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Literature summary extracted from

  • Fang, J.; Han, Q.; Li, J.
    Isolation, characterization, and functional expression of kynurenine aminotransferase cDNA from the yellow fever mosquito, Aedes aegypti(1) (2002), Insect Biochem. Mol. Biol., 32, 943-950.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.7 expression of full length enzyme and truncated version lacking the mitochondrial leader sequence in Sf9 insect cells Aedes aegypti

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.7 mitochondrion deduced amino acid sequence contains a mitochondrial leader sequence Aedes aegypti 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.7 48000
-
2 * 48000, truncated recombinant enzyme, SDS-PAGE Aedes aegypti
2.6.1.7 53000
-
x * 53000, SDS-PAGE Aedes aegypti
2.6.1.7 53490
-
x * 53490, deduced from nucleotide sequence Aedes aegypti
2.6.1.7 97000
-
truncated recombinant enzyme, gel filtration Aedes aegypti

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.7 Aedes aegypti Q95VY4 yellow fever mosquito
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.7 truncated recombinant enzyme, DEAE-Sepharose, native PAGE Aedes aegypti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.7 L-kynurenine + 2-oxoadipate 25% of activity with pyruvate Aedes aegypti kynurenic acid + 2-aminoadipate + H2O
-
?
2.6.1.7 L-kynurenine + 2-oxoglutarate 31% of activity with pyruvate Aedes aegypti kynurenic acid + L-glutamate + H2O
-
?
2.6.1.7 L-kynurenine + oxaloacetate 90% of activity with pyruvate Aedes aegypti kynurenic acid + L-aspartate + H2O
-
?
2.6.1.7 L-kynurenine + pyruvate best amino acceptor Aedes aegypti kynurenic acid + L-alanine + H2O
-
?

Subunits

EC Number Subunits Comment Organism
2.6.1.7 ? x * 53000, SDS-PAGE Aedes aegypti
2.6.1.7 ? x * 53490, deduced from nucleotide sequence Aedes aegypti
2.6.1.7 dimer 2 * 48000, truncated recombinant enzyme, SDS-PAGE Aedes aegypti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.7 60
-
recombinant enzyme Aedes aegypti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.7 8.5
-
optima at pH 8.5 and 10.0 Aedes aegypti
2.6.1.7 10
-
optima at pH 8.5 and 10.0 Aedes aegypti