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Literature summary extracted from

  • Lewis, A.S.; Glantz, M.D.
    Bovine brain purine-nucleoside phosphorylase purification, characterization, and catalytic mechanism (1976), Biochemistry, 15, 4451-4457.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.1 strong susceptibility to photooxidation in presence of methylene blue. Maximal photoinactivation at pH 8.0, 85% loss of activity after only 1 min of irradiation Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 alpha-D-ribose 1-phosphate
-
 Bos taurus
2.4.2.1 guanine
-
 Bos taurus
2.4.2.1 guanosine
-
 Bos taurus
2.4.2.1 hypoxanthine
-
 Bos taurus
2.4.2.1 PCMB
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.013
-
 Inosine
-
 Bos taurus
2.4.2.1 0.03
-
 guanine
-
 Bos taurus
2.4.2.1 0.0435
-
 guanosine
-
 Bos taurus
2.4.2.1 21.5
-
 phosphate
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 38000
-
 2 * 38000, SDS-PAGE Bos taurus
2.4.2.1 78000 80000 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.1 purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate Theorell-Chance mechanism Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.1 brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 78.3
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 guanosine + phosphate
-
 Bos taurus guanine + alpha-D-ribose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.1 dimer 2 * 38000, SDS-PAGE Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 55
-
 rapid inactivation Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.1 0.012
-
 guanine with guanosine as variable substrate and 200 mM phosphate Bos taurus
2.4.2.1 0.0145
-
 guanine with guanosine as variable substrate and 40 mM phosphate Bos taurus
2.4.2.1 0.0313
-
 guanosine with guanine as variable substrate and 0.4 mM ribose 1-phosphate Bos taurus
2.4.2.1 0.05
-
 hypoxanthine with guanosine as variable substrate and 200 mM phosphate Bos taurus
2.4.2.1 0.065
-
 guanine with phosphate as variable substrate and 0.2 mM guanosine Bos taurus
2.4.2.1 0.32
-
 alpha-D-ribose 1-phosphate with phosphate as variable substrate and 0.2 mM guanosine Bos taurus
2.4.2.1 0.36
-
 alpha-D-ribose 1-phosphate with guanosine as variable substrate and 20 mM phosphate Bos taurus