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Literature summary extracted from

  • Murakoshi, I.; Ikegami, F.; Hinuma, Y.; Hanma, Y.
    Purification and characterization of beta-(pyrazol-1-yl)-L-alanine synthase from Citrullus vulgaris (1984), Phytochemistry, 23, 973-977.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.5.1.51 freeze-thawing or lyophilization inactivates Citrullus lanatus subsp. vulgaris
2.5.1.51 sensitive to freezing even in the presence of 20% glycerol Citrullus lanatus subsp. vulgaris
4.2.1.50 sensitive to freezing even in 20% glycerol Citrullus lanatus subsp. vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.51 hydroxylamine 50 mM, almost complete inhibition Citrullus lanatus subsp. vulgaris
2.5.1.51 KCN 10 mM, almost complete inhibition Citrullus lanatus subsp. vulgaris
4.2.1.50 hydroxylamine 50 mM almost complete inhibition Citrullus lanatus subsp. vulgaris
4.2.1.50 potassium cyanide 10 mM almost complete inhibition Citrullus lanatus subsp. vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.51 2.5
-
O-acetylserine
-
Citrullus lanatus subsp. vulgaris
2.5.1.51 74
-
pyrazole
-
Citrullus lanatus subsp. vulgaris
4.2.1.50 2.5
-
O-acetyl-L-serine
-
Citrullus lanatus subsp. vulgaris
4.2.1.50 74
-
pyrazole
-
Citrullus lanatus subsp. vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.51 32000
-
2 * 32000, SDS-PAGE Citrullus lanatus subsp. vulgaris
2.5.1.51 58000
-
gel filtration Citrullus lanatus subsp. vulgaris
4.2.1.50 32000
-
2 * 32000, SDS-PAGE Citrullus lanatus subsp. vulgaris
4.2.1.50 58000
-
gel filtration Citrullus lanatus subsp. vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.51 O3-acetyl-L-serine + pyrazole Citrullus lanatus subsp. vulgaris involved in biosynthesis of heterocyclic beta-substituted alanines 3-(pyrazol-1-yl)-L-alanine + acetate
-
?
4.2.1.50 O-Acetyl-L-serine + pyrazole Citrullus lanatus subsp. vulgaris
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.51 Citrullus lanatus subsp. vulgaris
-
-
-
4.2.1.50 Citrullus lanatus subsp. vulgaris
-
water melon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.51 partial Citrullus lanatus subsp. vulgaris
4.2.1.50
-
Citrullus lanatus subsp. vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.51 additional information barely detectable in dry seeds Citrullus lanatus subsp. vulgaris
-
2.5.1.51 seedling cotyledons removed, 1 week old Citrullus lanatus subsp. vulgaris
-
4.2.1.50 seedling
-
Citrullus lanatus subsp. vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.51 0.355
-
-
Citrullus lanatus subsp. vulgaris
4.2.1.50 0.355
-
-
Citrullus lanatus subsp. vulgaris

Storage Stability

EC Number Storage Stability Organism
2.5.1.51 0-2°C, stable for at least 1 month Citrullus lanatus subsp. vulgaris
4.2.1.50 0¦C, 1 month Citrullus lanatus subsp. vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.51 O-succinyl-L-serine + pyrazole at 16% the rate of O-acetyl-L-serine Citrullus lanatus subsp. vulgaris 3-(pyrazol-1-yl)-L-alanine + succinate
-
?
2.5.1.51 O-sulfo-L-serine + pyrazole at 2.5% the rate of O-acetyl-L-serine Citrullus lanatus subsp. vulgaris 3-(pyrazol-1-yl)-L-alanine + SO42-
-
?
2.5.1.51 O3-acetyl-L-serine + 3-amino-1,2,4-triazole at 11% the rate of pyrazole Citrullus lanatus subsp. vulgaris 3-(3-amino-1,2,4-triazol-1-yl)-L-alanine + acetate
-
?
2.5.1.51 O3-acetyl-L-serine + N-hydroxyurea at 9% the rate of pyrazole Citrullus lanatus subsp. vulgaris O-ureidoserine + acetate
-
?
2.5.1.51 O3-acetyl-L-serine + pyrazole highly specific Citrullus lanatus subsp. vulgaris 3-(pyrazol-1-yl)-L-alanine + acetate
-
?
2.5.1.51 O3-acetyl-L-serine + pyrazole involved in biosynthesis of heterocyclic beta-substituted alanines Citrullus lanatus subsp. vulgaris 3-(pyrazol-1-yl)-L-alanine + acetate
-
?
4.2.1.50 additional information no activity with L-serine Citrullus lanatus subsp. vulgaris ?
-
?
4.2.1.50 O-Acetyl-L-serine + 3-amino-1,2,4-triazole 10.6% of activity with pyrazole Citrullus lanatus subsp. vulgaris ?
-
?
4.2.1.50 O-Acetyl-L-serine + N-hydroxyurea 8.7% of activity with pyrazole Citrullus lanatus subsp. vulgaris ?
-
?
4.2.1.50 O-Acetyl-L-serine + pyrazole
-
Citrullus lanatus subsp. vulgaris 3-(Pyrazol-1-yl)-L-alanine + acetate
-
?
4.2.1.50 O-Acetyl-L-serine + pyrazole
-
Citrullus lanatus subsp. vulgaris ?
-
?
4.2.1.50 O-Succinyl-L-serine + pyrazole 16.4% of activity with O-Acetyl-L-serine Citrullus lanatus subsp. vulgaris ?
-
?
4.2.1.50 O-Sulfo-L-serine + pyrazole 2.5% of activity with O-Acetyl-L-serine Citrullus lanatus subsp. vulgaris ?
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.51 dimer 2 * 32000, SDS-PAGE Citrullus lanatus subsp. vulgaris
4.2.1.50 dimer 2 * 32000, SDS-PAGE Citrullus lanatus subsp. vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.51 7.3 7.4
-
Citrullus lanatus subsp. vulgaris
4.2.1.50 7.3 7.4 O-acetyl-L-serine + pyrazole Citrullus lanatus subsp. vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.51 6.5 8.5 about half-maximal activity at pH 6.5 and 8.5 Citrullus lanatus subsp. vulgaris
4.2.1.50 6.5 8.5 50% of maximal activity with O-acetyl-L-serine + pyrazole at pH 6.5 and pH 8.5 Citrullus lanatus subsp. vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.51 pyridoxal 5'-phosphate requirement, enzyme bound, 1 mol pyridoxal 5'-phosphate per mol subunit, further increase of activity by 40% by adding pyridoxal phosphate Citrullus lanatus subsp. vulgaris
4.2.1.50 pyridoxal 5'-phosphate 1 molecule bound to each subunit Citrullus lanatus subsp. vulgaris
4.2.1.50 pyridoxal 5'-phosphate maximal activation by 40% at 0.25 mM Citrullus lanatus subsp. vulgaris