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Literature summary extracted from

  • Hayakawa, T.; Perkins, J.P.; Walsh, D.A.; Krebs, E.G.
    Physiochemical properties of rabbit skeletal muscle phosphorylase kinase (1973), Biochemistry, 12, 567-573.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.11.19 isoelectric focusing inactivates Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.19 DTNB only gradual loss of activity after more than 10 min, pH-dependent Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.19 Mg2+ requirement Oryctolagus cuniculus
2.7.11.19 Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Oryctolagus cuniculus
2.7.11.19 phosphate requirement, phosphate containing enzyme Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.19 additional information
-
 amino acid composition Oryctolagus cuniculus
2.7.11.19 additional information
-
 enzyme aggregates to high polymeric forms which arise as artifacts during isolation procedure due to sensitivity to high hydrostatic pressure, e.g. during sucrose density gradient centrifugation at very high angular velocities Oryctolagus cuniculus
2.7.11.19 1330000
-
 analytical ultracentrifugation Oryctolagus cuniculus
2.7.11.19 1330000
-
 nonactivated enzyme Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.19 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.19
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.19 skeletal muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.19 ATP + phosphorylase b cosubstrate: Mg-ATP complex Oryctolagus cuniculus ADP + phosphorylase a
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.19 additional information
-
 pI: 5.77 (nonactivated rabbit enzyme) Oryctolagus cuniculus
2.7.11.19 additional information
-
 nonactivated kinase activity ratios at pH 6.8/8.2: between 0.01-0.05, activated kinase activity ratio: about 0.6 Oryctolagus cuniculus