Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bayles, D.O.; Fennington, G.J., Jr.; Hughes, T.A.
    Sequence and phylogenetic analysis of the Rhizobium leguminosarum biovar trifolii pyrE gene, overproduction, purification and characterization of orotate phosphoribosyltransferase (1997), Gene, 195, 329-336.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.10 structural gene pyrE Rhizobium leguminosarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.10 Orotate above 0.4 mM Rhizobium leguminosarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.10 0.0276
-
 Orotate
-
 Rhizobium leguminosarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.10 24700
-
 x * 24700, SDS-PAGE, recombinant enzyme Rhizobium leguminosarum

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.10 Rhizobium leguminosarum
-
 biovar trifolium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.10 recombinant enzyme Rhizobium leguminosarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.10 61
-
-
 Rhizobium leguminosarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.10 orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Rhizobium leguminosarum orotidine 5'-phosphate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.10 ? x * 24700, SDS-PAGE, recombinant enzyme Rhizobium leguminosarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.10 10
-
 recombinant enzyme Rhizobium leguminosarum