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Literature summary extracted from

  • Maul, D.M.; Schuster, S.M.
    Kinetic properties and characteristics of mouse liver mitochondrial asparagine aminotransferase (1986), Arch. Biochem. Biophys., 251, 585-593.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.14 (NH4)2SO4 at high concentration Mus musculus
2.6.1.14 AgNO3
-
 Mus musculus
2.6.1.14 Aminooxyacetate
-
 Mus musculus
2.6.1.14 D-cycloserine weak Mus musculus
2.6.1.14 glycine asparagine/pyruvate reaction Mus musculus
2.6.1.14 HgCl2
-
 Mus musculus
2.6.1.14 homoserine asparagine/pyruvate reaction Mus musculus
2.6.1.14 hydroxylamine
-
 Mus musculus
2.6.1.14 KCN
-
 Mus musculus
2.6.1.14 L-aspartate weak, asparagine/pyruvate reaction Mus musculus
2.6.1.14 L-cysteine
-
 Mus musculus
2.6.1.14 L-glutamate weak, asparagine/pyruvate reaction Mus musculus
2.6.1.14 L-glutamine asparagine/pyruvate reaction Mus musculus
2.6.1.14 leucine asparagine/pyruvate reaction Mus musculus
2.6.1.14 methionine asparagine/pyruvate reaction Mus musculus
2.6.1.14 additional information strong substrate and product inhibition Mus musculus
2.6.1.14 N-ethylmaleimide
-
 Mus musculus
2.6.1.14 norvaline asparagine/pyruvate reaction Mus musculus
2.6.1.14 p-chloromercuribenzoate
-
 Mus musculus
2.6.1.14 phenylalanine
-
 Mus musculus
2.6.1.14 serine asparagine/pyruvate reaction Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.14 0.03
-
 L-methionine cosubstrate phenylpyruvate Mus musculus
2.6.1.14 0.43
-
 L-glutamine cosubstrate phenylpyruvate Mus musculus
2.6.1.14 1
-
 L-asparagine cosubstrate phenylpyruvate Mus musculus
2.6.1.14 1.4
-
 L-homoserine cosubstrate phenylpyruvate Mus musculus
2.6.1.14 4.8
-
 L-alanine cosubstrate 2-oxosuccinamate Mus musculus
2.6.1.14 6.5
-
 L-norvaline cosubstrate 2-oxosuccinamate Mus musculus
2.6.1.14 6.8
-
 L-methionine cosubstrate 2-oxosuccinamate Mus musculus
2.6.1.14 21.4
-
 L-glutamine cosubstrate 2-oxosuccinamate Mus musculus
2.6.1.14 24.7
-
 L-homocysteine cosubstrate 2-oxosuccinamate Mus musculus
2.6.1.14 28.7
-
 L-leucine cosubstrate 2-oxosuccinamate Mus musculus
2.6.1.14 28.8
-
 L-2-aminobutanoic acid cosubstrate 2-oxosuccinamate Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.14 cytosol
-
 Mus musculus 5829
-
2.6.1.14 mitochondrion
-
 Mus musculus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.14 70800
-
 mouse mitochondrial enzyme Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.14 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.14
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.14 liver
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.14 additional information
-
 highest activity at pH 8.5 in N,N-bis(2-hydroxyethyl)glycine buffer and 4-(2-hydroxyethyl)-1-piperazinepropane sulfonic acid buffer, low activity in borate buffer and glycylglycine buffer Mus musculus
2.6.1.14 5.08
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.14 2-oxosuccinamic acid + L-2-aminobutanoic acid
-
 Mus musculus L-asparagine + 2-oxobutanoate
-
 ?
2.6.1.14 2-oxosuccinamic acid + L-alanine
-
 Mus musculus L-asparagine + pyruvate
-
 ?
2.6.1.14 2-oxosuccinamic acid + L-glutamine
-
 Mus musculus L-asparagine + L-glutamate
-
 ?
2.6.1.14 2-oxosuccinamic acid + L-homocysteine
-
 Mus musculus L-asparagine + 4-mercapto-2-oxobutanoate
-
 ?
2.6.1.14 2-oxosuccinamic acid + L-leucine
-
 Mus musculus L-asparagine + 4-methyl-2-oxopentanoate
-
 ?
2.6.1.14 2-oxosuccinamic acid + L-methionine
-
 Mus musculus L-asparagine + 4-methylsulfanyl-2-oxobutanoate
-
 r
2.6.1.14 2-oxosuccinamic acid + L-norvaline
-
 Mus musculus L-asparagine + 2-oxopentanoate
-
 ?
2.6.1.14 L-asparagine + 2-oxoisohexanoate
-
 Mus musculus 2-oxosuccinamate + 2-aminoisohexanoate
-
 ?
2.6.1.14 L-asparagine + 4-hydroxy-2-oxoglutarate
-
 Mus musculus 2-oxosuccinamate + 2-amino-4-hydroxyglutarate
-
 ?
2.6.1.14 L-asparagine + 4-methylsulfanyl-2-oxobutanoate weak Mus musculus 2-oxosuccinamate + L-methionine
-
 r
2.6.1.14 L-asparagine + glyoxylate
-
 Mus musculus 2-oxosuccinamate + glycine
-
 ?
2.6.1.14 L-asparagine + pyruvate
-
 Mus musculus 2-oxosuccinamate + L-alanine
-
 ?
2.6.1.14 phenylpyruvate + L-asparagine
-
 Mus musculus L-phenylalanine + 2-oxosuccinamate
-
 ?
2.6.1.14 phenylpyruvate + L-glutamine
-
 Mus musculus L-phenylalanine + 2-oxoglutaramate
-
 ?
2.6.1.14 phenylpyruvate + L-homoserine
-
 Mus musculus phenylalanine + 4-hydroxy-2-oxobutanoate
-
 ?
2.6.1.14 phenylpyruvate + methionine
-
 Mus musculus L-phenylalanine + 4-methylsulfanyl-2-oxobutanoate
-
 ?