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Literature summary extracted from

  • Fong, J.C.; Schulz, H.
    Purification and properties of pig heart crotonase and the presence of short chain and long chain enoyl coenzyme A hydratases in pig and guinea pig tissues (1977), J. Biol. Chem., 252, 542-547.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.17 acetoacetyl-CoA
-
 Sus scrofa
4.2.1.17 iodoacetamide 10 mM, 20 min, 20% inhibition Sus scrofa
4.2.1.17 NEM 10 mM, 19% inhibition. 5 mM, 13% inhibition Sus scrofa
4.2.1.17 p-chloromercuribenzoate 1 mM, complete inhibition. 0.1 mM, 11% inhibition Sus scrofa
4.2.1.74 additional information long chain enoyl-CoA hydratase of pig heart is not inhibited by acetoacetyl-CoA Cavia porcellus
4.2.1.74 additional information long chain enoyl-CoA hydratase of pig heart is not inhibited by acetoacetyl-CoA Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.17 0.013
-
 crotonyl-CoA pH 9.0 Sus scrofa
4.2.1.17 0.029
-
 hex-2-enoyl-CoA pH 9.0 Sus scrofa
4.2.1.17 0.029
-
 oct-2-enoyl-CoA pH 9.0 Sus scrofa
4.2.1.17 0.029
-
 dec-2-enoyl-CoA pH 9.0 Sus scrofa
4.2.1.17 0.03
-
 dodec-2-enoyl-CoA pH 9.0 Sus scrofa
4.2.1.17 0.03
-
 hexadec-2-enoyl-CoA pH 9.0 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.17 mitochondrion
-
 Sus scrofa 5739
-
4.2.1.74 mitochondrion
-
 Cavia porcellus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.17 27300
-
 6 * 27300, SDS-PAGE Sus scrofa
4.2.1.17 155000
-
 non-denaturing PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.17 Sus scrofa
-
 The classification is ambiguous because the stereochemistry of the reaction product is not exactly determined
-
4.2.1.74 Cavia porcellus
-
-
-
4.2.1.74 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.17
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.17 heart
-
 Sus scrofa
-
4.2.1.74 heart
-
 Cavia porcellus
-
4.2.1.74 heart
-
 Sus scrofa
-
4.2.1.74 liver
-
 Cavia porcellus
-
4.2.1.74 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.17 1334
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.17 crotonyl-CoA + H2O best substrate. The classification is ambiguous because the stereochemistry is not exactly determined Sus scrofa ?
-
 ?
4.2.1.17 dec-2-enoyl-CoA + H2O 32% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Sus scrofa ?
-
 ?
4.2.1.17 dodec-2-enoyl-CoA + H2O 9.6% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Sus scrofa ?
-
 ?
4.2.1.17 hex-2-enoyl-CoA + H2O 77% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Sus scrofa ?
-
 ?
4.2.1.17 hexadec-2-enoyl-CoA + H2O 2.4% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Sus scrofa ?
-
 ?
4.2.1.17 oct-2-enoyl-CoA + H2O 54% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.17 hexamer 6 * 27300, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
4.2.1.17 crotonase the classification is ambiguous because the stereochemistry of the reaction product is not exactly determined Sus scrofa
4.2.1.74 long chain enoyl coenzyme A hydratase
-
 Cavia porcellus
4.2.1.74 long chain enoyl coenzyme A hydratase
-
 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.17 8.5
-
-
 Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.17 0.014
-
 acetoacetyl-CoA pH 8.0, due to the binding of the enolate form of acetoacetyl-CoA the Ki-value should be highly pH-dependent Sus scrofa