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Literature summary extracted from

  • Arena, F.; Ciliberto, G.; Ciampi, S.; Cortese, R.
    Purification of pseudouridylate synthetase I from Salmonella typhimurium (1978), Nucleic Acids Res., 5, 4523-4536.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
4.2.1.70 mercaptoethanol is very effective in restoring the original activity of aged enzyme preparations Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.70 iodoacetamide 150 mM, in about 5 min all activity is lost Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.70 tRNA wild-type RNA, 50% inhibition at a concentration of 0.005 mM Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.70 50000
-
2 * 50000, glycerol gradient sedimentation, tRNA induced dimerization Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.70 additional information Salmonella enterica subsp. enterica serovar Typhimurium pseudouridylate synthetase I is responsible for the biosynthesis of pseudouridine in the anticodon region of several tRNA species ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.70 Salmonella enterica subsp. enterica serovar Typhimurium
-
TA265 (wild-type strain of LT-2)
-
4.2.1.70 Salmonella enterica subsp. enterica serovar Typhimurium
-
TA253 (carrying the hisT 1504 mutation)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.70 pseudouridylate synthetase I Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.70 additional information
-
-
Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
4.2.1.70 4°C, storage for 40 days, preincubation with 15 mM mercaptoethanol restores the original activity Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.70 additional information pseudouridylate synthetase I is responsible for the biosynthesis of pseudouridine in the anticodon region of several tRNA species Salmonella enterica subsp. enterica serovar Typhimurium ?
-
?
4.2.1.70 uracil + D-ribose 5-phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium pseudouridine 5'-phosphate + H2O
-
?

Subunits

EC Number Subunits Comment Organism
4.2.1.70 dimer 2 * 50000, glycerol gradient sedimentation, tRNA induced dimerization Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.70 monomer 1* 50000 glycerol gradient sedimentation Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.70 0.005
-
tRNA wild-type RNA Salmonella enterica subsp. enterica serovar Typhimurium