EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.3.19 | expressed in Escherichia coli JM109 as glutathione-S-transferase fusion protein, enzyme shows EC 4.2.3.19 and EC 5.5.1.13 activity | Phaeosphaeria sp. |
5.5.1.13 | expression in Escherichia coli of a recombinant GST fused enzyme | Phaeosphaeria sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.3.19 | 2'-isopropyl-4'(trimethylammonium chloride)-5'-methylphenyl piperidine-1-carboxylate | Amo-1618 | Phaeosphaeria sp. | |
4.2.3.19 | EDTA | - |
Phaeosphaeria sp. | |
5.5.1.13 | 2'-isopropyl-4'-(trimethylammonium chloride)-5'-methylphenyl-piperidine-1-carboxylate | AMO-1618, a quaternary ammonium salt, leads to total enzyme inhibition at 0.001 mM | Phaeosphaeria sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.3.19 | Mg2+ | required | Phaeosphaeria sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.3.19 | 132000 | - |
SDS-PAGE, cloned fusion protein | Phaeosphaeria sp. |
5.5.1.13 | 106000 | - |
calculated from amino acid sequence | Phaeosphaeria sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.19 | ent-copalyl diphosphate | Phaeosphaeria sp. | involved in biosynthesis of gibberellins | ent-kaurene + diphosphate | - |
? | |
4.2.3.19 | ent-copalyl diphosphate | Phaeosphaeria sp. L487 | involved in biosynthesis of gibberellins | ent-kaurene + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.19 | Phaeosphaeria sp. | O13284 | - |
- |
4.2.3.19 | Phaeosphaeria sp. L487 | O13284 | - |
- |
5.5.1.13 | Phaeosphaeria sp. | O13284 | from mycelia | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.5.1.13 | recombinant enzyme, using Sepharose 4B glutathione affinity resin and SDS PAGE electrophoresis | Phaeosphaeria sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.19 | ent-copalyl diphosphate | - |
Phaeosphaeria sp. | ent-kaurene + diphosphate | - |
? | |
4.2.3.19 | ent-copalyl diphosphate | involved in biosynthesis of gibberellins | Phaeosphaeria sp. | ent-kaurene + diphosphate | - |
? | |
4.2.3.19 | ent-copalyl diphosphate | - |
Phaeosphaeria sp. L487 | ent-kaurene + diphosphate | - |
? | |
4.2.3.19 | ent-copalyl diphosphate | involved in biosynthesis of gibberellins | Phaeosphaeria sp. L487 | ent-kaurene + diphosphate | - |
? | |
5.5.1.13 | copalyl diphosphate | bifunctional enzyme | Phaeosphaeria sp. | ent-kaurene + diphosphate | - |
? | |
5.5.1.13 | geranylgeranyl diphosphate | - |
Phaeosphaeria sp. | copalyl diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.3.19 | More | part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) | Phaeosphaeria sp. |