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Literature summary extracted from

  • Dumora, C.; Lacoste, A.M.; Cassaigne, A.
    Purification and properties of 2-aminoethylphosphonate:pyruvate aminotransferase from Pseudomonas aeruginosa (1983), Eur. J. Biochem., 133, 119-125.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.37 4-aminobutyrate inhibitor/substrate ratio = 3, 8% inhibition Pseudomonas aeruginosa
2.6.1.37 acetate inhibitor/substrate ratio = 3, 20% inhibition Pseudomonas aeruginosa
2.6.1.37 Aminomethylphosphonate inhibitor/substrate ratio = 3, 50% inhibition Pseudomonas aeruginosa
2.6.1.37 Aminomethylsulfonate inhibitor/substrate ratio = 3, 20% inhibition Pseudomonas aeruginosa
2.6.1.37 Aminooxyacetate 1 mM, 100% inhibition Pseudomonas aeruginosa
2.6.1.37 beta-Alanine inhibitor/substrate ratio = 3, 20% inhibition Pseudomonas aeruginosa
2.6.1.37 D-cycloserine 1 mM, 40% inhibition Pseudomonas aeruginosa
2.6.1.37 DL-1-Aminobutylphosphonate inhibitor/substrate ratio = 3, 22% inhibition Pseudomonas aeruginosa
2.6.1.37 DL-1-Aminopentylphosphonate inhibitor/substrate ratio = 3, 22% inhibition Pseudomonas aeruginosa
2.6.1.37 ethylphosphonate inhibitor/substrate ratio = 3, 94% inhibition Pseudomonas aeruginosa
2.6.1.37 ethylsulfonate inhibitor/substrate ratio = 3, 12% inhibition Pseudomonas aeruginosa
2.6.1.37 HgCl2 1 mM, 100% inhibition Pseudomonas aeruginosa
2.6.1.37 hydroxylamine 1 mM, 50% inhibition Pseudomonas aeruginosa
2.6.1.37 L-cysteine 10 mM, 35% inhibition Pseudomonas aeruginosa
2.6.1.37 methylphosphonate inhibitor/substrate ratio = 3, 90% inhibition Pseudomonas aeruginosa
2.6.1.37 phenylhydrazine 1 mM, 20% inhibition Pseudomonas aeruginosa
2.6.1.37 Propylphosphonate inhibitor/substrate ratio = 3, 65% inhibition Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.37 3.5
-
pyruvate pH 8.5, 30°C Pseudomonas aeruginosa
2.6.1.37 3.85
-
2-aminoethylphosphonate pH 8.5, 30°C Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.37 16500
-
4 * 16500, SDS-PAGE Pseudomonas aeruginosa
2.6.1.37 65000
-
gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.37 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.37 poly(ethyleneimine), heat treatment, ammonium sulfate, DEAE-cellulose, hydroxyapatite, Ultrogel AcA Pseudomonas aeruginosa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.37 (2-aminoethyl)phosphonate + pyruvate = 2-phosphonoacetaldehyde + L-alanine ping-pong bi-bi mechanism Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.37 16.64
-
-
Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
2.6.1.37 -20°C, several months, no loss of activity Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.37 (2-aminoethyl)phosphonate + pyruvate highly specific for pyruvate and (2-aminoethyl)phosphonate, no activity with D-penicillamine, N-ethylmaleimide, iodoacetamide, 3-aminopropylphosphonate and taurine Pseudomonas aeruginosa 2-phosphonoacetaldehyde + L-alanine
-
?
2.6.1.37 (2-aminoethyl)phosphonate + pyruvate trivial name ciliatine Pseudomonas aeruginosa 2-phosphonoacetaldehyde + L-alanine
-
?

Subunits

EC Number Subunits Comment Organism
2.6.1.37 tetramer 4 * 16500, SDS-PAGE Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.37 50
-
-
Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.37 70
-
inactivation Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.37 8.5 9
-
Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.37 7.3 10.8 approx. 20% of maximal activity at pH 7.3, approx. 60% of maximal activity at pH 10.8, no activity above pH 11.5 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.37 pyridoxal 5'-phosphate a pyridoxal phosphate protein, 4 mol of pyridoxal phosphate per mol of enzyme Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.37 1
-
ethylphosphonate
-
Pseudomonas aeruginosa
2.6.1.37 1.55
-
methylphosphonate
-
Pseudomonas aeruginosa
2.6.1.37 2.8
-
Propylphosphonate
-
Pseudomonas aeruginosa
2.6.1.37 3.85
-
Aminomethylphosphonate
-
Pseudomonas aeruginosa