EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.1 | 2-oxoglutarate | in cell extracts, alanine activity is inhibited by 50% by about 2.5 mM 2-oxoglutarate, while the aspartate activity is unaffected at this concentration. The highly purified enzyme is also inhibited by 2-oxoglutarate, but the extent of inhibition is slightly more than that in extracts. Thus, alanine activity is completely inhibited by 10 mM 2-oxoglutarate, and 36% of the aspartate activity is inhibited | Methanococcus aeolicus | |
2.6.1.1 | L-aspartate | inhibits tyrosine transamination | Methanococcus aeolicus | |
2.6.1.1 | L-cysteine | 10 mM, 30% inactivation | Methanococcus aeolicus | |
2.6.1.1 | L-histidine | inhibition is relieved by high concentrations of substrates | Methanococcus aeolicus | |
2.6.1.1 | additional information | the activities with 2 mM aspartate or alanine are not inhibited by 10 mM isoleucine, phenylalanine, arginine, asparagine, glutamine, glycine, lysine, methionine, proline, serine, and threonine | Methanococcus aeolicus | |
2.6.1.42 | 2-oxoglutarate | 5 mM 2-oxoglutarate inhibits the transamination of the 6.3-fold purified enzyme, of valine and isoleucine by about 50%, but the leucine activity is less affected. Inhibition is competitive with respect to valine | Methanococcus aeolicus | |
2.6.1.42 | L-cysteine | 10 mM, inhibits activity with isoleucine and tyrosine by 22 and 34%, respectively | Methanococcus aeolicus | |
2.6.1.42 | L-isoleucine | with 1 mM tyrosine as the substrate, 10 mM isoleucine inhibits activity by 94% | Methanococcus aeolicus | |
2.6.1.42 | L-leucine | with 1 mM tyrosine as the substrate, 10 mM leucine inhibits activity by 93% | Methanococcus aeolicus | |
2.6.1.42 | L-methionine | 10 mM methionine inhibits the activity with 1 mM tyrosine and phenylalanine by 86 and 59%, respectively, but it has no effect on the isoleucine activity | Methanococcus aeolicus | |
2.6.1.42 | L-phenylalanine | with 2 mM isoleucine as the substrate, 10 mM phenylalanine inhibits activity by 46% | Methanococcus aeolicus | |
2.6.1.42 | L-valine | with 1 mM tyrosine as the substrate, 10 mM valine inhibits activity by 78% | Methanococcus aeolicus | |
2.6.1.42 | additional information | not inhibited by 5 mM KCI, NaCl, CuCl2, NiCl2, CoCl2, or FeCl2. No of activity with tyrosine by 10 mM aspartate, arginine, asparagine, glutamine, glycine, histidine, lysine, proline, serine, threonine or alanine | Methanococcus aeolicus | |
2.6.1.42 | ZnCl2 | 5 mM, 50% inhibition | Methanococcus aeolicus | |
2.6.1.57 | cysteine | - |
Methanococcus aeolicus | |
2.6.1.57 | L-cysteine | 10 mM | Methanococcus aeolicus | |
2.6.1.57 | methionine | 10 mM, inhibits activity of isoenzyme ArAT-I with 1 mM tyrosine by 23%, isoenzyme ArAT-II is not affected | Methanococcus aeolicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.1 | 0.2 | - |
2-oxoglutarate | pH 7.5, 37°C | Methanococcus aeolicus | |
2.6.1.1 | 0.5 | - |
2-oxoglutarate | pH 7.5, 37°C | Methanococcus aeolicus | |
2.6.1.1 | 3.5 | - |
L-alanine | pH 7.5, 37°C | Methanococcus aeolicus | |
2.6.1.1 | 3.7 | - |
L-aspartate | pH 7.5, 37°C | Methanococcus aeolicus | |
2.6.1.42 | 0.1 | - |
L-phenylalanine | pH and temperature not specified in the publication | Methanococcus aeolicus | |
2.6.1.42 | 0.1 | - |
2-oxoglutarate | pH and temperature not specified in the publication, cosubstrate: L-phenylalanine | Methanococcus aeolicus | |
2.6.1.42 | 0.1 | - |
2-oxoglutarate | pH and temperature not specified in the publication, cosubstrate: L-tryptophan | Methanococcus aeolicus | |
2.6.1.42 | 0.1 | - |
2-oxoglutarate | pH and temperature not specified in the publication, cosubstrate: L-tyrosine | Methanococcus aeolicus | |
2.6.1.42 | 0.3 | - |
