EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.99 | pyridoxal-HCl | 20 mM, stimulation to 147% of the control | Helianthus tuberosus | |
2.4.1.99 | pyridoxine | 20 mM, stimulation to 120% of the control | Helianthus tuberosus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.99 | Ag+ | 1 mM, 15% inhibition | Helianthus tuberosus | |
2.4.1.99 | Cu2+ | 1 mM CuSO4, 47% inhibition | Helianthus tuberosus | |
2.4.1.99 | iodoacetamide | 1 mM, 10% inhibition | Helianthus tuberosus | |
2.4.1.99 | Mn2+ | 5 mM 20% inhibition | Helianthus tuberosus | |
2.4.1.99 | NEM | 1 mM, 10% inhibition | Helianthus tuberosus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.99 | 67000 | - |
gel filtration | Helianthus tuberosus |
2.4.1.99 | 90000 | - |
non-denaturing PAGE | Helianthus tuberosus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.99 | sucrose + sucrose | Helianthus tuberosus | the enzyme is involved in fructan biosynthesis | D-glucose + 1F-beta-D-fructosylsucrose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.99 | Helianthus tuberosus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.99 | tuber | - |
Helianthus tuberosus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.99 | additional information | - |
- |
Helianthus tuberosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.99 | 1,1-nystose + 1,1-nystose | self-transfructosylation at a low rate | Helianthus tuberosus | 1,1,1-fructosylnystose + 1-kestose | - |
? | |
2.4.1.99 | 1-kestose + 1-kestose | self-transfructosylation at a low rate | Helianthus tuberosus | 1,1-nystose + sucrose | - |
? | |
2.4.1.99 | additional information | the enzyme also catalyzes the removal of the terminal fructosyl unit from both 1-kestose and 1,1-nystose, which results in the release of sucrose and 1-kestose, respectively and free fructose. The enzyme has no hydrolytic activity against sucrose | Helianthus tuberosus | ? | - |
? | |
2.4.1.99 | sucrose + sucrose | - |
Helianthus tuberosus | D-glucose + 1F-beta-D-fructosylsucrose | - |
? | |
2.4.1.99 | sucrose + sucrose | the enzyme is involved in fructan biosynthesis | Helianthus tuberosus | D-glucose + 1F-beta-D-fructosylsucrose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.99 | More | electrophoresis or gel filtration under denaturing conditions yields a 27000 Da and a 55000 Da fragment | Helianthus tuberosus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.99 | 20 | 25 | synthesis of 1-kestose | Helianthus tuberosus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.99 | 5 | 37 | 50% of maximal activity at 5°C and at 37°C, synthesis of 1-kestose | Helianthus tuberosus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.99 | 3.5 | 5 | - |
Helianthus tuberosus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.99 | 2.7 | 6.5 | about 50% of maximal activity at pH 2.7 and at pH 6.5 | Helianthus tuberosus |