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Literature summary extracted from

  • Umemura, I.; Yanagiya, K.; Komatsubara, S.; Sato, T.; Tosa, T.
    Purification and some properties of alanine aminotransferase from Candida maltosa (1994), Biosci. Biotechnol. Biochem., 58, 283-287.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.2 Aminooxyacetate 2 mM, complete inhibition Candida maltosa
2.6.1.2 Hg2+ 0.1 mM, 81% inhibition Candida maltosa
2.6.1.2 hydroxylamine 2 mM, 73% inhibition Candida maltosa
2.6.1.2 p-hydroxymercuribenzoate 1 mM, 87% inhibition Candida maltosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.2 0.16
-
 pyruvate pH 7.5, 25°C Candida maltosa
2.6.1.2 0.37
-
 2-oxoglutarate pH 7.5, 25°C Candida maltosa
2.6.1.2 2.6
-
 L-alanine pH 7.5, 25°C Candida maltosa
2.6.1.2 6.3
-
 L-glutamate pH 7.5, 25°C Candida maltosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.2 52000
-
 2 * 52000, SDS-PAGE Candida maltosa
2.6.1.2 99000
-
 gel filtration Candida maltosa

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.2 Candida maltosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.2
-
 Candida maltosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.2 156
-
 reverse reaction Candida maltosa
2.6.1.2 594
-
 forward reaction Candida maltosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.2 L-alanine + 2-oxobutyrate 10.2% of the activity with 2-oxoglutarate Candida maltosa pyruvate + 2-aminobutanoate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Candida maltosa pyruvate + L-glutamate
-
 r
2.6.1.2 L-glutamate + 2-oxobutyrate 32.4% of the activity with pyruvate Candida maltosa 2-oxoglutarate + 2-aminobutanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.2 dimer 2 * 52000, SDS-PAGE Candida maltosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.2 55
-
-
 Candida maltosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.2 25 55 strong decrease above Candida maltosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.2 40
-
 30 min, 50% loss of activity at pH 7.5, 5% loss of activity at pH 9.0 Candida maltosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.2 6 7.5
-
 Candida maltosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.2 5.5 9.5 approx. 70% of maximal activity at pH 5.5, approx. 50% of maximal activity at pH 9.5 Candida maltosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.2 7.5
-
 40°C, 30 min, 50% loss of activity Candida maltosa
2.6.1.2 8.5 9.5 more stable at alkaline pH than at neutral pH Candida maltosa
2.6.1.2 9
-
 40°C, 30 min, 5% loss of activity Candida maltosa

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.2 pyridoxal 5'-phosphate a pyridoxal phosphate protein, 2 mol pyridoxal phosphate per mol of enzyme Candida maltosa