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Literature summary extracted from

  • Fry, S.C.; Smith, R.C.; Renwick, K.F.; Martin, D.J.; Hodge, S.K.; Matthews, K.J.
    Xyloglucan endotransglycosylase, a new wall-loosening enzyme activity from plants (1992), Biochem. J., 282, 821-828.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.207 2-mercaptoethanol slightly activates Pisum sativum
2.4.1.207 ascorbate 10 mM, slightly activates Pisum sativum
2.4.1.207 spermidine 1 mM, slighly activates Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.207 Ag+ 1 mM; partial inhibition Pisum sativum
2.4.1.207 Hg2+ 1 mM, partial inhibition Pisum sativum
2.4.1.207 La3+ 10 mM, partial inhibition Pisum sativum
2.4.1.207 additional information not inhibited by 10 mM D-gluconolactone, chelating agents, cello-oligosaccharides, cellopentaose Pisum sativum
2.4.1.207 Zn2+ 10 mM, partial inhibition Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.207 0.05
-
 Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc xyloglucan-derived nonasaccharide Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.207 additional information not ionically bound to the cell wall, little XET activity is covalently bound in the cell wall Pisum sativum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.207 Ca2+ 10 mM, slightly activates Pisum sativum
2.4.1.207 Mg2+ 10 mM, slightly activates Pisum sativum
2.4.1.207 Mn2+ 10 mM, slightly activates Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.207 donor xyloglucan + xyloglucan acceptor Zea mays
-
 ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor Solanum lycopersicum
-
 ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor Pisum sativum enzyme is responsible for cutting and rejoining intermicrofibrillar xyloglucan chains and thus causes the wall-loosening required for plant cell expansion and plant growth, in vivo the usual acceptor is polymeric wall-bound xyloglucan ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.207 Acer pseudoplatanus
-
-
-
2.4.1.207 Allium schoenoprasum
-
-
-
2.4.1.207 Anthriscus sylvestris
-
-
-
2.4.1.207 Bromus erectus
-
-
-
2.4.1.207 Holcus lanatus
-
-
-
2.4.1.207 Lupinus polyphyllus
-
-
-
2.4.1.207 Marchantia polymorpha
-
-
-
2.4.1.207 Mnium hornum
-
-
-
2.4.1.207 Pisum sativum
-
-
-
2.4.1.207 Solanum lycopersicum
-
 cv. Ailsa Craig
-
2.4.1.207 Taraxacum officinale
-
-
-
2.4.1.207 Zea mays
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.207 epicotyl
-
 Pisum sativum
-
2.4.1.207 gametophyte
-
 Mnium hornum
-
2.4.1.207 leaf
-
 Zea mays
-
2.4.1.207 leaf
-
 Solanum lycopersicum
-
2.4.1.207 leaf
-
 Lupinus polyphyllus
-
2.4.1.207 leaf
-
 Holcus lanatus
-
2.4.1.207 leaf
-
 Bromus erectus
-
2.4.1.207 leaf
-
 Anthriscus sylvestris
-
2.4.1.207 seedling
-
 Solanum lycopersicum
-
2.4.1.207 seedling 7-8-day etiolated seedlings Pisum sativum
-
2.4.1.207 stem
-
 Zea mays
-
2.4.1.207 stem
-
 Solanum lycopersicum
-
2.4.1.207 stem
-
 Holcus lanatus
-
2.4.1.207 stem
-
 Bromus erectus
-
2.4.1.207 stem etiolated stem, activity is positively correlated with growth rate in different zones of pea stem Pisum sativum
-
2.4.1.207 stem first year stem Acer pseudoplatanus
-
2.4.1.207 stem peduncle Taraxacum officinale
-
2.4.1.207 stem peduncle Allium schoenoprasum
-
2.4.1.207 thallus
-
 Marchantia polymorpha
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.207 donor xyloglucan + acceptor xyloglucan
-
 Zea mays ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan
-
 Solanum lycopersicum ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan
-
 Acer pseudoplatanus ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan
-
 Taraxacum officinale ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan
-
 Lupinus polyphyllus ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan
-
 Marchantia polymorpha ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan
-
 Mnium hornum ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan
-
 Allium schoenoprasum ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan
-
 Holcus lanatus ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan
-
 Bromus erectus ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan
-
 Anthriscus sylvestris ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan acceptor specificity Pisum sativum ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan highly specific for xyloglucan as the glycosyl donor Pisum sativum ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan minimum acceptor structure is Xyl2Glc3 Pisum sativum ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan xyloglucan from Rosa cultures Pisum sativum ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan xyloglucan from Tropaeolum majus seed Pisum sativum ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan donor specificity Pisum sativum ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan enzyme transfers a large segment of a xyloglucan molecule to another one generating chimeric polymers Pisum sativum ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan acceptors: xyloglucan-derived nonasaccharide Glc4Xyl3GalFuc and certain other xyloglucan oligosaccharides, non-reducing terminal Xyl-Glc group is essential Pisum sativum ?
-
 ?
2.4.1.207 donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc acceptor: xyloglucan-derived nonasaccharide XG9, Glc4Xyl3GalFuc, highly specific for xyloglucan as the glycosyl donor, xyloglucan from Tropaeolum seed is somewhat better than from Rosa cultures, enzyme transfers part of a large xyloglucan molecule to the nonasaccharide forming a polymer with a beta-1,4-linkage to the product and the acceptor group of the nonasaccharide is O-4 of the Glc residue furthest from the reducing terminus Pisum sativum ?
-
 ?
2.4.1.207 donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc XG9n, better substrate than Glc4Xyl3GalFuc Pisum sativum ?
-
 ?
2.4.1.207 donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc XG7, better substrate than Glc4Xyl3GalFuc Pisum sativum ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor
-
 Zea mays ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor
-
 Solanum lycopersicum ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor enzyme is responsible for cutting and rejoining intermicrofibrillar xyloglucan chains and thus causes the wall-loosening required for plant cell expansion and plant growth, in vivo the usual acceptor is polymeric wall-bound xyloglucan Pisum sativum ?
-
 ?
2.4.1.207 additional information
-
 Zea mays ?
-
 ?
2.4.1.207 additional information
-
 Solanum lycopersicum ?
-
 ?
2.4.1.207 additional information no acceptors: alpha-D-Xylp(1-6)D-Glc and isoprimeverose Pisum sativum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.207 25
-
 assay at Pisum sativum
2.4.1.207 25
-
 assay at Zea mays
2.4.1.207 25
-
 assay at Solanum lycopersicum
2.4.1.207 25
-
 assay at Acer pseudoplatanus
2.4.1.207 25
-
 assay at Taraxacum officinale
2.4.1.207 25
-
 assay at Lupinus polyphyllus
2.4.1.207 25
-
 assay at Marchantia polymorpha
2.4.1.207 25
-
 assay at Mnium hornum
2.4.1.207 25
-
 assay at Allium schoenoprasum
2.4.1.207 25
-
 assay at Holcus lanatus
2.4.1.207 25
-
 assay at Bromus erectus
2.4.1.207 25
-
 assay at Anthriscus sylvestris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.207 additional information
-
 boiling inactivates Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.207 5.5
-
-
 Pisum sativum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.207 4 7 pH 4: 25-35% of maximal activity, pH 7: 30-40% of maximal activity Pisum sativum