2-oxoglutarate | pH and temperature not specified in the publication, cosubstrate: L-isoleucine | Methanococcus aeolicus | |
2.6.1.42 | 0.3 | - |
2-oxoglutarate | pH and temperature not specified in the publication, cosubstrate: L-valine | Methanococcus aeolicus | |
2.6.1.42 | 0.6 | - |
2-oxoglutarate | pH and temperature not specified in the publication, cosubstrate: L-leucine | Methanococcus aeolicus | |
2.6.1.42 | 1.1 | - |
L-tyrosine | pH and temperature not specified in the publication | Methanococcus aeolicus | |
2.6.1.42 | 1.1 | - |
L-leucine | pH and temperature not specified in the publication | Methanococcus aeolicus | |
2.6.1.42 | 1.4 | - |
L-isoleucine | pH and temperature not specified in the publication | Methanococcus aeolicus | |
2.6.1.42 | 2.8 | - |
L-tryptophan | pH and temperature not specified in the publication | Methanococcus aeolicus | |
2.6.1.42 | 2.8 | - |
L-valine | pH and temperature not specified in the publication | Methanococcus aeolicus | |
2.6.1.57 | 0.3 | - |
2-oxoglutarate | pH 7.5, 37°C, cosubstrate: L-tyrosine, aromatic aminotransferase II | Methanococcus aeolicus | |
2.6.1.57 | 0.4 | - |
L-phenylalanine | pH 7.5, 37°C, aromatic aminotransferase I | Methanococcus aeolicus | |
2.6.1.57 | 0.4 | - |
2-oxoglutarate | pH 7.5, 37°C, cosubstrate: L-tyrosine, aromatic aminotransferase I | Methanococcus aeolicus | |
2.6.1.57 | 0.6 | - |
2-oxoglutarate | pH 7.5, 37°C, cosubstrate: L-phenylalanine, aromatic aminotransferase I | Methanococcus aeolicus | |
2.6.1.57 | 0.6 | - |
2-oxoglutarate | pH 7.5, 37°C, cosubstrate: L-phenylalanine, aromatic aminotransferase II | Methanococcus aeolicus | |
2.6.1.57 | 1 | - |
L-tyrosine | pH 7.5, 37°C, aromatic aminotransferase I | Methanococcus aeolicus | |
2.6.1.57 | 1 | - |
L-tryptophan | pH 7.5, 37°C, aromatic aminotransferase I | Methanococcus aeolicus | |
2.6.1.57 | 1.2 | - |
L-phenylalanine | pH 7.5, 37°C, aromatic aminotransferase II | Methanococcus aeolicus | |
2.6.1.57 | 1.8 | - |
2-oxoglutarate | pH 7.5, 37°C, cosubstrate: L-tryptophan, aromatic aminotransferase I | Methanococcus aeolicus | |
2.6.1.57 | 1.9 | - |
L-tryptophan | pH 7.5, 37°C, aromatic aminotransferase II | Methanococcus aeolicus | |
2.6.1.57 | 2.1 | - |
L-tyrosine | pH 7.5, 37°C, aromatic aminotransferase II | Methanococcus aeolicus | |
2.6.1.57 | 4.6 | - |
2-oxoglutarate | pH 7.5, 37°C, cosubstrate: L-tryptophan, aromatic aminotransferase II | Methanococcus aeolicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.42 | Mg2+ | 5 mM MgCl2 stimulates by 26% | Methanococcus aeolicus | |
2.6.1.42 | Mn2+ | 5 mM MgCl2 stimulates by 34% | Methanococcus aeolicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 162000 | - |
gel filtration | Methanococcus aeolicus |
2.6.1.42 | 188000 | - |
gel filtration | Methanococcus aeolicus |
2.6.1.57 | 90000 | - |
ArAT-II, gel filtration | Methanococcus aeolicus |
2.6.1.57 | 150000 | - |
ArAT-I, gel filtration | Methanococcus aeolicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.1 | L-aspartate + 2-oxoglutarate | Methanococcus aeolicus | - |
oxaloacetate + L-glutamate | - |
? | |
2.6.1.57 | L-phenylalanine + 2-oxoglutarate | Methanococcus aeolicus | - |
phenylpyruvate + L-glutamate | - |
? | |
2.6.1.57 | L-tryptophan + 2-oxoglutarate | Methanococcus aeolicus | - |
3-indole-2-oxopropanoate + L-glutamate | - |
? | |
2.6.1.57 | L-tyrosine + 2-oxoglutarate | Methanococcus aeolicus | - |
p-hydroxyphenylpyruvate + L-glutamate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.1 | Methanococcus aeolicus | - |
- |
- |
2.6.1.42 | Methanococcus aeolicus | - |
- |
- |
2.6.1.57 | Methanococcus aeolicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.1 | partial | Methanococcus aeolicus |
