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Literature summary extracted from

  • Aach, H.; Boese, G.; Graebe, J.E.
    ent-Kaurene biosynthesis in a cell-free system from wheat (Triticum aestivum L.) seedlings and the localization of ent-kaurene synthetase in plastids of three species (1995), Planta, 197, 333-342.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.19 plastid
-
 Pisum sativum 9536
-
4.2.3.19 plastid
-
 Cucurbita maxima 9536
-
4.2.3.19 plastid probably in stroma, no activity in leaf chloroplasts Triticum aestivum 9536
-
5.5.1.13 chloroplast stroma
-
 Triticum aestivum 9570
-
5.5.1.13 chloroplast stroma
-
 Pisum sativum 9570
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.19 ent-copalyl diphosphate Triticum aestivum involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
 ?
4.2.3.19 ent-copalyl diphosphate Cucurbita maxima involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
 ?
4.2.3.19 ent-copalyl diphosphate Pisum sativum involved in biosynthesis of gibberellins ent-kaurene + diphosphate additionally an unidentified acid labile compound is formed that is not found in products from pea and pumpkin ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.19 Cucurbita maxima
-
-
-
4.2.3.19 Pisum sativum
-
-
-
4.2.3.19 Triticum aestivum
-
-
-
5.5.1.13 Cucurbita maxima
-
 pumpkin
-
5.5.1.13 Pisum sativum
-
 pea, Torsdag cultivar
-
5.5.1.13 Triticum aestivum
-
 wheat, Jubilar cultivar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.13 partial, gradient centrifugation Cucurbita maxima
5.5.1.13 partial, using ammonium sulfate fractionation, column chromatography on Sephadex G25 Triticum aestivum
5.5.1.13 partial, using ammonium sulfate fractionation, column chromatography on Sephadex G25 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.19 seedling shoots of seedlings Triticum aestivum
-
5.5.1.13 endosperm
-
 Cucurbita maxima
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.19 ent-copalyl diphosphate
-
 Triticum aestivum ent-kaurene + diphosphate
-
 ?
4.2.3.19 ent-copalyl diphosphate
-
 Cucurbita maxima ent-kaurene + diphosphate
-
 ?
4.2.3.19 ent-copalyl diphosphate
-
 Pisum sativum ent-kaurene + diphosphate additionally an unidentified acid labile compound is formed that is not found in products from pea and pumpkin ?
4.2.3.19 ent-copalyl diphosphate involved in biosynthesis of gibberellins Triticum aestivum ent-kaurene + diphosphate
-
 ?
4.2.3.19 ent-copalyl diphosphate involved in biosynthesis of gibberellins Cucurbita maxima ent-kaurene + diphosphate
-
 ?
4.2.3.19 ent-copalyl diphosphate involved in biosynthesis of gibberellins Pisum sativum ent-kaurene + diphosphate additionally an unidentified acid labile compound is formed that is not found in products from pea and pumpkin ?
5.5.1.13 geranylgeranyl diphosphate
-
 Triticum aestivum ent-kaurene + ?
-
 ?
5.5.1.13 geranylgeranyl diphosphate
-
 Pisum sativum ent-kaurene + ?
-
 ?
5.5.1.13 geranylgeranyl diphosphate
-
 Cucurbita maxima ent-kaurene + ? gives ent-kaurene + an unidentified compound ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.19 More part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Triticum aestivum
4.2.3.19 More part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Pisum sativum
4.2.3.19 More part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Cucurbita maxima