
-
2-(2-hydroxyacyl)sphingosine
-
Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.47 Ca2+ stimulates at 10 mM, dialyzed enzyme has requirement for divalent cations Gallus gallus
2.4.1.47 Mg2+ stimulates Gallus gallus
2.4.1.47 Mg2+ stimulates Rattus norvegicus
2.4.1.47 Mg2+ dialyzed enzyme has requirement for divalent cations, optimal stimulation at 5 mM Gallus gallus
2.4.1.47 Mn2+ stimulates Gallus gallus
2.4.1.47 Mn2+ stimulates Rattus norvegicus
2.4.1.47 Mn2+ at 10 mM, dialyzed enzyme has requirement for divalent cations Gallus gallus
2.4.1.47 additional information not activated by Cu2+, Fe2+, Co2+, Ni2+, Zn2+, Cd2+ Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.47 UDPgalactose + 2-(2-hydroxyacyl)sphingosine Gallus gallus i.e. 2-hydroxy fatty acid ceramide, synthesis of cerebroside 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
-
?
2.4.1.47 UDPgalactose + 2-(2-hydroxyacyl)sphingosine Gallus gallus enzyme may play an important role in the synthesis of at least one type of myelin 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.47 Cavia porcellus
-
3 days old
-
2.4.1.47 Gallus gallus
-
19-20 days old embryos
-
2.4.1.47 Rattus norvegicus
-
1 month old
-
2.4.1.47 Sus scrofa
-
fetus
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.47 brain
-
Gallus gallus
-
2.4.1.47 brain
-
Cavia porcellus
-
2.4.1.47 brain
-
Rattus norvegicus
-
2.4.1.47 brain
-
Sus scrofa
-
2.4.1.47 embryo maximal activity in 19-20 day-old embryos Gallus gallus
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.47 4°C, dialyzed enzyme, 0.1% 2-mercaptoethanol, 0.32 M sucrose, 7 days, stable Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.47 additional information
-
Rattus norvegicus ?
-
?
2.4.1.47 additional information not: galactose, galactose-1-phosphat, ADP-galactose Gallus gallus ?
-
?
2.4.1.47 UDPgalactose + 2-(2-hydroxyacyl)sphingosine
-
Rattus norvegicus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP i.e. 2-hydroxy fatty acid galactosylceramide ?
2.4.1.47 UDPgalactose + 2-(2-hydroxyacyl)sphingosine highly specific Gallus gallus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP product: galactocerebroside containing 2-hydroxy fatty acids linked to the amino group of the sphingosine moiety ?
2.4.1.47 UDPgalactose + 2-(2-hydroxyacyl)sphingosine ceramide containing 2-hydroxystearic acid as the predominant fatty acid Gallus gallus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP product: galactocerebroside containing 2-hydroxy fatty acids linked to the amino group of the sphingosine moiety ?
2.4.1.47 UDPgalactose + 2-(2-hydroxyacyl)sphingosine i.e. 2-hydroxy fatty acid ceramide, synthesis of cerebroside Gallus gallus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
-
?
2.4.1.47 UDPgalactose + 2-(2-hydroxyacyl)sphingosine enzyme may play an important role in the synthesis of at least one type of myelin Gallus gallus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
-
?
2.4.1.47 UDPglucose + 2-(2-hydroxyacyl)sphingosine at 7-10% of the activity with UDP-galactose Gallus gallus glucocerebroside + UDP
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.47 37
-
assay at Gallus gallus
2.4.1.47 37
-
assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.47 7.7
-
in bicine buffer Gallus gallus