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Literature summary extracted from

  • Carpe, A.I.; Smith, I.K.
    Serine-glyoxylate aminotransferase from kidney bean (Phaseolus vulgaris). II. The reverse reactions (1974), Biochim. Biophys. Acta, 370, 96-101.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.45 hydroxylamine 0.01 mM, 85% inhibition of glycin-hydroxypyruvate transamination Phaseolus vulgaris
2.6.1.45 N-ethylmaleimide
-
 Phaseolus vulgaris
2.6.1.45 NH4Cl glycine-hydroxypyruvate transamination is only weakly inhibited Phaseolus vulgaris
2.6.1.45 p-hydroxymercuribenzoate
-
 Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.45 0.63
-
 3-hydroxypyruvate pH 8.0, 30°C, cosubstrate L-alanine Phaseolus vulgaris
2.6.1.45 1.1
-
 3-hydroxypyruvate pH 8.0, 30°C, cosubstrate glycine Phaseolus vulgaris
2.6.1.45 11
-
 glycine pH 8.0, 30°C Phaseolus vulgaris
2.6.1.45 20
-
 L-alanine pH 8.0, 30°C Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.45 L-serine + glyoxylate Phaseolus vulgaris involved in glycine metabolism 3-hydroxypyruvate + glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.45 Phaseolus vulgaris
-
 kidney bean, cv. Red Kidney
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.45
-
 Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.45 leaf
-
 Phaseolus vulgaris
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.45 L-serine + glyoxylate reverse reaction at 4-11% the rate of forward reaction Phaseolus vulgaris 3-hydroxypyruvate + glycine
-
 r
2.6.1.45 L-serine + glyoxylate preferred substrates Phaseolus vulgaris 3-hydroxypyruvate + glycine
-
 r
2.6.1.45 L-serine + glyoxylate involved in glycine metabolism Phaseolus vulgaris 3-hydroxypyruvate + glycine
-
 ?
2.6.1.45 L-serine + pyruvate 6% of glyoxylate transamination activity Phaseolus vulgaris 3-hydroxypyruvate + L-alanine
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.45 30
-
 assay at Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.45 8.5
-
 substrates L-alanine and 3-hydroxypyruvate Phaseolus vulgaris
2.6.1.45 9
-
 substrates glycine and 3-hydroxypyruvate Phaseolus vulgaris