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Literature summary extracted from

  • Suzuki, S.; Yamaguchi, J.; Tokushige, M.
    Purification and molecular properties of aspartate from Escherichia coli (1973), Biochim. Biophys. Acta, 321, 369-381.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.1 5,5'-dithiobis(2-nitrobenzoate) complete Escherichia coli
4.3.1.1 D-Aspartate competitive Escherichia coli
4.3.1.1 EDTA complete Escherichia coli
4.3.1.1 hydroxylamine competitive Escherichia coli
4.3.1.1 Na+ weak Escherichia coli
4.3.1.1 p-hydroxymercuribenzoate complete Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.1 1
-
L-aspartate
-
Escherichia coli
4.3.1.1 5
-
hydroxylamine substrate of the reverse reaction Escherichia coli
4.3.1.1 20
-
NH4+
-
Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.1 Ca2+ activates Escherichia coli
4.3.1.1 Cd2+ activates Escherichia coli
4.3.1.1 K+ activates Escherichia coli
4.3.1.1 Li+ activates Escherichia coli
4.3.1.1 Mg2+ activates Escherichia coli
4.3.1.1 Mn2+ activates Escherichia coli
4.3.1.1 additional information
-
Escherichia coli
4.3.1.1 Zn2+ activates Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.1 48500
-
4 * 48500, SDS-PAGE Escherichia coli
4.3.1.1 193000
-
sedimentation equilibrium analysis Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.1
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.1 additional information
-
-
Escherichia coli
4.3.1.1 68.5
-
-
Escherichia coli

Storage Stability

EC Number Storage Stability Organism
4.3.1.1 -20°C, stable for 1 month Escherichia coli
4.3.1.1 4C, in the presence of various salts thiol compounds, or glycerol stable for 2 weeks Escherichia coli
4.3.1.1 additional information Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.1 fumarate + hydroxylamine substrates for reverse reaction Escherichia coli ?
-
?
4.3.1.1 fumarate + NH4Cl substrates for reverse reaction Escherichia coli ?
-
?
4.3.1.1 L-aspartate absolutely specific Escherichia coli fumarate + NH3
-
?
4.3.1.1 L-aspartate natural substrate Escherichia coli fumarate + NH3
-
?

Subunits

EC Number Subunits Comment Organism
4.3.1.1 tetramer 4 * 48500, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.1 additional information
-
-
Escherichia coli
4.3.1.1 7.7
-
in the absence of MgCl2 Escherichia coli