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Literature summary extracted from

  • Xing, R.; Whitman, W.B.
    Characterization of amino acid aminotransferases of Methanococcus aeolicus (1974), J. Bacteriol., 174, 541-548.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.1 2-oxoglutarate in cell extracts, alanine activity is inhibited by 50% by about 2.5 mM 2-oxoglutarate, while the aspartate activity is unaffected at this concentration. The highly purified enzyme is also inhibited by 2-oxoglutarate, but the extent of inhibition is slightly more than that in extracts. Thus, alanine activity is completely inhibited by 10 mM 2-oxoglutarate, and 36% of the aspartate activity is inhibited Methanococcus aeolicus
2.6.1.1 L-aspartate inhibits tyrosine transamination Methanococcus aeolicus
2.6.1.1 L-cysteine 10 mM, 30% inactivation Methanococcus aeolicus
2.6.1.1 L-histidine inhibition is relieved by high concentrations of substrates Methanococcus aeolicus
2.6.1.1 additional information the activities with 2 mM aspartate or alanine are not inhibited by 10 mM isoleucine, phenylalanine, arginine, asparagine, glutamine, glycine, lysine, methionine, proline, serine, and threonine Methanococcus aeolicus
2.6.1.42 2-oxoglutarate 5 mM 2-oxoglutarate inhibits the transamination of the 6.3-fold purified enzyme, of valine and isoleucine by about 50%, but the leucine activity is less affected. Inhibition is competitive with respect to valine Methanococcus aeolicus
2.6.1.42 L-cysteine 10 mM, inhibits activity with isoleucine and tyrosine by 22 and 34%, respectively Methanococcus aeolicus
2.6.1.42 L-isoleucine with 1 mM tyrosine as the substrate, 10 mM isoleucine inhibits activity by 94% Methanococcus aeolicus
2.6.1.42 L-leucine with 1 mM tyrosine as the substrate, 10 mM leucine inhibits activity by 93% Methanococcus aeolicus
2.6.1.42 L-methionine 10 mM methionine inhibits the activity with 1 mM tyrosine and phenylalanine by 86 and 59%, respectively, but it has no effect on the isoleucine activity Methanococcus aeolicus
2.6.1.42 L-phenylalanine with 2 mM isoleucine as the substrate, 10 mM phenylalanine inhibits activity by 46% Methanococcus aeolicus
2.6.1.42 L-valine with 1 mM tyrosine as the substrate, 10 mM valine inhibits activity by 78% Methanococcus aeolicus
2.6.1.42 additional information not inhibited by 5 mM KCI, NaCl, CuCl2, NiCl2, CoCl2, or FeCl2. No of activity with tyrosine by 10 mM aspartate, arginine, asparagine, glutamine, glycine, histidine, lysine, proline, serine, threonine or alanine Methanococcus aeolicus
2.6.1.42 ZnCl2 5 mM, 50% inhibition Methanococcus aeolicus
2.6.1.57 cysteine
-
Methanococcus aeolicus
2.6.1.57 L-cysteine 10 mM Methanococcus aeolicus
2.6.1.57 methionine 10 mM, inhibits activity of isoenzyme ArAT-I with 1 mM tyrosine by 23%, isoenzyme ArAT-II is not affected Methanococcus aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.1 0.2
-
2-oxoglutarate pH 7.5, 37°C Methanococcus aeolicus
2.6.1.1 0.5
-
2-oxoglutarate pH 7.5, 37°C Methanococcus aeolicus
2.6.1.1 3.5
-
L-alanine pH 7.5, 37°C Methanococcus aeolicus
2.6.1.1 3.7
-
L-aspartate pH 7.5, 37°C Methanococcus aeolicus
2.6.1.42 0.1
-
L-phenylalanine pH and temperature not specified in the publication Methanococcus aeolicus
2.6.1.42 0.1
-
