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Literature summary extracted from

  • Munch-Petersen, B.; Piskur, J.; Sondergaard, L.
    Four deoxynucleoside kinase activities from Drosophila melanogaster are contained within a single monomeric enzyme, a new multifunctional deoxynucleoside kinase (1998), J. Biol. Chem., 273, 3926-3931.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.145 2'-deoxyadenosine reaction with thymidine or 2'-deoxyguanosine Drosophila melanogaster
2.7.1.145 2'-deoxycytidine competitive with thymidine Drosophila melanogaster
2.7.1.145 2'-deoxyguanosine reaction with thymidine or 2'-deoxyadenosine Drosophila melanogaster
2.7.1.145 thymidine strong inhibitor of reaction with 2'-deoxycytidine, 2'-deoxyguanosine or 2'-deoxyadenosine Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.145 0.0009
-
 thymidine pH 8.0, 22°C Drosophila melanogaster
2.7.1.145 0.001
-
 2'-deoxycytidine pH 8.0, 22°C Drosophila melanogaster
2.7.1.145 0.028
-
 1-beta-D-arabinofuranosylcytosine
-
 Drosophila melanogaster
2.7.1.145 0.109
-
 2'-deoxyadenosine pH 8.0, 22°C Drosophila melanogaster
2.7.1.145 0.654
-
 2'-deoxyguanosine pH 8.0, 22°C Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.145 30000
-
 1 * 30000, SDS-PAGE Drosophila melanogaster
2.7.1.145 33000
-
 gel filtration Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.145 Drosophila melanogaster
-
 S-2
-
2.7.1.145 Drosophila melanogaster S-2
-
 S-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.145
-
 Drosophila melanogaster

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.145 ATP + a 2'-deoxyribonucleoside = ADP + a 2'-deoxyribonucleoside 5'-phosphate compulsory ordered steady-state reaction mechanism with formation of a ternary complex with the phosphate donor and acceptor Drosophila melanogaster
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.145 embryo
-
 Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.145 29.4
-
-
 Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.145 1-beta-D-arabinofuranosylcytosine + ATP 10% of the activity with thymidine and 2'-deoxycytidine Drosophila melanogaster 1-beta-D-arabinofuranosylcytosine 5'-phosphate + ADP
-
 ?
2.7.1.145 1-beta-D-arabinofuranosylthymine + ATP 15% of the activity with thymidine and 2'-deoxycytidine Drosophila melanogaster 1-beta-D-arabinofuranosylthymine 5'-phosphate + ADP
-
 ?
2.7.1.145 2',3'-dideoxycytidine + ATP 0.02% of the activity with thymidine and 2'-deoxycytidine Drosophila melanogaster 2',3'-dideoxycytidine 5'-phosphate + ADP
-
 ?
2.7.1.145 2'-deoxyadenosine + ATP 5% of the activity with thymidine and 2'-deoxycytidine Drosophila melanogaster 2'-deoxyadenosine 5'-phosphate + ADP
-
 ?
2.7.1.145 2'-deoxycytidine + ATP
-
 Drosophila melanogaster 2'-deoxycytidine 5'-phosphate + ADP
-
 ?
2.7.1.145 2'-deoxycytidine + ATP
-
 Drosophila melanogaster S-2 2'-deoxycytidine 5'-phosphate + ADP
-
 ?
2.7.1.145 2'-deoxycytidine + CTP
-
 Drosophila melanogaster 2'-deoxycytidine 5'-phosphate + CDP
-
 ?
2.7.1.145 2'-deoxyguanosine + ATP 1% of the activity with thymidine and 2'-deoxycytidine Drosophila melanogaster 2'-deoxyguanosine 5'-phosphate + ADP
-
 ?
2.7.1.145 2'-deoxyuridine + ATP 90% of the activity with thymidine and 2'-deoxycytidine Drosophila melanogaster 2'-deoxyuridine 5'-phosphate + ADP
-
 ?
2.7.1.145 3'-azidothymidine + ATP 0.5% of the activity with thymidine and 2'-deoxycytidine Drosophila melanogaster 3'-azidothymidine 5'-phosphate + ADP
-
 ?
2.7.1.145 3'-fluoro-2',3'-dideoxythymidine + ATP 0.03% of the activity with thymidine and 2'-deoxycytidine Drosophila melanogaster 3'-fluoro-2',3'-dideoxythymidine 5'-phosphate + ADP
-
 ?
2.7.1.145 5-fluoro-2'-deoxyuridine + ATP activity is 10% higher than with thymidine and 2'-deoxycytidine Drosophila melanogaster 5-fluoro-2'-deoxyuridine 5'-phosphate + ADP
-
 ?
2.7.1.145 additional information no activity with acyclovir Drosophila melanogaster ?
-
 ?
2.7.1.145 additional information activity with CTP is 110% of the activity ATP Drosophila melanogaster ?
-
 ?
2.7.1.145 additional information no activity with dTMP Drosophila melanogaster ?
-
 ?
2.7.1.145 additional information no activity with acyclovir Drosophila melanogaster S-2 ?
-
 ?
2.7.1.145 additional information activity with CTP is 110% of the activity ATP Drosophila melanogaster S-2 ?
-
 ?
2.7.1.145 additional information no activity with dTMP Drosophila melanogaster S-2 ?
-
 ?
2.7.1.145 thymidine + ATP
-
 Drosophila melanogaster thymidine 5'-phosphate + ADP
-
 ?
2.7.1.145 thymidine + ATP
-
 Drosophila melanogaster S-2 thymidine 5'-phosphate + ADP
-
 ?
2.7.1.145 thymidine + CTP
-
 Drosophila melanogaster thymidine 5'-phosphate + CDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.145 monomer 1 * 30000, SDS-PAGE Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.145 40 60
-
 Drosophila melanogaster

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.145 15
-
 thymidine pH 8.0, 22°C Drosophila melanogaster
2.7.1.145 15
-
 2'-deoxycytidine pH 8.0, 22°C Drosophila melanogaster
2.7.1.145 18 19 2'-deoxyadenosine pH 8.0, 22°C Drosophila melanogaster
2.7.1.145 18 19 2'-deoxyguanosine pH 8.0, 22°C Drosophila melanogaster

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.145 0.001
-
 thymidine pH 8.0, 22°C, competitive with 2'-deoxycytidine Drosophila melanogaster
2.7.1.145 0.001
-
 2'-deoxycytidine pH 8.0, 22°C, competitive with thymidine Drosophila melanogaster
2.7.1.145 0.002
-
 thymidine pH 8.0, 22°C, reaction with 2'-deoxyadenosine Drosophila melanogaster
2.7.1.145 0.003
-
 thymidine pH 8.0, 22°C, reaction with 2'-deoxyguanosine Drosophila melanogaster
2.7.1.145 0.02
-
 2'-deoxyadenosine pH 8.0, 22°C, reaction with thymidine Drosophila melanogaster
2.7.1.145 0.1
-
 2'-deoxyguanosine pH 8.0, 22°C, reaction with thymidine Drosophila melanogaster
2.7.1.145 0.16
-
 2'-deoxyadenosine pH 8.0, 22°C, reaction with 2'-deoxyguanosine Drosophila melanogaster
2.7.1.145 0.4
-
 2'-deoxyguanosine pH 8.0, 22°C, reaction with 2'-deoxyadenosine Drosophila melanogaster