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Literature summary extracted from

  • Ishikura, N.; Yang, Z.Q.; Teramoto, S.
    UDP-D-glucose:flavonol 3-O- and 7-O-glucosyl transferases from young leaves of Paederia seandens var. mairei (1993), Z. Naturforsch. C, 48, 563-569.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.91 2-mercaptoethanol 14 mM, stimulates to 134% Paederia scandens
2.4.1.237 2-mercaptoethanol 14 mM, stimulates Paederia scandens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.91 Cu2+
-
 Paederia scandens
2.4.1.91 dithioerythritol
-
 Paederia scandens
2.4.1.91 iodoacetamide
-
 Paederia scandens
2.4.1.91 iodoacetate
-
 Paederia scandens
2.4.1.91 NEM
-
 Paederia scandens
2.4.1.91 PCMB
-
 Paederia scandens
2.4.1.91 Zn2+
-
 Paederia scandens
2.4.1.237 Ca2+ 10 mM, 41% inhibition Paederia scandens
2.4.1.237 Co2+ 1 mM, 95% inhibition Paederia scandens
2.4.1.237 Cu2+ 1 mM, 97% inhibition Paederia scandens
2.4.1.237 dithioerythritol 10 mM, 50% inhibition Paederia scandens
2.4.1.237 EDTA 10 mM, 58% inhibition Paederia scandens
2.4.1.237 iodoacetamide 1 mM, 26% inhibition Paederia scandens
2.4.1.237 iodoacetate 1 mM, 21% inhibition Paederia scandens
2.4.1.237 KCl 10 mM, 30% inhibition Paederia scandens
2.4.1.237 Mg2+ 10 mM, 64% inhibition Paederia scandens
2.4.1.237 Mn2+ 1 mM, 82% inhibition Paederia scandens
2.4.1.237 N-ethylmaleimide 1 mM, 94% inhibition, 14 mM 2-mercaptoethanol restores activity Paederia scandens
2.4.1.237 p-chloromercuribenzoate 1 mM, 91% inhibition, 14 mM 2-mercaptoethanol restores activity Paederia scandens
2.4.1.237 Phenylmercuriacetate 1 mM, 40% inhibition Paederia scandens
2.4.1.237 Zn2+ 1 mM, 98% inhibition Paederia scandens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.91 0.00114
-
 kaempferol
-
 Paederia scandens
2.4.1.91 0.0133
-
 UDPglucose
-
 Paederia scandens
2.4.1.237 0.00769
-
 kaempferol pH 7.5 Paederia scandens
2.4.1.237 0.01539
-
 UDP-glucose pH 7.5, cosubstrate kaempferol Paederia scandens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.237 additional information no requirement for Mg2+, Ca2+ or K+ Paederia scandens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.91 43000
-
 gel filtration Paederia scandens
2.4.1.237 43000
-
 gel filtration Paederia scandens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.237 UDP-glucose + a flavonol Paederia scandens plays a role in the glycosylation steps of flavonols UDP + a flavonol 7-O-beta-D-glucoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.91 Paederia scandens
-
 var. Mairei
-
2.4.1.237 Paederia scandens
-
 var. Mairei
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.91 partial Paederia scandens
2.4.1.237 135.5fold, partial Paederia scandens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.91 leaf young Paederia scandens
-
2.4.1.237 leaf young Paederia scandens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.91 additional information
-
-
 Paederia scandens
2.4.1.237 1.63
-
 pH 7.5 Paederia scandens

Storage Stability

EC Number Storage Stability Organism
2.4.1.91 -20°C, 20 mM Tris-HCl, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, 15% loss of activity after 2 days Paederia scandens
2.4.1.237 -20°C, partially purified enzyme, 20 mM Tris-HCl, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, 2 days: 15% loss of activity, 8 days: 60% loss of activity Paederia scandens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.91 UDP-alpha-D-glucose + isorhamnetin 71% of the activity with kaempferol Paederia scandens UDP + isorhamnetin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDPglucose + kaempferol
-
 Paederia scandens UDP + kaempferol 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + kaempferol 5,7,4'-trimethyl ether 4% of the activity with kaempferol Paederia scandens UDP + kaempferol 5,7,4'-trimethyl ether 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + myricetin 43% of the activity with kaempferol Paederia scandens UDP + myrecetin 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + quercetin 76% of the activity with kaempferol Paederia scandens UDP + quercetin 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + rhamnetin 70% of the activity with kaempferol Paederia scandens UDP + rhamnetin 3-O-glucoside
-
 ?
2.4.1.237 additional information not: rhamnetin, kaempferol 7-O-glucoside, quercetin 7-O-glucoside, ADP-D-glucose, GDP-D-glucose Paederia scandens ?
-
 ?
2.4.1.237 UDP-alpha-D-glucose + kaempferol best substrate Paederia scandens UDP + kaempferol 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-alpha-D-glucose + quercetin 45% of the activity with kaempferol as substrate Paederia scandens UDP + quercetin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + a flavonol plays a role in the glycosylation steps of flavonols Paederia scandens UDP + a flavonol 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + a flavonol F 7 GT mediates the transfer of D-glucose exclusively to the 7-hydroxyl group of kaempferol and some flavonols, highly specific for UDP-D-glucose Paederia scandens UDP + a flavonol 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + caffeic acid 14% of the activity with kaempferol as substrate Paederia scandens UDP + caffeic acid 4-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + hydroquinone 10% of the activity with kaempferol as substrate Paederia scandens UDP + hydroquinone O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + isorhamnetin 88% of the activity with kaempferol as substrate Paederia scandens UDP + isorhamnetin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + kaempferol 3,4'-dimethylether 30% of the activity with kaempferol as substrate Paederia scandens UDP + kaempferol 3,4'-dimethylether 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + kaempferol 3-O-glucoside astragalin, 17% of the activity with kaempferol as substrate Paederia scandens UDP + kaempferol 3,7-O-beta-D-diglucoside
-
 ?
2.4.1.237 UDP-glucose + kaempferol 3-O-rhamnosylglucoside 11% of the activity with kaempferol as substrate Paederia scandens UDP + kaempferol 3-O-rhamnosylglucoside 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + kaempferol 5,7,4'-trimethylether 9% of the activity with kaempferol as substrate Paederia scandens UDP + kaempferol 5,7,4'-trimethylether 3-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + myricetin 26% of the activity with kaempferol as substrate Paederia scandens UDP + myricetin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + p-coumaric acid 9% of the activity with kaempferol as substrate Paederia scandens UDP + (2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoic acid
-
 ?
2.4.1.237 UDP-glucose + quercetin 3-O-glucoside isoquercetin, 32% of the activity with kaempferol as substrate Paederia scandens UDP + quercetin 3,7-O-beta-D-diglucoside
-
 ?
2.4.1.237 UDP-glucose + rutin 21% of the activity with kaempferol as substrate Paederia scandens UDP + rutin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + scopoletin 8% of the activity with kaempferol as substrate Paederia scandens UDP + scopoletin 7-O-beta-D-glucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.237 F 7 GT
-
 Paederia scandens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.91 7.5
-
 Tris-HCl buffer Paederia scandens
2.4.1.91 8
-
 imidazole-HCl buffer Paederia scandens
2.4.1.237 7.5
-
 Tris-HCl and histidine-HCl buffer Paederia scandens
2.4.1.237 8
-
 imidazole-HCl buffer Paederia scandens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.237 Paederia scandens chromatofocusing
-
 4.5