2-oxoglutarate pH and temperature not specified in the publication, cosubstrate: L-phenylalanine Methanococcus aeolicus
2.6.1.42 0.1
-
2-oxoglutarate pH and temperature not specified in the publication, cosubstrate: L-tryptophan Methanococcus aeolicus
2.6.1.42 0.1
-
2-oxoglutarate pH and temperature not specified in the publication, cosubstrate: L-tyrosine Methanococcus aeolicus
2.6.1.42 0.3
-
2-oxoglutarate pH and temperature not specified in the publication, cosubstrate: L-isoleucine Methanococcus aeolicus
2.6.1.42 0.3
-
2-oxoglutarate pH and temperature not specified in the publication, cosubstrate: L-valine Methanococcus aeolicus
2.6.1.42 0.6
-
2-oxoglutarate pH and temperature not specified in the publication, cosubstrate: L-leucine Methanococcus aeolicus
2.6.1.42 1.1
-
L-tyrosine pH and temperature not specified in the publication Methanococcus aeolicus
2.6.1.42 1.1
-
L-leucine pH and temperature not specified in the publication Methanococcus aeolicus
2.6.1.42 1.4
-
L-isoleucine pH and temperature not specified in the publication Methanococcus aeolicus
2.6.1.42 2.8
-
L-tryptophan pH and temperature not specified in the publication Methanococcus aeolicus
2.6.1.42 2.8
-
L-valine pH and temperature not specified in the publication Methanococcus aeolicus
2.6.1.57 0.3
-
2-oxoglutarate pH 7.5, 37°C, cosubstrate: L-tyrosine, aromatic aminotransferase II Methanococcus aeolicus
2.6.1.57 0.4
-
L-phenylalanine pH 7.5, 37°C, aromatic aminotransferase I Methanococcus aeolicus
2.6.1.57 0.4
-
2-oxoglutarate pH 7.5, 37°C, cosubstrate: L-tyrosine, aromatic aminotransferase I Methanococcus aeolicus
2.6.1.57 0.6
-
2-oxoglutarate pH 7.5, 37°C, cosubstrate: L-phenylalanine, aromatic aminotransferase I Methanococcus aeolicus
2.6.1.57 0.6
-
2-oxoglutarate pH 7.5, 37°C, cosubstrate: L-phenylalanine, aromatic aminotransferase II Methanococcus aeolicus
2.6.1.57 1
-
L-tyrosine pH 7.5, 37°C, aromatic aminotransferase I Methanococcus aeolicus
2.6.1.57 1
-
L-tryptophan pH 7.5, 37°C, aromatic aminotransferase I Methanococcus aeolicus
2.6.1.57 1.2
-
L-phenylalanine pH 7.5, 37°C, aromatic aminotransferase II Methanococcus aeolicus
2.6.1.57 1.8
-
2-oxoglutarate pH 7.5, 37°C, cosubstrate: L-tryptophan, aromatic aminotransferase I Methanococcus aeolicus
2.6.1.57 1.9
-
L-tryptophan pH 7.5, 37°C, aromatic aminotransferase II Methanococcus aeolicus
2.6.1.57 2.1
-
L-tyrosine pH 7.5, 37°C, aromatic aminotransferase II Methanococcus aeolicus
2.6.1.57 4.6
-
2-oxoglutarate pH 7.5, 37°C, cosubstrate: L-tryptophan, aromatic aminotransferase II Methanococcus aeolicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.6.1.42 Mg2+ 5 mM MgCl2 stimulates by 26% Methanococcus aeolicus
2.6.1.42 Mn2+ 5 mM MgCl2 stimulates by 34% Methanococcus aeolicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.1 162000
-
gel filtration Methanococcus aeolicus
2.6.1.42 188000
-
gel filtration Methanococcus aeolicus
2.6.1.57 90000
-
ArAT-II, gel filtration Methanococcus aeolicus
2.6.1.57 150000
-
ArAT-I, gel filtration Methanococcus aeolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate Methanococcus aeolicus
-
oxaloacetate + L-glutamate
-
?
2.6.1.57 L-phenylalanine + 2-oxoglutarate Methanococcus aeolicus
-
phenylpyruvate + L-glutamate
-
?
2.6.1.57 L-tryptophan + 2-oxoglutarate Methanococcus aeolicus
-
3-indole-2-oxopropanoate + L-glutamate
-
?
