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Literature summary extracted from

  • Barsacchi, D.; Cappiello, M.; Tozzi, M.G.; Del Corso, A.; Peccatori, M.; Camici, M.; Ipata, P.L.; Mura, U.
    Purine nucleoside phosphorylase from bovine lens: purification and properties (1992), Biochim. Biophys. Acta, 1160, 163-170.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.1 freezing and thawing results in complete loss of activity of the pure enzyme Bos taurus
2.4.2.1 photooxidation in presence of methylene blue is pH-dependent Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 alpha-D-ribose 1-phosphate
-
 Bos taurus
2.4.2.1 folate 0.05-0.1 mM, slight competitive inhibitor Bos taurus
2.4.2.1 guanine
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.014
-
 guanine reaction with ribose 1-phosphate Bos taurus
2.4.2.1 0.017
-
 guanine reaction with deoxyribose 1-phosphate Bos taurus
2.4.2.1 0.02
-
 hypoxanthine with ribose 1-phosphate Bos taurus
2.4.2.1 0.021
-
 hypoxanthine reaction with deoxyribose 1-phosphate Bos taurus
2.4.2.1 0.024
-
 2'-deoxyguanosine
-
 Bos taurus
2.4.2.1 0.028
-
 phosphate reaction with inosine Bos taurus
2.4.2.1 0.029
-
 guanosine
-
 Bos taurus
2.4.2.1 0.036
-
 Inosine
-
 Bos taurus
2.4.2.1 0.041
-
 alpha-D-ribose 1-phosphate reaction with guanine Bos taurus
2.4.2.1 0.042
-
 alpha-D-ribose 1-phosphate reaction with hypoxanthine Bos taurus
2.4.2.1 0.043
-
 2'-deoxyinosine
-
 Bos taurus
2.4.2.1 0.129
-
 alpha-D-deoxyribose 1-phosphate reaction with hypoxanthine Bos taurus
2.4.2.1 0.133
-
 alpha-D-deoxyribose 1-phosphate reaction with guanine Bos taurus
2.4.2.1 0.45
-
 Xanthosine
-
 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.1 purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate rapid equilibrium random bi-bi mechanism with formation of abortive complexes Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.1 ocular lens
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 77.5
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.4.2.1 4°C, 10 mM sodium phosphate buffer, pH 7.0, stable for at least 4 months provided a weekly dialysis with 2 mM DTT is performed Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 deoxyguanosine + phosphate
-
 Bos taurus guanine + alpha-D-deoxyribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate
-
 Bos taurus guanine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
 Bos taurus deoxyinosine + phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Bos taurus hypoxanthine + alpha-D-ribose 1-phosphate
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 4
-
 Xanthosine
-
 Bos taurus
2.4.2.1 45
-
 2'-deoxyguanosine
-
 Bos taurus
2.4.2.1 59
-
 guanosine
-
 Bos taurus
2.4.2.1 85
-
 2'-deoxyinosine
-
 Bos taurus
2.4.2.1 100
-
 alpha-D-ribose 1-phosphate reaction with hypoxanthine Bos taurus
2.4.2.1 103
-
 hypoxanthine reaction with ribose 1-phosphate Bos taurus
2.4.2.1 115
-
 alpha-D-deoxyribose 1-phosphate reaction with hypoxanthine Bos taurus
2.4.2.1 119
-
 Inosine
-
 Bos taurus
2.4.2.1 120
-
 alpha-D-ribose 1-phosphate reaction with guanine Bos taurus
2.4.2.1 127
-
 guanine reaction with ribose 1-phosphate Bos taurus
2.4.2.1 127
-
 hypoxanthine reaction with deoxyribose 1-phosphate Bos taurus
2.4.2.1 145
-
 alpha-D-deoxyribose 1-phosphate reaction with guanine Bos taurus
2.4.2.1 158
-
 guanine reaction with deoxyribose 1-phosphate Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 5.5 8
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.1 0.0065
-
 guanine with guanosine as variable substrate Bos taurus
2.4.2.1 0.0085
-
 alpha-D-ribose 1-phosphate with guanosine as variable substrate Bos taurus
2.4.2.1 0.025
-
 guanine with phosphate as variable substrate Bos taurus
2.4.2.1 0.035
-
 alpha-D-ribose 1-phosphate with phosphate as variable substrate Bos taurus