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Literature summary extracted from

  • Takada, Y.; Noguchi, T.
    Aromatic-amino acid-glyoxylate aminotransferase from rat liver (1987), Methods Enzymol., 142, 273-279.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.60 hydroxylamine
-
 Rattus norvegicus
2.6.1.60 Isonicotinic hydrazide
-
 Rattus norvegicus
2.6.1.60 KCN
-
 Rattus norvegicus
2.6.1.60 Semicarbazide strong Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.60 0.07
-
 glyoxylate cosubstrate L-alanine, 37°C Rattus norvegicus
2.6.1.60 0.07
-
 pyruvate cosubstrate L-serine, 37°C Rattus norvegicus
2.6.1.60 1.5
-
 pyruvate cosubstrate L-histidine, 37°C Rattus norvegicus
2.6.1.60 2.1
-
 alanine cosubstrate L-glyoxylate, 37°C Rattus norvegicus
2.6.1.60 2.6
-
 phenylalanine cosubstrate pyruvate, 37°C Rattus norvegicus
2.6.1.60 2.7
-
 histidine cosubstrate pyruvate, 37°C Rattus norvegicus
2.6.1.60 5.1
-
 pyruvate cosubstrate L-phenylalanine, 37°C Rattus norvegicus
2.6.1.60 16
-
 serine cosubstrate pyruvate, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.60 mitochondrion
-
 Mammalia 5739
-
2.6.1.60 mitochondrion matrix Rattus norvegicus 5739
-
2.6.1.60 additional information subcellular distribution distribution in different mammalia Mammalia
-
-
2.6.1.60 peroxisome
-
 Mammalia 5777
-
2.6.1.60 peroxisome matrix and soluble Rattus norvegicus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.60 40000
-
 2 * 40000, SDS-PAGE Rattus norvegicus
2.6.1.60 80000
-
 gel filtration Rattus norvegicus
2.6.1.60 80000
-
 sucrose density gradient centrifugation Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.60 Mammalia
-
 distribution among mammals
-
2.6.1.60 Rattus norvegicus
-
 mitochondrial enzyme inducible by glucagon or cAMP, peroxisomal enzyme inducible by clofibrate
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.60
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.60 liver
-
 Mammalia
-
2.6.1.60 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.60 12.9
-
 substrates histidine + pyruvate, 37°C Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.6.1.60 -20°C, at least 6 weeks Rattus norvegicus
2.6.1.60 0-5°C, 2 weeks with little or no loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.60 asparagine + glyoxylate less efficient amino group donor than L-phenylalanine Rattus norvegicus 2-oxosuccinamate + glycine
-
 ?
2.6.1.60 glutamine + glyoxylate less efficient amino group donor than L-phenylalanine Rattus norvegicus 4-carbamoyl-2-oxobutanoate + glycine
-
 ?
2.6.1.60 isoleucine + glyoxylate poor substrate Rattus norvegicus 3-methyl-2-oxopentanoate + glycine
-
 ?
2.6.1.60 L-glutamate + glyoxylate poor substrate Rattus norvegicus 2-oxoglutarate + glycine
-
 ?
2.6.1.60 L-histidine + glyoxylate less efficient amino group donor than L-phenylalanine Rattus norvegicus 3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
 ?
2.6.1.60 L-kynurenine + glyoxylate
-
 Rattus norvegicus 3-(2-aminophenyl-)2,4-dioxobutanoate + glycine
-
 ?
2.6.1.60 L-leucine + glyoxylate less efficient amino group donor than L-phenylalanine Rattus norvegicus 4-methyl-2-oxopentanoate + glycine
-
 ?
2.6.1.60 L-methionine + glyoxylate less efficient amino group donor than L-phenylalanine Rattus norvegicus 4-methylsulfanyl-2-oxobutanoate + glycine
-
 ?
2.6.1.60 L-phenylalanine + 2-oxo-4-methylthiobutyrate
-
 Rattus norvegicus phenylpyruvate + L-methionine
-
 ?
2.6.1.60 L-phenylalanine + glyoxylate
-
 Rattus norvegicus phenylpyruvate + glycine
-
 ?
2.6.1.60 L-phenylalanine + hydroxypyruvate
-
 Rattus norvegicus phenylpyruvate + L-serine
-
 ?
2.6.1.60 L-phenylalanine + pyruvate
-
 Rattus norvegicus phenylpyruvate + L-alanine
-
 ?
2.6.1.60 L-serine + glyoxylate less efficient amino group donor than L-phenylalanine Rattus norvegicus 3-hydroxy-2-oxopropanoate + glycine
-
 ?
2.6.1.60 tyrosine + glyoxylate less efficient amino group donor than L-phenylalanine Rattus norvegicus 3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
-
 ?
2.6.1.60 valine + glyoxylate poor substrate Rattus norvegicus 3-methyl-2-oxobutanoate + glycine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.60 dimer 2 * 40000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.60 37
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.60 pyridoxal 5'-phosphate requirement Rattus norvegicus