2.6.1.57 L-tyrosine + 2-oxoglutarate Methanococcus aeolicus
-
p-hydroxyphenylpyruvate + L-glutamate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 Methanococcus aeolicus
-
-
-
2.6.1.42 Methanococcus aeolicus
-
-
-
2.6.1.57 Methanococcus aeolicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.1 partial Methanococcus aeolicus
2.6.1.42 partial Methanococcus aeolicus
2.6.1.57 partial Methanococcus aeolicus
2.6.1.57 partial purification of enzyme forms ArAT-I and ArAT-II Methanococcus aeolicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.1 0.3
-
pH 7.5, 37°C, enzyme in crude extract Methanococcus aeolicus
2.6.1.1 25.3
-
pH 7.5, 37°C, partiall purified enzyme Methanococcus aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 L-alanine + 2-oxoglutarate 0.7% of the activity with L-aspartate Methanococcus aeolicus pyruvate + L-glutamate
-
?
2.6.1.1 L-aspartate + 2-oxoglutarate
-
Methanococcus aeolicus oxaloacetate + L-glutamate
-
?
2.6.1.1 L-phenylalanine + 2-oxoglutarate 0.07% of the activity with L-aspartate Methanococcus aeolicus 2-oxo-3-phenylpropionic acid + L-glutamate
-
?
2.6.1.1 L-tryptophan + 2-oxoglutarate 0.08% of the activity with aspartate Methanococcus aeolicus 3-indole-2-oxopropionic acid + L-glutamate
-
?
2.6.1.1 L-tryptophan + 2-oxoglutarate 0.08% of the activity with L-aspartate Methanococcus aeolicus 3-indole-2-oxopropionic acid + L-glutamate
-
?
2.6.1.1 L-tyrosine + 2-oxoglutarate 0.04% of the activity with aspartate Methanococcus aeolicus 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate i.e. 4-hydroxyphenylpyruvate ?
2.6.1.1 L-tyrosine + 2-oxoglutarate 0.04% of the activity with L-aspartate Methanococcus aeolicus 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate
-
?
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
Methanococcus aeolicus L-glutamate + 3-methyl-2-oxopentanoate
-
?
2.6.1.42 L-leucine + 2-oxoglutarate
-
Methanococcus aeolicus 4-methyl-2-oxopentanoate + L-glutamate
-
?
2.6.1.42 L-phenylalanine + 2-oxoglutarate
-
Methanococcus aeolicus phenylpyruvate + L-glutamate
-
?
2.6.1.42 L-tryptophan + 2-oxoglutarate
-
Methanococcus aeolicus L-glutamic acid + 3-indole-2-oxopropanoate
-
?
2.6.1.42 L-tyrosine + 2-oxoglutarate
-
Methanococcus aeolicus p-hydroxyphenylpyruvate + L-glutamate
-
?
2.6.1.42 L-valine + 2-oxoglutarate
-
Methanococcus aeolicus 3-methyl-2-oxobutanoate + L-glutamate
-
?
2.6.1.57 L-phenylalanine + 2-oxoglutarate
-
Methanococcus aeolicus phenylpyruvate + L-glutamate
-
?
2.6.1.57 L-tryptophan + 2-oxoglutarate
-
Methanococcus aeolicus 3-indole-2-oxopropanoate + L-glutamate
-
?
2.6.1.57 L-tyrosine + 2-oxoglutarate
-
Methanococcus aeolicus p-hydroxyphenylpyruvate + L-glutamate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.1 aspartate aminotransferase
-
Methanococcus aeolicus
2.6.1.1 AspAT
-
Methanococcus aeolicus
2.6.1.42 BcaT
-
Methanococcus aeolicus
2.6.1.42 branched-chain aminotransferase
-
Methanococcus aeolicus
2.6.1.57 ArAT-I
-
Methanococcus aeolicus
2.6.1.57 aromatic aminotransferase
-
Methanococcus aeolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.1 37
-
assay at Methanococcus aeolicus
2.6.1.57 37
-
assay at Methanococcus aeolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.1 7.5
-
assay at Methanococcus aeolicus
2.6.1.42 7.5
-
-
Methanococcus aeolicus
2.6.1.57 7.5
-
assay at Methanococcus aeolicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.42 1.9
-
2-oxoglutarate 6.3-fold purified enzyme, pH and temperature not specified in the publication Methanococcus aeolicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.6.1.1 Methanococcus aeolicus isoelectric focusing
-
4.4
2.6.1.42 Methanococcus aeolicus isoelectric focusing
-
4.9