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Literature summary extracted from

  • Sherwood, A.L.; Stroud, M.R.; Levery, S.B.; Holmes, E.H.
    An amino acid region at the N-terminus of rat hepatoma alpha1->2 fucosyltransferase modulates enzyme activity and interaction with lipids: strong preference for glycosphingolipids containing terminal Galbeta1->3GalNAc-structures (2001), Biochemistry, 40, 5708-5719.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.69 CHAPSO the truncated enzyme is optimally activated by CHAPSO, showing little activity in absence Rattus norvegicus
2.4.1.69 Triton CF-54 the native and the recombinant full-length enzyme display significant activity in absence of detergent or phospholipid and are optimally activated in presence of Triton CF-54 detergent Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.69 full length enzyme and truncated enzyme missing the first 27 amino acid residues from the N-terminus, comprising the cytoplasmic and transmembrane domains Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.69 additional information recombinant full length enzyme has a specific activity over 80fold higher than the truncated enzyme missing the first 27 amino acid residues from the N-terminus, comprising the cytoplasmic and transmembrane domains. The native and the recombinant full-length enzyme display significant activity in absence of detergent or phospholipid and are optimally activated in presence of Triton CF-54 detergent. The truncated enzyme is optimally activated by CHAPSO, showing littel activity in absence Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.69 Rattus norvegicus Q10984
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-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.69 H35 cell
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 Rattus norvegicus
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2.4.1.69 hepatoma cell e.g. hepatoma H35 cells, the enzyme is induced during early stages of chemical hepatocarcinogenesis in parenchymal cells of Fischer 344 rats fed a diet supplemented with 0.03% N-2-acetylaminofluorene, undetectable in normal liver tissue Rattus norvegicus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.69 GDP-L-fucose + beta-D-galactosyl-R
-
 Rattus norvegicus GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
 ?
2.4.1.69 additional information the enzyme shows a strong preference for terminal Galbeta1, 3GalNAc structures Rattus norvegicus ?
-
 ?