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Literature summary extracted from

  • Burnell, J.N.; Whatley, F.R.
    Sulphur metabolism in Paracoccus denitrificans. Purification, properties and regulation of cysteinyl- and methionyl-tRNA synthetase (1977), Biochim. Biophys. Acta, 481, 266-278.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.10 cysteic acid stimulates Paracoccus denitrificans
6.1.1.10 cysteine stimulates Paracoccus denitrificans
6.1.1.10 SO32- stimulates Paracoccus denitrificans
6.1.1.16 methionine stimulates Paracoccus denitrificans

General Stability

EC Number General Stability Organism
6.1.1.10 stability is increased by presence of a reducing agent. In the presence of 5 mM DTT purified enzyme is stable for at least 8 months at -15°C Paracoccus denitrificans

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.10 3'-AMP
-
 Paracoccus denitrificans
6.1.1.10 5'-AMP
-
 Paracoccus denitrificans
6.1.1.10 ADP
-
 Paracoccus denitrificans
6.1.1.10 ATP in free form Paracoccus denitrificans
6.1.1.10 D-methionine
-
 Paracoccus denitrificans
6.1.1.10 EDTA 10 mM, weak Paracoccus denitrificans
6.1.1.10 glutathione
-
 Paracoccus denitrificans
6.1.1.10 iodoacetamide 10 mM, weak Paracoccus denitrificans
6.1.1.10 Mg2+ in free form Paracoccus denitrificans
6.1.1.10 NaF 10 mM, weak Paracoccus denitrificans
6.1.1.10 NEM
-
 Paracoccus denitrificans
6.1.1.10 O-acetylserine
-
 Paracoccus denitrificans
6.1.1.10 p-chloromercuribenzoate
-
 Paracoccus denitrificans
6.1.1.16 2-L-aminobutyric acid
-
 Paracoccus denitrificans
6.1.1.16 3'-AMP
-
 Paracoccus denitrificans
6.1.1.16 5'-AMP
-
 Paracoccus denitrificans
6.1.1.16 ADP
-
 Paracoccus denitrificans
6.1.1.16 ATP free ATP inhibits Paracoccus denitrificans
6.1.1.16 cysteamine
-
 Paracoccus denitrificans
6.1.1.16 D-cysteine
-
 Paracoccus denitrificans
6.1.1.16 EDTA
-
 Paracoccus denitrificans
6.1.1.16 glutathione
-
 Paracoccus denitrificans
6.1.1.16 iodoacetamide
-
 Paracoccus denitrificans
6.1.1.16 L-alanine
-
 Paracoccus denitrificans
6.1.1.16 L-cysteic acid
-
 Paracoccus denitrificans
6.1.1.16 L-homocysteine
-
 Paracoccus denitrificans
6.1.1.16 L-selenocysteine
-
 Paracoccus denitrificans
6.1.1.16 Mg2+ free Mg2+ inhibits Paracoccus denitrificans
6.1.1.16 NaF weak Paracoccus denitrificans
6.1.1.16 NEM
-
 Paracoccus denitrificans
6.1.1.16 O-acetylserine
-
 Paracoccus denitrificans
6.1.1.16 p-chloromercuribenzoate
-
 Paracoccus denitrificans
6.1.1.16 phenylhydrazine weak Paracoccus denitrificans
6.1.1.16 phosphate
-
 Paracoccus denitrificans
6.1.1.16 S2-
-
 Paracoccus denitrificans
6.1.1.16 Sulfhydryl group reagents
-
 Paracoccus denitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.10 0.26
-
 L-Met
-
 Paracoccus denitrificans
6.1.1.10 0.31
-
 selenomethionine
-
 Paracoccus denitrificans
6.1.1.10 0.383
-
 norleucine
-
 Paracoccus denitrificans
6.1.1.10 0.87
-
 ATP
-
 Paracoccus denitrificans
6.1.1.10 28.6
-
 norleucine
-
 Paracoccus denitrificans
6.1.1.16 0.00159
-
 selenocysteine ATP-diphosphate exchange Paracoccus denitrificans
6.1.1.16 0.0125
-
 L-Cys ATP-diphosphate exchange Paracoccus denitrificans
6.1.1.16 0.3
-
 diphosphate ATP-diphosphate exchange Paracoccus denitrificans
6.1.1.16 1
-
 diphosphate ATP-diphosphate exchange Paracoccus denitrificans
6.1.1.16 1.33
-
 ATP ATP-diphosphate exchange Paracoccus denitrificans
6.1.1.16 10
-
 2-aminobutyric acid ATP-diphosphate exchange Paracoccus denitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.10 Ca2+ supports methionine-dependent ATP-diphosphate exchange with 14% of the efficiency of Mg2+ Paracoccus denitrificans
6.1.1.10 Co2+ supports methionine-dependent ATP-diphosphate exchange with 20% of the efficiency of Mg2+ Paracoccus denitrificans
6.1.1.10 Mg2+ absolutely dependent on Paracoccus denitrificans
6.1.1.10 Mg2+ 2 Paracoccus denitrificans
6.1.1.10 Mn2+ supports methionine-dependent ATP-diphosphate exchange with 15% of the efficiency of Mg2+ Paracoccus denitrificans
6.1.1.16 Co2+ 13% of the efficiency of Mg2+ in activation of ATP-diphosphate exchange Paracoccus denitrificans
6.1.1.16 Mg2+ required for ATP-diphosphate exchange Paracoccus denitrificans
6.1.1.16 Mg2+ inhibited by free Mg2+ Paracoccus denitrificans
6.1.1.16 Mg2+ optimal Mg2+/ATP ratio is 2.5 Paracoccus denitrificans
6.1.1.16 Zn2+ 6% of the efficiency of Mg2+ in activation of ATP-diphosphate exchange Paracoccus denitrificans

