EC Number | General Stability | Organism |
---|---|---|
4.2.1.70 | mercaptoethanol is very effective in restoring the original activity of aged enzyme preparations | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.70 | iodoacetamide | 150 mM, in about 5 min all activity is lost | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.2.1.70 | tRNA | wild-type RNA, 50% inhibition at a concentration of 0.005 mM | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.70 | 50000 | - |
2 * 50000, glycerol gradient sedimentation, tRNA induced dimerization | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.70 | additional information | Salmonella enterica subsp. enterica serovar Typhimurium | pseudouridylate synthetase I is responsible for the biosynthesis of pseudouridine in the anticodon region of several tRNA species | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.70 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
TA265 (wild-type strain of LT-2) | - |
4.2.1.70 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
TA253 (carrying the hisT 1504 mutation) | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.70 | pseudouridylate synthetase I | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.70 | additional information | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.1.70 | 4°C, storage for 40 days, preincubation with 15 mM mercaptoethanol restores the original activity | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.70 | additional information | pseudouridylate synthetase I is responsible for the biosynthesis of pseudouridine in the anticodon region of several tRNA species | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
4.2.1.70 | uracil + D-ribose 5-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | pseudouridine 5'-phosphate + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.70 | dimer | 2 * 50000, glycerol gradient sedimentation, tRNA induced dimerization | Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.70 | monomer | 1* 50000 glycerol gradient sedimentation | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.70 | 0.005 | - |
tRNA | wild-type RNA | Salmonella enterica subsp. enterica serovar Typhimurium |