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Literature summary extracted from

  • Weber, T.; Bach, T.J.
    Partial purification and characterization of membrane-associated 3-hydroxy-3-methylglutaryl coenzyme A lyase from radish seedlings (1993), Z. Naturforsch. C, 48, 444-450.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.1.3.4 extremely unstable Raphanus sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.4 0.04
-
 hydroxymethylglutaryl-CoA
-
 Raphanus sativus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.3.4 membrane
-
 Raphanus sativus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.4 Bivalent cations
-
 Raphanus sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.4 70000
-
 gel filtration Raphanus sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.4 (S)-3-Hydroxy-3-methylglutaryl-CoA Raphanus sativus involved in regulation of isoprenoid pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.4 Raphanus sativus
-
 radish
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.4
-
 Raphanus sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.4 seedling
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.4 0.0098
-
-
 Raphanus sativus

Storage Stability

EC Number Storage Stability Organism
4.1.3.4 -80°C, several weeks Raphanus sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.4 (S)-3-Hydroxy-3-methylglutaryl-CoA involved in regulation of isoprenoid pathway Raphanus sativus ?
-
 ?
4.1.3.4 3-hydroxy-3-methylglutaryl-CoA
-
 Raphanus sativus acetoacetate + acetyl-CoA
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.4 30
-
 rapidly inactivated above Raphanus sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.4 8
-
-
 Raphanus sativus