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Literature summary extracted from

  • Harms, C.; Ludwig, U.; Andressen, J.R.
    Sarcosine reductase of Tissierella creatinophila: purification and characterization of its components (1998), Arch. Microbiol., 170, 442-450.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.21.4.3 21000
-
 protein A, SDS-PAGE Tissierella creatinophila
1.21.4.3 45000
-
 protein A, gel filtration Tissierella creatinophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.4.3 N-methylglycine + phosphate + thioredoxin Bacillus subtilis
-
 acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.3 N-methylglycine + phosphate + thioredoxin Clostridium pasteurianum
-
 acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.3 N-methylglycine + phosphate + thioredoxin Azotobacter vinelandii
-
 acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.3 N-methylglycine + phosphate + thioredoxin Clostridium sporogenes
-
 acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.3 N-methylglycine + phosphate + thioredoxin Peptoclostridium acidaminophilum
-
 acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.3 N-methylglycine + phosphate + thioredoxin Peptoclostridium litorale
-
 acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.3 N-methylglycine + phosphate + thioredoxin Tissierella creatinophila
-
 acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.4.3 Azotobacter vinelandii
-
 DSM 87, ATCC 12837
-
1.21.4.3 Bacillus subtilis
-
 DSM 402
-
1.21.4.3 Clostridium pasteurianum
-
 DSM 525, ATCC 6013
-
1.21.4.3 Clostridium sporogenes
-
 DSM 633
-
1.21.4.3 Peptoclostridium acidaminophilum
-
 DSM 3953
-
1.21.4.3 Peptoclostridium litorale
-
-
-
1.21.4.3 Tissierella creatinophila
-
 DSM 6911, grown on creatine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.4.3 components A, B and C of enzyme complex purified separately Peptoclostridium acidaminophilum
1.21.4.3 components A, B and C of enzyme complex purified separately Tissierella creatinophila

Storage Stability

EC Number Storage Stability Organism
1.21.4.3 -20°C, stable for several weeks Tissierella creatinophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.4.3 N-methylglycine + phosphate + thioredoxin
-
 Bacillus subtilis acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.3 N-methylglycine + phosphate + thioredoxin
-
 Clostridium pasteurianum acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.3 N-methylglycine + phosphate + thioredoxin
-
 Azotobacter vinelandii acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.3 N-methylglycine + phosphate + thioredoxin
-
 Clostridium sporogenes acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.3 N-methylglycine + phosphate + thioredoxin
-
 Peptoclostridium acidaminophilum acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.3 N-methylglycine + phosphate + thioredoxin
-
 Peptoclostridium litorale acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.3 N-methylglycine + phosphate + thioredoxin
-
 Tissierella creatinophila acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?

Subunits

EC Number Subunits Comment Organism
1.21.4.3 dimer 2 * 21000, gel filtration, SDS-PAGE Tissierella creatinophila

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.21.4.3 4.9
-
 complete loss of activity within 24 h Tissierella creatinophila
1.21.4.3 5.2
-
 38% loss of activity within 24 h Tissierella creatinophila
1.21.4.3 5.4 5.8 10% loss of activity within 24 h Tissierella creatinophila