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Literature summary extracted from

  • Jensen, S.E.; Westlake, D.W.S.; Wolfe, S.
    Partial purification and characterization of isopenicillin N epimerase activity from Streptomyces clavuligerus (1983), Can. J. Microbiol., 29, 1526-1531.
    View publication on PubMed

General Stability

EC Number General Stability Organism
5.1.1.17 unstable enzyme Streptomyces clavuligerus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.17 hydroxylamine 10 mM, 68% inhibition, readdition of pyridoxal 5-phosphate causes an increase in activity to 63% of the control Streptomyces clavuligerus
5.1.1.17 Iproniazid 10 mM, 33% inhibition Streptomyces clavuligerus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.17 50000
-
-
 Streptomyces clavuligerus
5.1.1.17 60000
-
 gel filtration Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.17 isopenicillin N Streptomyces clavuligerus
-
 penicillin N
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.17 Streptomyces clavuligerus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.17 ammonium sulfate, Sephadex G-200, DEAE-trisacryl Streptomyces clavuligerus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.17 additional information
-
 19.53 units, 1 unit is defined as the amount of enzyme which produces 0.001 mg of penicillin N in 1 h Streptomyces clavuligerus

Storage Stability

EC Number Storage Stability Organism
5.1.1.17 -65°C, 1 week, 61% loss of activity Streptomyces clavuligerus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.17 isopenicillin
-
 Streptomyces clavuligerus penicillin N
-
 ?
5.1.1.17 isopenicillin N
-
 Streptomyces clavuligerus penicillin N
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.1.17 monomer 1 * 60000, SDS-PAGE Streptomyces clavuligerus

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.17 pyridoxal 5'-phosphate putative cofactor, does not increase enzyme activity in assay system, stabilizes enzyme during purification Streptomyces clavuligerus