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Literature summary extracted from
- The purification and properties of urocanase from Pseudomonas testosteroni (1978), Biochem. J., 171, 41-50.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.49 | Cu2+ | non-competitive | Comamonas testosteroni |  |
| 4.2.1.49 | Hg2+ | - |
Comamonas testosteroni | |
| 4.2.1.49 | hydroxylamine | - |
Bos taurus | |
| 4.2.1.49 | hydroxylamine | 10 mM, complete inhibition | Comamonas testosteroni | |
| 4.2.1.49 | additional information | - |
Bos taurus | |
| 4.2.1.49 | additional information | overview | Comamonas testosteroni | |
| 4.2.1.49 | NaBH4 | - |
Bos taurus | |
| 4.2.1.49 | NaBH4 | strong | Comamonas testosteroni | |
| 4.2.1.49 | phenylhydrazine | - |
Comamonas testosteroni | |
| 4.2.1.49 | Semicarbazide | - |
Bos taurus | |
| 4.2.1.49 | Semicarbazide | - |
Comamonas testosteroni | |
| 4.2.1.49 | sulfite | - |
Bos taurus | |
| 4.2.1.49 | sulfite | NaHSO3 | Comamonas testosteroni | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.49 | 0.248 | - |
Urocanate | - |
Comamonas testosteroni | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.49 | 58000 | - |
2 * 58000, SDS-PAGE | Comamonas testosteroni |
| 4.2.1.49 | 118000 | - |
meniscus depletion method | Comamonas testosteroni |
| 4.2.1.49 | 120000 | - |
sucrose density gradient centrifugation | Bacillus subtilis |
| 4.2.1.49 | 127000 | - |
gel filtration | Felis catus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.49 | Urocanate + H2O | Gallus gallus | - |
? | - |
? | |
| 4.2.1.49 | Urocanate + H2O | Bacillus subtilis | - |
? | - |
? | |
| 4.2.1.49 | Urocanate + H2O | Bos taurus | - |
? | - |
? | |
| 4.2.1.49 | Urocanate + H2O | Klebsiella aerogenes | - |
? | - |
? | |
| 4.2.1.49 | Urocanate + H2O | Felis catus | - |
? | - |
? | |
| 4.2.1.49 | Urocanate + H2O | Comamonas testosteroni | - |
? | - |
? | |
| 4.2.1.49 | Urocanate + H2O | Comamonas testosteroni N.C.I.B. 10808 | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.49 | Bacillus subtilis | - |
- |
- |
| 4.2.1.49 | Bos taurus | - |
- |
- |
| 4.2.1.49 | Comamonas testosteroni | - |
N.C.I.B. 10808 | - |
| 4.2.1.49 | Comamonas testosteroni N.C.I.B. 10808 | - |
N.C.I.B. 10808 | - |
| 4.2.1.49 | Felis catus | - |
- |
- |
| 4.2.1.49 | Gallus gallus | - |
- |
- |
| 4.2.1.49 | Klebsiella aerogenes | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.49 | - |
Comamonas testosteroni |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.1.49 | liver | - |
Gallus gallus | - |
| 4.2.1.49 | liver | - |
Bos taurus | - |
| 4.2.1.49 | liver | - |
Felis catus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.49 | 0.248 | - |
- |
Comamonas testosteroni |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.2.1.49 | -16¦C, lyophilized, stable for several weeks | Bos taurus |
| 4.2.1.49 | -18¦C, 0.1 M phosphate buffer, pH 7.0, stable for many weeks | Comamonas testosteroni |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.49 | Urocanate + H2O | - |
Gallus gallus | 4,5-Dihydro-4-oxo-5-imidazolepropanoate | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Bacillus subtilis | 4,5-Dihydro-4-oxo-5-imidazolepropanoate | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Bos taurus | 4,5-Dihydro-4-oxo-5-imidazolepropanoate | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Klebsiella aerogenes | 4,5-Dihydro-4-oxo-5-imidazolepropanoate | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Felis catus | 4,5-Dihydro-4-oxo-5-imidazolepropanoate | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Comamonas testosteroni | 4,5-Dihydro-4-oxo-5-imidazolepropanoate | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Comamonas testosteroni N.C.I.B. 10808 | 4,5-Dihydro-4-oxo-5-imidazolepropanoate | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Gallus gallus | ? | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Bacillus subtilis | ? | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Bos taurus | ? | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Klebsiella aerogenes | ? | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Felis catus | ? | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Comamonas testosteroni | ? | - |
? | |
| 4.2.1.49 | Urocanate + H2O | - |
Comamonas testosteroni N.C.I.B. 10808 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.49 | dimer | - |
Bacillus subtilis |
| 4.2.1.49 | dimer | 2 * 58000, SDS-PAGE | Comamonas testosteroni |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.49 | 7.2 | - |
- |
Bos taurus |
| 4.2.1.49 | 7.2 | - |
- |
Comamonas testosteroni |
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