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Literature summary extracted from

  • Van Cauwenberghe, O.R.; Shelp, B.J.
    Biochemical characterization of partially purified gaba:pyruvate transaminase from Nicotiana tabacum (1999), Phytochemistry, 52, 575-581.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.19 Aminooxyacetate 80% inhibition at 2 mM Nicotiana tabacum
2.6.1.19 gabaculine 80% inhibition at 2 mM Nicotiana tabacum
2.6.1.19 Succinic semialdehyde
-
 Nicotiana tabacum
2.6.1.96 aminooxyacetic acid 80% inhibition at 2 mM Nicotiana tabacum
2.6.1.96 beta-Alanine 30% inhibition at 2 mM Nicotiana tabacum
2.6.1.96 gabaculine 80% inhibition at 2 mM Nicotiana tabacum
2.6.1.96 L-glutamine 11% inhibition at 2 mM Nicotiana tabacum
2.6.1.96 L-ornithine 40% inhibition at 2 mM Nicotiana tabacum
2.6.1.96 additional information no or poor inhibition by proline and succinate Nicotiana tabacum
2.6.1.96 Succinic semialdehyde 67% inhibition at 2 mM Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.19 0.24
-
 pyruvate pH 8.2 Nicotiana tabacum
2.6.1.19 1.2
-
 4-aminobutanoate pH 8.2 Nicotiana tabacum
2.6.1.96 0.24
-
 pyruvate pH 8.2, temperature not specified in the publication Nicotiana tabacum
2.6.1.96 1.2
-
 4-aminobutanoate pH 8.2, temperature not specified in the publication Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.19 mitochondrion
-
 Nicotiana tabacum 5739
-
2.6.1.96 mitochondrion
-
 Nicotiana tabacum 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.96 4-aminobutanoate + pyruvate Nicotiana tabacum
-
 succinate semialdehyde + L-alanine
-
 r
2.6.1.96 4-aminobutanoate + pyruvate Nicotiana tabacum Samsun N.N.
-
 succinate semialdehyde + L-alanine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.19 Nicotiana tabacum
-
-
-
2.6.1.96 Nicotiana tabacum
-
-
-
2.6.1.96 Nicotiana tabacum Samsun N.N.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.19 partial Nicotiana tabacum
2.6.1.96 the pyruvate-dependent GABA-T activity is separated from 2-oxoglutarate-dependent GABA-T activity, EC 2.6.1.19, by two steps of anion exchange chromatography, purification 1530fold Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.96 leaf
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.96 0.17
-
 purified enzyme, pH 8.2, temperature not specified in the publication Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.19 4-aminobutanoate + 2-oxoglutarate no substrate: 2-oxoglutarate Nicotiana tabacum 4-oxobutanoate + L-glutamate
-
 ?
2.6.1.19 4-aminobutanoate + 2-oxoglutarate no substrate: 2-oxoglutarate Nicotiana tabacum Samsun N.N. 4-oxobutanoate + L-glutamate
-
 ?
2.6.1.19 4-aminobutanoate + pyruvate not using 2-oxoglutarate Nicotiana tabacum 4-oxobutanoate + alanine
-
 ?
2.6.1.19 4-aminobutanoate + pyruvate not using 2-oxoglutarate Nicotiana tabacum Samsun N.N. 4-oxobutanoate + alanine
-
 ?
2.6.1.96 4-aminobutanoate + pyruvate
-
 Nicotiana tabacum succinate semialdehyde + L-alanine
-
 r
2.6.1.96 4-aminobutanoate + pyruvate pyruvate-specific mitochondrial GABA-T Nicotiana tabacum succinate semialdehyde + L-alanine
-
 r
2.6.1.96 4-aminobutanoate + pyruvate
-
 Nicotiana tabacum Samsun N.N. succinate semialdehyde + L-alanine
-
 r
2.6.1.96 4-aminobutanoate + pyruvate pyruvate-specific mitochondrial GABA-T Nicotiana tabacum Samsun N.N. succinate semialdehyde + L-alanine
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.6.1.96 4-aminobutyrate transaminase
-
 Nicotiana tabacum
2.6.1.96 gaba:pyruvate transaminase
-
 Nicotiana tabacum
2.6.1.96 pyruvate-specific mitochondrial GABA-T
-
 Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.96 8.2
-
 assay at Nicotiana tabacum

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.96 pyridoxal 5'-phosphate
-
 Nicotiana tabacum