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Literature summary extracted from

  • McGregor, W.G.; Philips, J.; Suelter, C.H.
    Purification and kinetic characterization of a monovalent cation-activated glycerol dehydrogenase from Aerobacter aerogenes (1974), J. Biol. Chem., 249, 3132-3139.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.6 1,10-phenanthroline at 1.85 mM 135% of the activity without activator Klebsiella aerogenes
1.1.1.6 2-mercaptoethanol 1 mM, slight activation Klebsiella aerogenes
1.1.1.6 8-Quinolinol 1 mM, slight activation Klebsiella aerogenes
1.1.1.6 dithioerythritol 1 mM, slight activation Klebsiella aerogenes
1.1.1.6 NH4+
-
 Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.6 2-mercaptoethanol strongly inhibitory at 100 mM, but slight activation in lower concentration, 1 mM Klebsiella aerogenes
1.1.1.6 8-Quinolinol 1 mM, 10 mM Klebsiella aerogenes
1.1.1.6 dithioerythritol 10 mM Klebsiella aerogenes
1.1.1.6 EDTA 3 mM, 30 mM Klebsiella aerogenes
1.1.1.6 iodoacetamide strong inhibition, 10 mM Klebsiella aerogenes
1.1.1.6 N-ethylmaleimide
-
 Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.6 74.3
-
 glycerol in the presence of NH4+ Klebsiella aerogenes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.6 cytoplasm soluble, isolation from crude cell extract Klebsiella aerogenes 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.6 K+ activates Klebsiella aerogenes
1.1.1.6 Rb+ activates Klebsiella aerogenes
1.1.1.6 Tl+ activates Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.6 Klebsiella aerogenes
-
-
-
1.1.1.6 Klebsiella aerogenes 1033
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.6
-
 Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.6 35
-
-
 Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.6 glycerol + NAD+
-
 Klebsiella aerogenes dihydroxyacetone + NADH + H+
-
 ?
1.1.1.6 glycerol + NAD+
-
 Klebsiella aerogenes 1033 dihydroxyacetone + NADH + H+
-
 ?