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Literature summary extracted from

  • Shimosaka, M.; Fukuda, Y.; Murata, K.; Kimura, A.
    Purification and properties of orotate phosphoribosyltransferases from Escherichia coli K-12, and its derivative purine-sensitive mutant (1985), J. Biochem., 98, 1689-1697.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.10 adenine not inhibitory Escherichia coli
2.4.2.10 adenosine not inhibitory Escherichia coli
2.4.2.10 ADP not inhibitory Escherichia coli
2.4.2.10 AMP not inhibitory Escherichia coli
2.4.2.10 ATP not inhibitory Escherichia coli
2.4.2.10 HgCl2 slight inhibition of mutant, not wild-type Escherichia coli
2.4.2.10 iodoacetamide slight inhibition of mutant, not wild-type Escherichia coli
2.4.2.10 NEM slight inhibition of mutant, not wild-type Escherichia coli
2.4.2.10 PCMB reversible by DTT Escherichia coli
2.4.2.10 UTP not inhibitory Escherichia coli
2.4.2.10 Zn2+
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.10 0.0031 0.0036 orotidine 5'-phosphate wild-type Escherichia coli
2.4.2.10 0.013
-
 diphosphate wild-type Escherichia coli
2.4.2.10 0.03
-
 orotidine 5'-monophosphate
-
 Escherichia coli
2.4.2.10 0.03 0.035 Orotate wild-type Escherichia coli
2.4.2.10 0.038 0.04 5-phospho-alpha-D-ribose 1-diphosphate wild-type Escherichia coli
2.4.2.10 0.22 0.25 diphosphate
-
 Escherichia coli
2.4.2.10 0.36
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant Escherichia coli
2.4.2.10 0.44
-
 Orotate mutant Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.10 Ba2+ slight activation Escherichia coli
2.4.2.10 Ca2+ activation, 34% as effective as Mn2+ or Mg2+ Escherichia coli
2.4.2.10 Co2+ slight activation Escherichia coli
2.4.2.10 Mg2+ requirement Escherichia coli
2.4.2.10 Mn2+ can replace Mg2+ Escherichia coli
2.4.2.10 Mn2+ activation Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.10 23500
-
 2 * 23500, SDS-PAGE Escherichia coli
2.4.2.10 47000
-
 K-12, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.10 orotate + 5-phospho-alpha-D-ribose 1-diphosphate Escherichia coli involved in de novo synthesis of pyrimidine nucleotides orotidine 5'-phosphate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.10 Escherichia coli
-
 K-12, overproducing strains C-600(pNE24) (wild-type) and its purine-sensitive mutant PS100(pNE31), both harbouring hybrid multi-copy plasmids
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.10 OMP-agarose affinity chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.10 additional information
-
-
 Escherichia coli
2.4.2.10 0.41
-
 mutant Escherichia coli
2.4.2.10 15.2
-
 wild-type Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.10 orotate + 5-phospho-alpha-D-ribose 1-diphosphate reverse reaction: tri-, tetrapoly- or trimetaphosphate can replace diphosphate with 29%, 70% or 78% efficiency Escherichia coli orotidine 5'-phosphate + diphosphate
-
 r
2.4.2.10 orotate + 5-phospho-alpha-D-ribose 1-diphosphate involved in de novo synthesis of pyrimidine nucleotides Escherichia coli orotidine 5'-phosphate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.10 dimer 2 * 23500, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.10 8.5
-
 mutant Escherichia coli
2.4.2.10 9.5
-
 wild-type Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.10 7.2 9.5 about half-maximal activity at pH 7.2 and 9.5, mutant Escherichia coli
2.4.2.10 8.5 10.5 about half-maximal activity at pH 8.5 and about 70% of maximal activity at pH 10.5, wild-type Escherichia coli