Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Becker, M.A.; Kredich, N.M.; Tomkins, G.M.
    The purification and characterization of O-acetylserine sulfhydrylase-A from Salmonella typhimurium (1969), J. Biol. Chem., 244, 2418-2427.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.47 L-cysteine not inhibitory up to 3.7 mM Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 5
-
 O-acetyl-L-Ser free enzyme Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 5
-
 O-acetyl-L-Ser pH 7.2-7.4, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 5
-
 O-acetyl-L-Ser independent of sulfide concentration Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 34000
-
 2 * 34000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 68000
-
 free enzyme, sedimentation equilibrium Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.47 O-acetyl-L-Ser + sulfide Salmonella enterica subsp. enterica serovar Typhimurium final step in Cys synthesis L-Cys + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 1100
-
 free enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 L-Cys + acetate equilibrium constant Salmonella enterica subsp. enterica serovar Typhimurium O-acetyl-L-Ser + H2S
-
 r
2.5.1.47 O-acetyl-L-Ser + H2S equilibrium constant Salmonella enterica subsp. enterica serovar Typhimurium L-Cys + acetate
-
 r
2.5.1.47 O-acetyl-L-Ser + sulfide final step in Cys synthesis Salmonella enterica subsp. enterica serovar Typhimurium L-Cys + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 dimer 2 * 34000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 More amino acid analysis, N-terminal amino acid is serine Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.47 5.8 9.4
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.47 pyridoxal 5'-phosphate 2.1 mol per mol of dimeric enzyme Salmonella enterica subsp. enterica serovar Typhimurium