2.6.1.42 | partial | Methanococcus aeolicus |
2.6.1.57 | partial | Methanococcus aeolicus |
2.6.1.57 | partial purification of enzyme forms ArAT-I and ArAT-II | Methanococcus aeolicus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 0.3 | - |
pH 7.5, 37°C, enzyme in crude extract | Methanococcus aeolicus |
2.6.1.1 | 25.3 | - |
pH 7.5, 37°C, partiall purified enzyme | Methanococcus aeolicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.1 | L-alanine + 2-oxoglutarate | 0.7% of the activity with L-aspartate | Methanococcus aeolicus | pyruvate + L-glutamate | - |
? | |
2.6.1.1 | L-aspartate + 2-oxoglutarate | - |
Methanococcus aeolicus | oxaloacetate + L-glutamate | - |
? | |
2.6.1.1 | L-phenylalanine + 2-oxoglutarate | 0.07% of the activity with L-aspartate | Methanococcus aeolicus | 2-oxo-3-phenylpropionic acid + L-glutamate | - |
? | |
2.6.1.1 | L-tryptophan + 2-oxoglutarate | 0.08% of the activity with aspartate | Methanococcus aeolicus | 3-indole-2-oxopropionic acid + L-glutamate | - |
? | |
2.6.1.1 | L-tryptophan + 2-oxoglutarate | 0.08% of the activity with L-aspartate | Methanococcus aeolicus | 3-indole-2-oxopropionic acid + L-glutamate | - |
? | |
2.6.1.1 | L-tyrosine + 2-oxoglutarate | 0.04% of the activity with aspartate | Methanococcus aeolicus | 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate | i.e. 4-hydroxyphenylpyruvate | ? | |
2.6.1.1 | L-tyrosine + 2-oxoglutarate | 0.04% of the activity with L-aspartate | Methanococcus aeolicus | 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate | - |
? | |
2.6.1.42 | L-isoleucine + 2-oxoglutarate | - |
Methanococcus aeolicus | L-glutamate + 3-methyl-2-oxopentanoate | - |
? | |
2.6.1.42 | L-leucine + 2-oxoglutarate | - |
Methanococcus aeolicus | 4-methyl-2-oxopentanoate + L-glutamate | - |
? | |
2.6.1.42 | L-phenylalanine + 2-oxoglutarate | - |
Methanococcus aeolicus | phenylpyruvate + L-glutamate | - |
? | |
2.6.1.42 | L-tryptophan + 2-oxoglutarate | - |
Methanococcus aeolicus | L-glutamic acid + 3-indole-2-oxopropanoate | - |
? | |
2.6.1.42 | L-tyrosine + 2-oxoglutarate | - |
Methanococcus aeolicus | p-hydroxyphenylpyruvate + L-glutamate | - |
? | |
2.6.1.42 | L-valine + 2-oxoglutarate | - |
Methanococcus aeolicus | 3-methyl-2-oxobutanoate + L-glutamate | - |
? | |
2.6.1.57 | L-phenylalanine + 2-oxoglutarate | - |
Methanococcus aeolicus | phenylpyruvate + L-glutamate | - |
? | |
2.6.1.57 | L-tryptophan + 2-oxoglutarate | - |
Methanococcus aeolicus | 3-indole-2-oxopropanoate + L-glutamate | - |
? | |
2.6.1.57 | L-tyrosine + 2-oxoglutarate | - |
Methanococcus aeolicus | p-hydroxyphenylpyruvate + L-glutamate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.1 | aspartate aminotransferase | - |
Methanococcus aeolicus |
2.6.1.1 | AspAT | - |
Methanococcus aeolicus |
2.6.1.42 | BcaT | - |
Methanococcus aeolicus |
2.6.1.42 | branched-chain aminotransferase | - |
Methanococcus aeolicus |
2.6.1.57 | ArAT-I | - |
Methanococcus aeolicus |
2.6.1.57 | aromatic aminotransferase | - |
Methanococcus aeolicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 37 | - |
assay at | Methanococcus aeolicus |
2.6.1.57 | 37 | - |
assay at | Methanococcus aeolicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 7.5 | - |
assay at | Methanococcus aeolicus |
2.6.1.42 | 7.5 | - |
- |
Methanococcus aeolicus |
2.6.1.57 | 7.5 | - |
assay at | Methanococcus aeolicus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.42 | 1.9 | - |
2-oxoglutarate | 6.3-fold purified enzyme, pH and temperature not specified in the publication | Methanococcus aeolicus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.6.1.1 | Methanococcus aeolicus | isoelectric focusing | - |
4.4 |
2.6.1.42 | Methanococcus aeolicus | isoelectric focusing | - |
4.9 |