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Literature summary extracted from

  • Fall, R.R.; West, C.A.
    Purification and properties of kaurene synthetase from Fusarium moniliforme (1971), J. Biol. Chem., 246, 6913-6928.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.19 2-mercaptoethanol
-
 Fusarium verticillioides
4.2.3.19 cysteine
-
 Fusarium verticillioides
4.2.3.19 dithiothreitol stimulates activity in presence of EDTA Fusarium verticillioides

General Stability

EC Number General Stability Organism
4.2.3.19 sensitive on freezing and thawing Fusarium verticillioides

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.19 2-(N,N-dimethyl-N-octylammonium bromide)-p-methan-1-ol Q-64 Fusarium verticillioides
4.2.3.19 copalyl diphosphate slight substrate inhibition at concentrations above 0.0007 mM Fusarium verticillioides
4.2.3.19 Cu2+
-
 Fusarium verticillioides
4.2.3.19 deoxycholate
-
 Fusarium verticillioides
4.2.3.19 EDTA
-
 Fusarium verticillioides
4.2.3.19 Hg2+
-
 Fusarium verticillioides
4.2.3.19 iodacetamide
-
 Fusarium verticillioides
4.2.3.19 N,N-dimethylaminoethyl 2,2-diphenylphenylether SKF 3301A Fusarium verticillioides
4.2.3.19 N-ethylmaleimide
-
 Fusarium verticillioides
4.2.3.19 p-hydroxymercuribenzoate
-
 Fusarium verticillioides
4.2.3.19 Phosphone D
-
 Fusarium verticillioides
4.2.3.19 Phosphone S
-
 Fusarium verticillioides
4.2.3.19 sulfhydryl groups
-
 Fusarium verticillioides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.19 0.001
-
 copalyl diphosphate
-
 Fusarium verticillioides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.19 Ca2+ no effect Fusarium verticillioides
4.2.3.19 Co2+ can partially replace Mg2+ Fusarium verticillioides
4.2.3.19 Mg2+ required, optimum concentration 5 mM Fusarium verticillioides
4.2.3.19 Mn2+ can partially replace Mg2+ Fusarium verticillioides
4.2.3.19 Ni2+ can partially replace Mg2+ Fusarium verticillioides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.19 430000
-
 sucrose density gradient centrifugation, crude enzyme Fusarium verticillioides
4.2.3.19 450000
-
 sucrose density gradient centrifugation, purified enzyme Fusarium verticillioides
4.2.3.19 490000
-
 gel filtration Fusarium verticillioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.19 ent-copalyl diphosphate Fusarium verticillioides involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.19 Fusarium verticillioides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.19 partial, copurified with ent-kaurene synthase A (EC 5.5.1.13) activity Fusarium verticillioides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.19 0.123
-
 purified enzyme Fusarium verticillioides

Storage Stability

EC Number Storage Stability Organism
4.2.3.19 -20°C, 25% glycerol, several months, no loss of activity for purified enzyme Fusarium verticillioides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.19 copalyl diphosphate
-
 Fusarium verticillioides sandaracopimaradiene + diphosphate
-
 ?
4.2.3.19 ent-copalyl diphosphate
-
 Fusarium verticillioides ent-kaurene + diphosphate
-
 ?
4.2.3.19 ent-copalyl diphosphate involved in biosynthesis of gibberellins Fusarium verticillioides ent-kaurene + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.19 More part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Fusarium verticillioides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.19 6.9
-
 TES or HEPES buffer Fusarium verticillioides
4.2.3.19 7.5
-
 phosphate buffer Fusarium verticillioides