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Literature summary extracted from

  • Wielgus-Kutrowska, B.; Tebbe, J.; Schroder, W.; Luic, M.; Shugar, D.; Saenger, W.; Koellner, G.; Bzowska, A.
    Cellulomonas sp. purine nucleoside phosphorylase (PNP): comparison with human and E. coli enzymes (1998), Adv. Exp. Med. Biol., 431, 259-264.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.1
-
 Cellulomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 8-bromo-N(9)-acycloguanosine
-
 Cellulomonas sp.
2.4.2.1 acyclovir
-
 Cellulomonas sp.
2.4.2.1 adenosine
-
 Cellulomonas sp.
2.4.2.1 Formycin A
-
 Cellulomonas sp.
2.4.2.1 formycin B
-
 Cellulomonas sp.
2.4.2.1 guanine
-
 Cellulomonas sp.
2.4.2.1 N(7)-acycloguanosine
-
 Cellulomonas sp.
2.4.2.1 N(9)-acycloguanosine
-
 Cellulomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.043
-
 Inosine
-
 Cellulomonas sp.
2.4.2.1 0.11
-
 guanosine
-
 Cellulomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 29000
-
 4 * 29000, MALDI-MS Cellulomonas sp.
2.4.2.1 112000
-
 gel filtration Cellulomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Cellulomonas sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 adenosine + phosphate no activity Cellulomonas sp. adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate
-
 Cellulomonas sp. guanine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Cellulomonas sp. hypoxanthine + alpha-D-ribose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.1 tetramer 4 * 29000, MALDI-MS Cellulomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 50
-
 stable up to Cellulomonas sp.
2.4.2.1 62
-
 pH 7.4, 50% inactivation Cellulomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.2.1 6.5
-
 rather unstable below Cellulomonas sp.
2.4.2.1 8.5 10 stable in presence of orthophosphate Cellulomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.1 0.0034
-
 N(9)-acycloguanosine
-
 Cellulomonas sp.
2.4.2.1 0.0036
-
 guanine
-
 Cellulomonas sp.
2.4.2.1 0.0041
-
 8-bromo-N(9)-acycloguanosine
-
 Cellulomonas sp.
2.4.2.1 0.019
-
 formycin B
-
 Cellulomonas sp.
2.4.2.1 0.062
-
 Formycin A
-
 Cellulomonas sp.
2.4.2.1 0.097
-
 N(7)-acycloguanosine
-
 Cellulomonas sp.
2.4.2.1 0.11
-
 acyclovir
-
 Cellulomonas sp.
2.4.2.1 0.16
-
 adenosine
-
 Cellulomonas sp.