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.10 Paracoccus denitrificans
-
-
-
6.1.1.10 Paracoccus denitrificans 8944
-
-
-
6.1.1.16 Paracoccus denitrificans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.10
-
 Paracoccus denitrificans
6.1.1.16
-
 Paracoccus denitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.16 additional information
-
-
 Paracoccus denitrificans

Storage Stability

EC Number Storage Stability Organism
6.1.1.10 -15°C, 5 mM DTT, purified enzyme stable for at least 8 months Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Paracoccus denitrificans AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Paracoccus denitrificans 8944 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-norleucine + tRNAMet
-
 Paracoccus denitrificans AMP + diphosphate + L-norleucyl-tRNAMet
-
 ?
6.1.1.10 ATP + methionine + hydroxylamine
-
 Paracoccus denitrificans methionine hydroxamate + AMP + diphosphate
-
 ?
6.1.1.10 ATP + methionine + hydroxylamine
-
 Paracoccus denitrificans 8944 methionine hydroxamate + AMP + diphosphate
-
 ?
6.1.1.10 ATP + selenomethionine + tRNAMet
-
 Paracoccus denitrificans AMP + diphosphate + selenomethionyl-tRNAMet
-
 ?
6.1.1.10 ATP + selenomethionine + tRNAMet
-
 Paracoccus denitrificans 8944 AMP + diphosphate + selenomethionyl-tRNAMet
-
 ?
6.1.1.10 additional information methionine-dependent ATP-diphosphate exchange Paracoccus denitrificans ?
-
 ?
6.1.1.10 additional information also catalyzes L-homocysteine, L-selenocysteine and norleucine-dependent ATP-diphosphate exchange Paracoccus denitrificans ?
-
 ?
6.1.1.10 additional information methionine-dependent ATP-diphosphate exchange Paracoccus denitrificans 8944 ?
-
 ?
6.1.1.10 additional information also catalyzes L-homocysteine, L-selenocysteine and norleucine-dependent ATP-diphosphate exchange Paracoccus denitrificans 8944 ?
-
 ?
6.1.1.16 ATP + 2-aminobutyric acid + tRNACys
-
 Paracoccus denitrificans AMP + diphosphate + L-alpha-aminobutyryl-tRNACys
-
 ?
6.1.1.16 ATP + L-cysteine + tRNACys
-
 Paracoccus denitrificans AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
6.1.1.16 ATP + selenocysteine + tRNACys
-
 Paracoccus denitrificans AMP + diphosphate + selenocysteinyl-tRNACys
-
 ?
6.1.1.16 additional information ATP-diphosphate exchange Paracoccus denitrificans ?
-
 ?
6.1.1.16 additional information selenocysteine can replace cysteine in ATP-diphosphate exchange Paracoccus denitrificans ?
-
 ?
6.1.1.16 additional information 2-aminobutyric acid can replace cysteine in ATP-diphosphate exchange Paracoccus denitrificans ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.10 8
-
-
 Paracoccus denitrificans
6.1.1.10 8 8.5
-
 Paracoccus denitrificans
6.1.1.16 7.5 8.5
-
 Paracoccus denitrificans