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Literature summary extracted from

  • Sagami, I.; Ojima, N.; Ogura, K.; Seto, S.
    Aspulvinone dimethylallyltransferase (1985), Methods Enzymol., 110, 320-326.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.35 Triton X-100 0.025%, activates 1.5times Aspergillus terreus
2.5.1.35 Tween 80 0.025%, activates 2.3times Aspergillus terreus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.35 (NH4)2SO4
-
 Aspergillus terreus
2.5.1.35 Bromophenol blue
-
 Aspergillus terreus
2.5.1.35 diphosphate
-
 Aspergillus terreus
2.5.1.35 lecithin 0.025%, 69% inhibition Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.35 0.0077
-
 aspulvinone G
-
 Aspergillus terreus
2.5.1.35 0.0137
-
 aspulvinone E
-
 Aspergillus terreus
2.5.1.35 0.04
-
 dimethylallyl diphosphate
-
 Aspergillus terreus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.35 45000
-
 6 * 45000, SDS-PAGE Aspergillus terreus
2.5.1.35 240000 270000 gel filtration Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.35 Aspergillus terreus
-
 IAM 2054
-
2.5.1.35 Aspergillus terreus IAM 2054
-
 IAM 2054
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.35
-
 Aspergillus terreus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.35 mycelium
-
 Aspergillus terreus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.35 0.0294
-
-
 Aspergillus terreus

Storage Stability

EC Number Storage Stability Organism
2.5.1.35 -20°C, stable for 1 week Aspergillus terreus
2.5.1.35 4°C, 50% loss of activity after 3 weeks Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.35 (E)-3-methylpent-2-enyl diphosphate + aspulvinone E
-
 Aspergillus terreus ?
-
 ?
2.5.1.35 (E)-3-methylpent-2-enyl diphosphate + aspulvinone E
-
 Aspergillus terreus IAM 2054 ?
-
 ?
2.5.1.35 (E)-butenyl diphosphate + aspulvinone E
-
 Aspergillus terreus ?
-
 ?
2.5.1.35 (E)-butenyl diphosphate + aspulvinone E
-
 Aspergillus terreus IAM 2054 ?
-
 ?
2.5.1.35 cycloheptylideneethyl diphosphate + aspulvinone E
-
 Aspergillus terreus ?
-
 ?
2.5.1.35 cyclohexylideneethyl diphosphate + aspulvinone E
-
 Aspergillus terreus ?
-
 ?
2.5.1.35 cyclohexylideneethyl diphosphate + aspulvinone E
-
 Aspergillus terreus IAM 2054 ?
-
 ?
2.5.1.35 cyclopentylideneethyl diphosphate + aspulvinone E
-
 Aspergillus terreus ?
-
 ?
2.5.1.35 cyclopentylideneethyl diphosphate + aspulvinone E
-
 Aspergillus terreus IAM 2054 ?
-
 ?
2.5.1.35 dimethylallyl diphosphate + aspulvinone E i.e. dihydroxypulvinone Aspergillus terreus diphosphate + aspulvinone H
-
 ?
2.5.1.35 dimethylallyl diphosphate + aspulvinone E i.e. dihydroxypulvinone Aspergillus terreus IAM 2054 diphosphate + aspulvinone H
-
 ?
2.5.1.35 dimethylallyl diphosphate + aspulvinone G i.e. trihydroxypulvinone, 71% of the activity with aspulvinone E Aspergillus terreus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.35 hexamer 6 * 45000, SDS-PAGE Aspergillus terreus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.35 0.0178
-
 diphosphate
-
 Aspergillus terreus
2.5.1.35 0.05
-
 diphosphate
-
 Aspergillus terreus