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Literature summary extracted from

  • Tudball, N.; O'Neill, J.G.
    Substrate specificity of the L-serine O-sulphate degrading activities of Pseudomonas FR (1976), Biochim. Biophys. Acta, 429, 616-623.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.10 additional information
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.10 0.14
-
 D-serine O-sulfate enzyme B Pseudomonas sp.
4.3.1.10 0.17
-
 D-Cystine enzyme B Pseudomonas sp.
4.3.1.10 1.1
-
 S-Benzyl-L-cysteine enzyme A Pseudomonas sp.
4.3.1.10 1.3
-
 O-benzyl-L-serine enzyme A Pseudomonas sp.
4.3.1.10 2
-
 L-cystine enzyme A Pseudomonas sp.
4.3.1.10 2
-
 O-benzyl-DL-serine enzyme B, calculated on the basis of the non-reactivity of the L-isomer Pseudomonas sp.
4.3.1.10 2.2
-
 D-cystathionine enzyme B Pseudomonas sp.
4.3.1.10 5.4
-
 L-serine O-sulfate enzyme B Pseudomonas sp.
4.3.1.10 7.9
-
 L-serine O-sulfate enzyme A Pseudomonas sp.
4.3.1.10 8.3
-
 D-serine enzyme B Pseudomonas sp.
4.3.1.10 8.5
-
 L-cystathionine enzyme B Pseudomonas sp.
4.3.1.10 9.1
-
 L-cystathionine enzyme A Pseudomonas sp.
4.3.1.10 17.8
-
 S-methyl-L-cysteine enzyme A Pseudomonas sp.
4.3.1.10 20
-
 O-acetyl-L-serine enzyme A Pseudomonas sp.
4.3.1.10 38
-
 S-ethyl-L-cysteine enzyme A Pseudomonas sp.
4.3.1.10 56
-
 S-methyl-L-cysteine enzyme B Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.10 additional information Pseudomonas sp. Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B) ?
-
 ?
4.3.1.10 additional information Pseudomonas sp. FR Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B) ?
-
 ?
4.3.1.10 S-methyl-L-cysteine Pseudomonas sp. growth pattern is similar to that observed with L-serine O-sulfate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.10 Pseudomonas sp.
-
 FR
-
4.3.1.10 Pseudomonas sp. FR
-
 FR
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.10 enzyme A Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.10 additional information
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.10 3-chloro-L-alanine methyl ester 3-chloro-L-alanine Pseudomonas sp. ?
-
 ?
4.3.1.10 3-chloro-L-alanine methyl ester 3-chloro-L-alanine Pseudomonas sp. FR ?
-
 ?
4.3.1.10 3-cyano-L-alanine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 beta-ureido-alpha-aminopropionic acid
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 D-cystathionine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 D-cysteine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 D-cystine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 D-serine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 D-serine-O-sulfate
-
 Pseudomonas sp. pyruvate + NH3 + SO42-
-
 ?
4.3.1.10 DL-cysteine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 DL-lanthionine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 L-cystathionine + H2O
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 L-cysteine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 L-cystine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 L-serine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 L-serine O-sulfate
-
 Pseudomonas sp. pyruvate + NH3 + sulfate
-
 ?
4.3.1.10 L-serine O-sulfate
-
 Pseudomonas sp. FR pyruvate + NH3 + sulfate
-
 ?
4.3.1.10 L-threonine O-sulfate
-
 Pseudomonas sp. 2-oxobutanoate + NH3 + SO42-
-
 ?
4.3.1.10 L-threonine O-sulfate
-
 Pseudomonas sp. FR 2-oxobutanoate + NH3 + SO42-
-
 ?
4.3.1.10 additional information multi-substrate activity of the enzymes A and B with the enzyme A having a preferential activity for L-isomers and enzyme B for D-isomers Pseudomonas sp. ?
-
 ?
4.3.1.10 additional information Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B) Pseudomonas sp. ?
-
 ?
4.3.1.10 additional information multi-substrate activity of the enzymes A and B with the enzyme A having a preferential activity for L-isomers and enzyme B for D-isomers Pseudomonas sp. FR ?
-
 ?
4.3.1.10 additional information Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B) Pseudomonas sp. FR ?
-
 ?
4.3.1.10 O-acetyl-L-serine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 O-benzyl DL-serine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 O-benzyl-L-serine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 O-diazoacetyl-L-serine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 O-methyl DL-serine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 O-succinyl-L-serine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 S-benzyl-L-cysteine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 S-carbamoyl-L-cysteine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 S-ethyl-L-cysteine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 S-methyl L-cysteine
-
 Pseudomonas sp. ?
-
 ?
4.3.1.10 S-methyl-L-cysteine growth pattern is similar to that observed with L-serine O-sulfate Pseudomonas sp. ?
-
 ?
4.3.1.10 S-sulfo-L-cysteine
-
 Pseudomonas sp. ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.1.10 50
-
 enzyme B, after 15 min, 30% of the starting activity towards L-serine O-sulfate, 32% towards O-benzyl-DL-serine and 38% towards L-cystathionine. Enzyme A, after 15 min, 71% of the starting activity towards L-serine O-sulfate, 75% towards O-benzyl-DL-serine and 71% towards L-cystathionine Pseudomonas sp.