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Literature summary extracted from

  • Usui, S.; Yu, C.A.
    Purification and properties of isopenicillin-N epimerase from Streptomyces clavuligerus (1989), Biochim. Biophys. Acta, 999, 78-85.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.17 4-chloromercuribenzoate 1 mM, 88% inhibition Streptomyces clavuligerus
5.1.1.17 D-cycloserine 1 mM, 23% inhibition Streptomyces clavuligerus
5.1.1.17 FAD 0.5 mM, 80% inhibition Streptomyces clavuligerus
5.1.1.17 fluorescamine inactivation at molar ratios higher than 2 Streptomyces clavuligerus
5.1.1.17 hydroxylamine 1 mM, 95% inhibition Streptomyces clavuligerus
5.1.1.17 iodoacetamide 1 mM, 28% inhibition Streptomyces clavuligerus
5.1.1.17 Iproniazid 1 mM, 25% inhibition Streptomyces clavuligerus
5.1.1.17 isoniazid 1 mM, 23% inhibition Streptomyces clavuligerus
5.1.1.17 N-ethylmaleimide 1 mM, 45% inhibition Streptomyces clavuligerus
5.1.1.17 Phenylglyoxal 1 mM, 33% inhibition Streptomyces clavuligerus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.17 0.0024
-
 pyridoxal 5'-phosphate
-
 Streptomyces clavuligerus
5.1.1.17 0.3
-
 isopenicillin N
-
 Streptomyces clavuligerus
5.1.1.17 0.78
-
 Penicillin N
-
 Streptomyces clavuligerus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.17 47000
-
 1 * 47000, SDS-PAGE Streptomyces clavuligerus
5.1.1.17 50000
-
 gel filtration Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.17 isopenicillin N Streptomyces clavuligerus
-
 penicillin N
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.17 Streptomyces clavuligerus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.17 ammonium sulfate, DE-52, DEAE-Affi-gel blue, Sephadex G-200, calcium phosphate, Mono Q Streptomyces clavuligerus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.17 3.85
-
 isopenicillin N epimerization Streptomyces clavuligerus
5.1.1.17 7.77
-
 penicillin N epimerization Streptomyces clavuligerus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.17 deacetoxycephalosporin C less than 1% of activity with penicillin N Streptomyces clavuligerus ?
-
 ?
5.1.1.17 isopenicillin
-
 Streptomyces clavuligerus penicillin N
-
 r
5.1.1.17 isopenicillin N
-
 Streptomyces clavuligerus penicillin N
-
 r

Subunits

EC Number Subunits Comment Organism
5.1.1.17 monomer 1 * 47000, SDS-PAGE Streptomyces clavuligerus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.1.17 45
-
 rapid denaturation above Streptomyces clavuligerus
5.1.1.17 60
-
 complete inactivation after 10 min Streptomyces clavuligerus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.17 7.8 8.3
-
 Streptomyces clavuligerus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.1.17 5.3 10 stable at 4°C for 24 h Streptomyces clavuligerus

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.17 pyridoxal 5'-phosphate 1 mol of pyridoxal 5'-phosphate per mol enzyme, 1.5fold increase in activity in the presence of 0.01 mM pyridoxal 5'-phosphate Streptomyces clavuligerus