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Literature summary extracted from

  • Stoffel, W.; Lekim, D; Stich, G.
    Distribution and properties of dihydrosphingosine-1-phosphate aldolase (Sphinganine-1-phosphate alkanal-lyase) (1969), Hoppe-Seyler's Z. Physiol. Chem., 350, 1233-1241.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.2.27 stable towards Triton X-100 and no significant inhibition of the enzyme is observed up to a concentration of 15 mg/ml Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.27 Deoxypyridoxine phosphate 50% inhibition at a concentration of 1.5 mM Rattus norvegicus
4.1.2.27 iodoacetamide complete inhibition Rattus norvegicus
4.1.2.27 N-ethylmaleimide complete inhibition Rattus norvegicus
4.1.2.27 p-chloromercuribenzoate complete inhibition Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.2.27 endoplasmic reticulum membrane-bound enzyme Rattus norvegicus 5783
-
4.1.2.27 endoplasmic reticulum membrane-bound enzyme Sus scrofa 5783
-
4.1.2.27 endoplasmic reticulum membrane-bound enzyme Bos taurus 5783
-
4.1.2.27 endoplasmic reticulum membrane-bound enzyme Wickerhamomyces ciferrii 5783
-
4.1.2.27 microsome
-
 Sus scrofa
-
-
4.1.2.27 microsome
-
 Bos taurus
-
-
4.1.2.27 microsome highest enzyme activity Rattus norvegicus
-
-
4.1.2.27 microsome highest enzyme activity Wickerhamomyces ciferrii
-
-
4.1.2.27 mitochondrial inner membrane inner mitochondrial membrane possesses the highest activity Rattus norvegicus 5743
-
4.1.2.27 mitochondrial inner membrane membrane-bound Rattus norvegicus 5743
-
4.1.2.27 mitochondrial inner membrane membrane-bound Sus scrofa 5743
-
4.1.2.27 mitochondrial inner membrane membrane-bound Bos taurus 5743
-
4.1.2.27 mitochondrial inner membrane membrane-bound Wickerhamomyces ciferrii 5743
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.27 F- NaF required to inhibit phosphatases which rapidly hydrolyse the substrate sphinganine 1-phosphate and the coenzyme pyridoxal phosphate Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.27 additional information Sus scrofa
-
 ?
-
 ?
4.1.2.27 additional information Bos taurus
-
 ?
-
 ?
4.1.2.27 additional information Wickerhamomyces ciferrii
-
 ?
-
 ?
4.1.2.27 additional information Rattus norvegicus metabolic relationship between substrates and products of biosynthesis and degradation of sphingosine bases ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.27 Bos taurus
-
-
-
4.1.2.27 Rattus norvegicus
-
-
-
4.1.2.27 Sus scrofa
-
-
-
4.1.2.27 Wickerhamomyces ciferrii
-
 yeast
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.2.27 Sphinganine 1-phosphate = phosphoethanolamine + palmitaldehyde irreversible reaction Rattus norvegicus
a
4.1.2.27 Sphinganine 1-phosphate = phosphoethanolamine + palmitaldehyde pyridoxal phosphate dependent catalysis Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.27 brain
-
 Rattus norvegicus
-
4.1.2.27 heart
-
 Rattus norvegicus
-
4.1.2.27 kidney
-
 Rattus norvegicus
-
4.1.2.27 kidney
-
 Sus scrofa
-
4.1.2.27 liver
-
 Rattus norvegicus
-
4.1.2.27 liver
-
 Sus scrofa
-
4.1.2.27 liver
-
 Bos taurus
-
4.1.2.27 lung
-
 Rattus norvegicus
-
4.1.2.27 muscle
-
 Rattus norvegicus
-
4.1.2.27 spleen
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.27 4D-Hydroxysphinganine 1-phosphate
-
 Rattus norvegicus Phosphoethanolamine + 2-hydroxyhexadecanal
-
 ?
4.1.2.27 4D-Hydroxysphinganine 1-phosphate
-
 Wickerhamomyces ciferrii Phosphoethanolamine + 2-hydroxyhexadecanal
-
 ?
4.1.2.27 additional information
-
 Rattus norvegicus ?
-
 ?
4.1.2.27 additional information
-
 Sus scrofa ?
-
 ?
4.1.2.27 additional information
-
 Bos taurus ?
-
 ?
4.1.2.27 additional information
-
 Wickerhamomyces ciferrii ?
-
 ?
4.1.2.27 additional information metabolic relationship between substrates and products of biosynthesis and degradation of sphingosine bases Rattus norvegicus ?
-
 ?
4.1.2.27 sphinganine 1-phosphate dihydrosphingosine-1-phosphate Rattus norvegicus phosphoethanolamine + palmitaldehyde
-
 ?
4.1.2.27 sphinganine 1-phosphate dihydrosphingosine-1-phosphate Sus scrofa phosphoethanolamine + palmitaldehyde
-
 ?
4.1.2.27 sphinganine 1-phosphate dihydrosphingosine-1-phosphate Bos taurus phosphoethanolamine + palmitaldehyde
-
 ?
4.1.2.27 sphinganine 1-phosphate dihydrosphingosine-1-phosphate Wickerhamomyces ciferrii phosphoethanolamine + palmitaldehyde
-
 ?
4.1.2.27 sphinganine 1-phosphate erythro-DL-dihydrosphingosine 1-phosphate Rattus norvegicus phosphoethanolamine + palmitaldehyde
-
 ?
4.1.2.27 sphinganine 1-phosphate threo-dihydrosphingosine 1-phosphate Rattus norvegicus phosphoethanolamine + palmitaldehyde
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.27 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.27 7.4 7.6
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.27 pyridoxal 5'-phosphate
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.2.27 1.5
-
 Deoxypyridoxine phosphate
-
 Rattus norvegicus