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Literature summary extracted from
- On the biosynthesis of pseudouridine and of pseudouridylic acid in Agrobacterium tumefaciens (1966), Can. J. Biochem., 44, 259-272.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.2.1.70 | enzyme retains its activity during dialysis | Agrobacterium tumefaciens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.70 | 0.67 | - |
Uracil | - |
Agrobacterium tumefaciens |  |
| 4.2.1.70 | 0.84 | - |
ribose 5-phosphate | - |
Agrobacterium tumefaciens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.70 | uracil + D-ribose 5-phosphate | Agrobacterium tumefaciens | biosynthetic pathway for pseudouridylic acid and pseudouridine | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.70 | Agrobacterium tumefaciens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.70 | - |
Agrobacterium tumefaciens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.70 | additional information | - |
- |
Agrobacterium tumefaciens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.70 | uracil + D-ribose 5-phosphate | in presence of sodium arsenate | Agrobacterium tumefaciens | pseudouridine 5'-phosphate + H2O | in presence of sodium arsenate | ? | |
| 4.2.1.70 | uracil + D-ribose 5-phosphate | biosynthetic pathway for pseudouridylic acid and pseudouridine | Agrobacterium tumefaciens | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.70 | 9 | - |
- |
Agrobacterium tumefaciens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.70 | 7 | 9.5 | pH 7.0 about 50% of maximal activity, pH 9.5 about 80% of maximal activity | Agrobacterium tumefaciens |
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Release 2023.1 (January 2023)
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