Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jeng, I.M.; Barker, H.A.
    Purification and properties of L-3-aminobutyryl coenzyme A deaminase from a lysine-fermenting Clostridium (1974), J. Biol. Chem., 249, 6578-6584.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.14 6,6'-Dithiodinicotinic acid
-
 Clostridium sp.
4.3.1.14 CaCl2 2 mM, 28% inhibition Clostridium sp.
4.3.1.14 iodoacetamide crotonyl-CoA protects Clostridium sp.
4.3.1.14 KCl 400 mM, 32% inhibition Clostridium sp.
4.3.1.14 MgCl2 2 mM, 22% inhibition Clostridium sp.
4.3.1.14 MgSO4 2 mM, 22% inhibition Clostridium sp.
4.3.1.14 NaCl 400 mM, 34% inhibition Clostridium sp.
4.3.1.14 NEM
-
 Clostridium sp.
4.3.1.14 PCMB
-
 Clostridium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.14 0.03
-
 L-3-aminobutyryl-CoA
-
 Clostridium sp.
4.3.1.14 0.045
-
 crotonyl-CoA
-
 Clostridium sp.
4.3.1.14 0.07
-
 NH3 with crotonyl-CoA as cosubstrate Clostridium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.14 64000
-
 gel filtration Clostridium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.14 additional information Clostridium sp. enzyme is involved in the pathway of Lys degradation ?
-
 ?
4.3.1.14 additional information Clostridium sp. SB4 enzyme is involved in the pathway of Lys degradation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.14 Clostridium sp.
-
 SB4
-
4.3.1.14 Clostridium sp. SB4
-
 SB4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.14
-
 Clostridium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.14 0.37
-
-
 Clostridium sp.

Storage Stability

EC Number Storage Stability Organism
4.3.1.14 -20°C, 3 mg/ml enzyme concentration, 4% loss of activity in 3 months Clostridium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.14 crotonoyl-CoA + NH3 NH3 in form of NH4Cl Clostridium sp. L-3-aminobutyryl-CoA
-
 ?
4.3.1.14 Crotonyl pantetheine + NH3 aminated 100% as fast as crotonyl-CoA Clostridium sp. Aminobutanoylpantetheine
-
 ?
4.3.1.14 Crotonyl pantetheine + NH3 aminated 100% as fast as crotonyl-CoA Clostridium sp. SB4 Aminobutanoylpantetheine
-
 ?
4.3.1.14 Crotonyl-CoA + hydroxylamine
-
 Clostridium sp. CoA + 3-methyl-5-isoxazolidinone
-
 ?
4.3.1.14 Crotonyl-CoA + hydroxylamine
-
 Clostridium sp. SB4 CoA + 3-methyl-5-isoxazolidinone
-
 ?
4.3.1.14 L-3-Aminobutanoyl-CoA r Clostridium sp. Crotonyl-CoA + NH3
-
 ?
4.3.1.14 L-3-Aminobutanoyl-CoA only active with the L-isomer Clostridium sp. Crotonyl-CoA + NH3
-
 ?
4.3.1.14 L-3-Aminobutanoyl-CoA r Clostridium sp. SB4 Crotonyl-CoA + NH3
-
 ?
4.3.1.14 L-3-Aminobutanoyl-CoA only active with the L-isomer Clostridium sp. SB4 Crotonyl-CoA + NH3
-
 ?
4.3.1.14 L-3-aminobutyryl-CoA
-
 Clostridium sp. crotonoyl-CoA + NH3
-
 ?
4.3.1.14 additional information enzyme is involved in the pathway of Lys degradation Clostridium sp. ?
-
 ?
4.3.1.14 additional information enzyme is involved in the pathway of Lys degradation Clostridium sp. SB4 ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.1.14 37
-
 pH 6.8, half-life: 7.5 h, 0.05 mg of protein per ml Clostridium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.14 9.2
-
 amination Clostridium sp.
4.3.1.14 9.7
-
 deamination Clostridium sp.