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Literature summary extracted from

  • Takeda, T.; Mitsuishi, Y.; Sakai, F.; Hayashi, T.
    Xyloglucan endotransglycosylation in suspension-cultured poplar cells (1996), Biosci. Biotechnol. Biochem., 60, 1950-1955.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.207 additional information
-
 additional information
-
 Populus alba
2.4.1.207 0.23
-
 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol XXXGXXXG-ol Populus alba
2.4.1.207 0.32
-
 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol XXXG-ol Populus alba

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.207 cell wall bound to Populus alba 5618
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.207 donor xyloglucan + xyloglucan acceptor Populus alba activity increases markedly at the exponential growth and decreases immediately at the stationary phase of cells in presence of 2,4-dichlorophenoxyacetic acid, the activity is developmentally regulated during growth but is not directly induced by plant hormones ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.207 Populus alba
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.207 2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc sequential mechanism Populus alba
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.207 cell suspension culture
-
 Populus alba
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.207 donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Populus alba ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan acceptors: XXXG-ol, a reduced xyloglucan heptasaccharide, its dimer XXXGXXXG-ol, and other xyloglucan oligosaccharides, reaction probably involves an enzyme-donor-acceptor complex, in which the enzyme has two binding sites separately for donor and acceptor, mechanism Populus alba ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan highly specific for xyloglucan as the glycosyl donor Populus alba ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan xyloglucan from pea Populus alba ?
-
 ?
2.4.1.207 donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol pea xyloglucan as donor, specific for xyloglucan as donor Populus alba ?
-
 ?
2.4.1.207 donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol XXXG-ol Populus alba ?
-
 ?
2.4.1.207 donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol transfers a part of xyloglucan to the reduced xyloglucan heptasaccharide XXXG-ol, the acceptor acts by combining with the enzyme independently of the donor, the velocity of the reaction decreases gradually as the heptasaccharide unit is increased from two to four, the affinity for XXXG-ol is increased at a higher concentration of donor xyloglucan Populus alba ?
-
 ?
2.4.1.207 donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol XXXGXXXG-ol, dimer of XXXG-ol, most effective acceptor, pea xyloglucan as donor Populus alba ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor activity increases markedly at the exponential growth and decreases immediately at the stationary phase of cells in presence of 2,4-dichlorophenoxyacetic acid, the activity is developmentally regulated during growth but is not directly induced by plant hormones Populus alba ?
-
 ?