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Literature summary extracted from

  • Toraya, T.; Fukui, S.
    Diol dehydrase (1982), B12 (Dolphin, D. ed. ) Wiley, New York, 2, 233-262.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.2.1.28 10-20% glycerol stabilizes component F at 0°C Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.28 adenosylcobalamin Ki: 0.8 mM Klebsiella pneumoniae
4.2.1.28 Chloroacetaldehyde inactivates Klebsiella pneumoniae
4.2.1.28 EDTA
-
 Citrobacter freundii
4.2.1.28 EDTA
-
 Klebsiella pneumoniae
4.2.1.28 EDTA
-
 Propionibacterium freudenreichii
4.2.1.28 glycerol stereospecificity: enzyme-R-glycerol complex responsible for product-formation, enzyme-S-glycerol complex responsible for inactivation, several molecules of glycerol per enzyme molecule involved, active-site inhibition, C3-methyl group plays crucial role Klebsiella pneumoniae
4.2.1.28 glycoaldehyde inactivates Klebsiella pneumoniae
4.2.1.28 p-chloromercuribenzoate reversed by thiols Klebsiella pneumoniae
4.2.1.28 p-chloromercuribenzoate
-
 Propionibacterium freudenreichii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.28 additional information
-
 additional information
-
 Klebsiella pneumoniae
4.2.1.28 additional information
-
 additional information
-
 Citrobacter freundii
4.2.1.28 additional information
-
 additional information
-
 Propionibacterium freudenreichii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.28 Cs+
-
 Propionibacterium freudenreichii
4.2.1.28 Cs+ activates, Km: 5.0 mM Klebsiella pneumoniae
4.2.1.28 K+ requirement for monovalent cation Klebsiella pneumoniae
4.2.1.28 K+ requirement for monovalent cation Citrobacter freundii
4.2.1.28 K+ requirement for monovalent cation Propionibacterium freudenreichii
4.2.1.28 K+ Km: 0.67 mM Klebsiella pneumoniae
4.2.1.28 Na+
-
 Propionibacterium freudenreichii
4.2.1.28 Na+ activates, Km: 19 mM Klebsiella pneumoniae
4.2.1.28 NH4+
-
 Propionibacterium freudenreichii
4.2.1.28 NH4+ Km: 0.12 mM Klebsiella pneumoniae
4.2.1.28 Rb+
-
 Propionibacterium freudenreichii
4.2.1.28 Rb+ Km: 1.8 mM Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.28 additional information
-
-
 Klebsiella pneumoniae
4.2.1.28 additional information
-
-
 Citrobacter freundii
4.2.1.28 additional information
-
-
 Propionibacterium freudenreichii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.28 1,2-propanediol Klebsiella pneumoniae
-
 ?
-
 ?
4.2.1.28 glycerol Klebsiella pneumoniae first step of anaerobic fermentation ?
-
 ?
4.2.1.28 glycerol Klebsiella pneumoniae different enzyme than glycerol dehydrase ?
-
 ?
4.2.1.28 additional information Klebsiella pneumoniae metabolic role: production of electron acceptor beta-hydroxypropionaldehyde ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.28 Citrobacter freundii
-
-
-
4.2.1.28 Klebsiella pneumoniae
-
 formerly Aerobacter aerogenes
-
4.2.1.28 Propionibacterium freudenreichii
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.1.28 total inactivation after aerobic incubation, substrate and analogues protect Klebsiella pneumoniae

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.28 propane-1,2-diol = propanal + H2O mechanism Klebsiella pneumoniae
a
4.2.1.28 propane-1,2-diol = propanal + H2O general mechanism and stereochemistry, role of coenzyme in OH-group transfer, mechanism of overall reaction, role of free radicals during reaction, studies with coenzyme analogues Klebsiella pneumoniae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.28 1,2-propanediol
-
 Klebsiella pneumoniae propanal
-
 ?
4.2.1.28 1,2-propanediol
-
 Citrobacter freundii propanal
-
 ?
4.2.1.28 1,2-propanediol
-
 Propionibacterium freudenreichii propanal
-
 ?
4.2.1.28 1,2-propanediol
-
 Klebsiella pneumoniae ?
-
 ?
4.2.1.28 glycerol first step of anaerobic fermentation Klebsiella pneumoniae ?
-
 ?
4.2.1.28 glycerol different enzyme than glycerol dehydrase Klebsiella pneumoniae ?
-
 ?
4.2.1.28 additional information
-
 Klebsiella pneumoniae ?
-
 ?
4.2.1.28 additional information metabolic role: production of electron acceptor beta-hydroxypropionaldehyde Klebsiella pneumoniae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.28 More
-
 Citrobacter freundii
4.2.1.28 More
-
 Propionibacterium freudenreichii
4.2.1.28 More reassociatin of components F and S strongly promoted by substrate, component S is a sulfhydryl protein Klebsiella pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.28 additional information
-
 additional information
-
 Klebsiella pneumoniae
4.2.1.28 additional information
-
 additional information
-
 Citrobacter freundii
4.2.1.28 additional information
-
 additional information
-
 Propionibacterium freudenreichii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.28 6.5 9.5
-
 Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.28 cobamide
-
 Citrobacter freundii
4.2.1.28 cobamide
-
 Propionibacterium freudenreichii
4.2.1.28 cobamide slow inactivation by analogues Klebsiella pneumoniae
4.2.1.28 cobamide coenzyme serves as intermediate hydrogen carrier Klebsiella pneumoniae
4.2.1.28 cobamide mechanism of coenzyme action Klebsiella pneumoniae