EC Number | General Stability | Organism |
---|---|---|
2.5.1.51 | freeze-thawing or lyophilization inactivates | Citrullus lanatus subsp. vulgaris |
2.5.1.51 | sensitive to freezing even in the presence of 20% glycerol | Citrullus lanatus subsp. vulgaris |
4.2.1.50 | sensitive to freezing even in 20% glycerol | Citrullus lanatus subsp. vulgaris |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.51 | hydroxylamine | 50 mM, almost complete inhibition | Citrullus lanatus subsp. vulgaris | |
2.5.1.51 | KCN | 10 mM, almost complete inhibition | Citrullus lanatus subsp. vulgaris | |
4.2.1.50 | hydroxylamine | 50 mM almost complete inhibition | Citrullus lanatus subsp. vulgaris | |
4.2.1.50 | potassium cyanide | 10 mM almost complete inhibition | Citrullus lanatus subsp. vulgaris |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.51 | 2.5 | - |
O-acetylserine | - |
Citrullus lanatus subsp. vulgaris | |
2.5.1.51 | 74 | - |
pyrazole | - |
Citrullus lanatus subsp. vulgaris | |
4.2.1.50 | 2.5 | - |
O-acetyl-L-serine | - |
Citrullus lanatus subsp. vulgaris | |
4.2.1.50 | 74 | - |
pyrazole | - |
Citrullus lanatus subsp. vulgaris |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.51 | 32000 | - |
2 * 32000, SDS-PAGE | Citrullus lanatus subsp. vulgaris |
2.5.1.51 | 58000 | - |
gel filtration | Citrullus lanatus subsp. vulgaris |
4.2.1.50 | 32000 | - |
2 * 32000, SDS-PAGE | Citrullus lanatus subsp. vulgaris |
4.2.1.50 | 58000 | - |
gel filtration | Citrullus lanatus subsp. vulgaris |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.51 | O3-acetyl-L-serine + pyrazole | Citrullus lanatus subsp. vulgaris | involved in biosynthesis of heterocyclic beta-substituted alanines | 3-(pyrazol-1-yl)-L-alanine + acetate | - |
? | |
4.2.1.50 | O-Acetyl-L-serine + pyrazole | Citrullus lanatus subsp. vulgaris | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.51 | Citrullus lanatus subsp. vulgaris | - |
- |
- |
4.2.1.50 | Citrullus lanatus subsp. vulgaris | - |
water melon | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.51 | partial | Citrullus lanatus subsp. vulgaris |
4.2.1.50 | - |
Citrullus lanatus subsp. vulgaris |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.51 | additional information | barely detectable in dry seeds | Citrullus lanatus subsp. vulgaris | - |
2.5.1.51 | seedling | cotyledons removed, 1 week old | Citrullus lanatus subsp. vulgaris | - |
4.2.1.50 | seedling | - |
Citrullus lanatus subsp. vulgaris | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.51 | 0.355 | - |
- |
Citrullus lanatus subsp. vulgaris |
4.2.1.50 | 0.355 | - |
- |
Citrullus lanatus subsp. vulgaris |
EC Number | Storage Stability | Organism |
---|---|---|
2.5.1.51 | 0-2°C, stable for at least 1 month | Citrullus lanatus subsp. vulgaris |
4.2.1.50 | 0¦C, 1 month | Citrullus lanatus subsp. vulgaris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.51 | O-succinyl-L-serine + pyrazole | at 16% the rate of O-acetyl-L-serine | Citrullus lanatus subsp. vulgaris | 3-(pyrazol-1-yl)-L-alanine + succinate | - |
? | |
2.5.1.51 | O-sulfo-L-serine + pyrazole | at 2.5% the rate of O-acetyl-L-serine | Citrullus lanatus subsp. vulgaris | 3-(pyrazol-1-yl)-L-alanine + SO42- | - |
? | |
2.5.1.51 | O3-acetyl-L-serine + 3-amino-1,2,4-triazole | at 11% the rate of pyrazole | Citrullus lanatus subsp. vulgaris | 3-(3-amino-1,2,4-triazol-1-yl)-L-alanine + acetate | - |
? | |
2.5.1.51 | O3-acetyl-L-serine + N-hydroxyurea | at 9% the rate of pyrazole | Citrullus lanatus subsp. vulgaris | O-ureidoserine + acetate | - |
? | |
2.5.1.51 | O3-acetyl-L-serine + pyrazole | highly specific | Citrullus lanatus subsp. vulgaris | 3-(pyrazol-1-yl)-L-alanine + acetate | - |
? | |
2.5.1.51 | O3-acetyl-L-serine + pyrazole | involved in biosynthesis of heterocyclic beta-substituted alanines | Citrullus lanatus subsp. vulgaris | 3-(pyrazol-1-yl)-L-alanine + acetate | - |
? | |
4.2.1.50 | additional information | no activity with L-serine | Citrullus lanatus subsp. vulgaris | ? | - |
? | |
4.2.1.50 | O-Acetyl-L-serine + 3-amino-1,2,4-triazole | 10.6% of activity with pyrazole | Citrullus lanatus subsp. vulgaris | ? | - |
? | |
4.2.1.50 | O-Acetyl-L-serine + N-hydroxyurea | 8.7% of activity with pyrazole | Citrullus lanatus subsp. vulgaris | ? | - |
? | |
4.2.1.50 | O-Acetyl-L-serine + pyrazole | - |
Citrullus lanatus subsp. vulgaris | 3-(Pyrazol-1-yl)-L-alanine + acetate | - |
? | |
4.2.1.50 | O-Acetyl-L-serine + pyrazole | - |
Citrullus lanatus subsp. vulgaris | ? | - |
? | |
4.2.1.50 | O-Succinyl-L-serine + pyrazole | 16.4% of activity with O-Acetyl-L-serine | Citrullus lanatus subsp. vulgaris | ? | - |
? | |
4.2.1.50 | O-Sulfo-L-serine + pyrazole | 2.5% of activity with O-Acetyl-L-serine | Citrullus lanatus subsp. vulgaris | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.51 | dimer | 2 * 32000, SDS-PAGE | Citrullus lanatus subsp. vulgaris |
4.2.1.50 | dimer | 2 * 32000, SDS-PAGE | Citrullus lanatus subsp. vulgaris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.51 | 7.3 | 7.4 | - |
Citrullus lanatus subsp. vulgaris |
4.2.1.50 | 7.3 | 7.4 | O-acetyl-L-serine + pyrazole | Citrullus lanatus subsp. vulgaris |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.51 | 6.5 | 8.5 | about half-maximal activity at pH 6.5 and 8.5 | Citrullus lanatus subsp. vulgaris |
4.2.1.50 | 6.5 | 8.5 | 50% of maximal activity with O-acetyl-L-serine + pyrazole at pH 6.5 and pH 8.5 | Citrullus lanatus subsp. vulgaris |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.51 | pyridoxal 5'-phosphate | requirement, enzyme bound, 1 mol pyridoxal 5'-phosphate per mol subunit, further increase of activity by 40% by adding pyridoxal phosphate | Citrullus lanatus subsp. vulgaris | |
4.2.1.50 | pyridoxal 5'-phosphate | 1 molecule bound to each subunit | Citrullus lanatus subsp. vulgaris | |
4.2.1.50 | pyridoxal 5'-phosphate | maximal activation by 40% at 0.25 mM | Citrullus lanatus subsp. vulgaris |