Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Feingold, D.S.; Hoffee, P.A.
    Other deoxy sugar aldolases (1972), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 7, 303-321.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.2.19 additional information the enzyme contains 12 sulfhydryl groups per molecule Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.17 EDTA
-
 Escherichia coli
4.1.2.19 1,10-phenanthroline competitive with L-rhamnulose 1-phosphate or glycerone phosphate; noncompetitive with L-lactaldehyde Escherichia coli
4.1.2.19 2,2'-dipyridyl
-
 Escherichia coli
4.1.2.19 8-hydroxyquinoline 5-sulfonic acid
-
 Escherichia coli
4.1.2.19 EDTA
-
 Escherichia coli
4.1.2.19 Li+
-
 Escherichia coli
4.1.2.19 NaBH4
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.17 0.7
-
 L-Fuculose 1-phosphate
-
 Escherichia coli
4.1.2.19 0.2
-
 glycerone phosphate
-
 Escherichia coli
4.1.2.19 0.2
-
 L-rhamnulose 1-phosphate
-
 Escherichia coli
4.1.2.19 1
-
 L-lactaldehyde
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.17 Ca2+ divalent cation required Escherichia coli
4.1.2.17 Mg2+ divalent cation required Escherichia coli
4.1.2.17 Mg2+ 0.01 M, restores activity after dialysis with EDTA Escherichia coli
4.1.2.17 Mn2+ divalent cation required Escherichia coli
4.1.2.17 Mn2+ 0.01 M, restores activity after dialysis with EDTA Escherichia coli
4.1.2.19 Co2+ restores activity of the enzyme after inactivation by dialysis against chelating agents or by treatment with SDS and 2-mercaptoethanol Escherichia coli
4.1.2.19 Cs+ Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity Escherichia coli
4.1.2.19 K+ Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity Escherichia coli
4.1.2.19 Mg2+ restores activity of the enzyme after inactivation by dialysis against chelating agents or by treatment with SDS and 2-mercaptoethanol Escherichia coli
4.1.2.19 Mn2+ restores activity of the enzyme after inactivation by dialysis against chelating agents or by treatment with SDS and 2-mercaptoethanol Escherichia coli
4.1.2.19 Na+ Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity Escherichia coli
4.1.2.19 NH4+ Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity Escherichia coli
4.1.2.19 Ni2+ restores activity of the enzyme after inactivation by dialysis against chelating agents or by treatment with SDS and 2-mercaptoethanol Escherichia coli
4.1.2.19 Rb+ Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity Escherichia coli
4.1.2.19 Zn zinc metalloenzyme Escherichia coli
4.1.2.19 Zn contains 2 gatom of zinc per mol of enzyme Escherichia coli
4.1.2.19 Zn2+ restores activity of the enzyme after inactivation by dialysis against chelating agents or by treatment with SDS and 2-mercaptoethanol Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.19 35000
-
 4 * 35000 Escherichia coli
4.1.2.19 130000 140000 sucrose density gradient centrifugation Escherichia coli
4.1.2.19 135000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.17 Escherichia coli
-
-
-
4.1.2.17 Escherichia coli 0111-B4
-
-
-
4.1.2.19 Escherichia coli
-
-
-
4.1.2.19 Lactiplantibacillus plantarum
-
-
-

Storage Stability

EC Number Storage Stability Organism
4.1.2.19 0-4°C, pH 7.0, 0.01 M mercaptoethanol, stable for at least 3 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.17 Glycerone phosphate + (R)-lactaldehyde at 73% of the activity with (S)-lactaldehyde Escherichia coli 6-Deoxy-D-psicose 1-phosphate
-
 ?
4.1.2.17 Glycerone phosphate + (R)-lactaldehyde at 73% of the activity with (S)-lactaldehyde Escherichia coli 0111-B4 6-Deoxy-D-psicose 1-phosphate
-
 ?
4.1.2.17 glycerone phosphate + D-glyceraldehyde at 33% of the activity with (S)-lactaldehyde Escherichia coli D-psicose 1-phosphate
-
 ?
4.1.2.17 glycerone phosphate + D-glyceraldehyde at 33% of the activity with (S)-lactaldehyde Escherichia coli 0111-B4 D-psicose 1-phosphate
-
 ?
4.1.2.17 Glycerone phosphate + glycolaldehyde at 10% of the activity with (S)-lactaldehyde Escherichia coli D-Ribulose 1-phosphate
-
 ?
4.1.2.17 Glycerone phosphate + glycolaldehyde at 10% of the activity with (S)-lactaldehyde Escherichia coli 0111-B4 D-Ribulose 1-phosphate
-
 ?
4.1.2.17 glycerone phosphate + L-glyceraldehyde at 33% of the activity with (S)-lactaldehyde Escherichia coli L-tagatose 1-phosphate
-
 ?
4.1.2.17 glycerone phosphate + L-glyceraldehyde at 33% of the activity with (S)-lactaldehyde Escherichia coli 0111-B4 L-tagatose 1-phosphate
-
 ?
4.1.2.17 L-fuculose 1-phosphate
-
 Escherichia coli glycerone phosphate + (S)-lactaldehyde
-
 ?
4.1.2.17 L-fuculose 1-phosphate
-
 Escherichia coli 0111-B4 glycerone phosphate + (S)-lactaldehyde
-
 ?
4.1.2.19 Glycerone phosphate + (S)-lactaldehyde
-
 Escherichia coli L-Rhamnulose 1-phosphate
-
 ?
4.1.2.19 Glycerone phosphate + acetaldehyde
-
 Escherichia coli 5-Deoxy-L-xylulose 1-phosphate
-
 ?
4.1.2.19 glycerone phosphate + D-glyceraldehyde
-
 Escherichia coli D-sorbose 1-phosphate
-
 ?
4.1.2.19 Glycerone phosphate + D-lactaldehyde
-
 Escherichia coli 6-Deoxy-D-sorbose 1-phosphate
-
 ?
4.1.2.19 Glycerone phosphate + formaldehyde
-
 Escherichia coli D-Erythrulose 1-phosphate
-
 ?
4.1.2.19 Glycerone phosphate + glycolaldehyde
-
 Escherichia coli L-Xylulose 1-phosphate
-
 ?
4.1.2.19 L-Rhamnulose 1-phosphate
-
 Lactiplantibacillus plantarum Glycerone phosphate + (S)-lactaldehyde
-
 ?
4.1.2.19 L-Rhamnulose 1-phosphate
-
 Escherichia coli Glycerone phosphate + (S)-lactaldehyde
-
 ?
4.1.2.19 L-Sorbose 1-phosphate
-
 Escherichia coli Glycerone phosphate + D-glyceraldehyde
-
 ?
4.1.2.19 Xylulose 1-phosphate
-
 Escherichia coli Glycerone phosphate + glycoaldehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.19 tetramer 4 * 35000 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.19 38.3
-
 L-rhamnulose 1-phosphate
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.17 7.2
-
-
 Escherichia coli
4.1.2.19 7.5
-
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.19 7
-
 0-4°C, stable for at least 3 months Escherichia coli